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Lymphocyte subsets in human immunodeficiency virus-unexposed Brazilian individuals from birth to adulthood.

Moraes-Pinto MI, Ono E, Santos-Valente EC, Almeida LC, Andrade PR, Dinelli MI, Santos AM, Salomão R - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: Ethnic origin, genetics, gender and environmental factors have been shown to influence some immunologic indices, so that development of reference values for populations of different backgrounds may be necessary.CD38 expression on both CD4+ and CD8+ T cells was lower than the PACTG P1009 values, with a widening gap between the two studies at older age ranges.Different patterns of cell differentiation seem to occur in different settings and may have characteristic expression within each population.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
Ethnic origin, genetics, gender and environmental factors have been shown to influence some immunologic indices, so that development of reference values for populations of different backgrounds may be necessary. We have determined the distribution of lymphocyte subsets in healthy Brazilian individuals from birth to adulthood. Lymphocyte subsets were determined using four-colour cytometry in a cross-sectional study of 463 human immunodeficiency virus-unexposed children and adults from birth through 49 years of age. Lymphocyte subsets varied according to age, as previously observed in other studies. However, total CD4+ T cell numbers were lower than what was described in the Pediatric AIDS Clinical Trials Group P1009 (PACTG P1009), which assessed an American population of predominantly African and Hispanic backgrounds until the 12-18 year age range, when values were comparable. Naïve percentages and absolute values of CD8+ T cells, as assessed by CD45RA expression, were also lower than the PACTG P1009 data for all analysed age ranges. CD38 expression on both CD4+ and CD8+ T cells was lower than the PACTG P1009 values, with a widening gap between the two studies at older age ranges. Different patterns of cell differentiation seem to occur in different settings and may have characteristic expression within each population.

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: total lymphocyte, CD3+ T cell, CD4+ T cell, CD8+ T cell, B cell andnatural killer (NK) cell counts in American children and adolescents fromPediatric AIDS Clinical Trials Group P1009 (PACTG P1009) study and fromBrazilian children and adolescents distributed in similar age strata. Data arepresented in median values for each age stratum, with 95% confidence interval(CI) shown for the Brazilian cohort. Study data are considered statisticallydifferent if median values from PACTG P1009 study are not included in the 95%CI of the Brazilian cohort. m: months; y: years.
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f02: : total lymphocyte, CD3+ T cell, CD4+ T cell, CD8+ T cell, B cell andnatural killer (NK) cell counts in American children and adolescents fromPediatric AIDS Clinical Trials Group P1009 (PACTG P1009) study and fromBrazilian children and adolescents distributed in similar age strata. Data arepresented in median values for each age stratum, with 95% confidence interval(CI) shown for the Brazilian cohort. Study data are considered statisticallydifferent if median values from PACTG P1009 study are not included in the 95%CI of the Brazilian cohort. m: months; y: years.

Mentions: Results from the present study were compared with data from PACTG P1009 (Shearer et al. 2003). Parameters compared were thosethat were tested by both studies within age strata from zero-three months to 12-18years. They were: total lymphocyte, CD3+ T cell, CD4+ T cell,CD8+ T cell, B cell and NK cell counts (Fig. 2), CD4+ T cells and CD8+ T cells were alsocompared by percentage and absolute values of naïve subsets (CD45RA+) andpercentage of CD38+ cells (Fig. 3).


Lymphocyte subsets in human immunodeficiency virus-unexposed Brazilian individuals from birth to adulthood.

Moraes-Pinto MI, Ono E, Santos-Valente EC, Almeida LC, Andrade PR, Dinelli MI, Santos AM, Salomão R - Mem. Inst. Oswaldo Cruz (2014)

: total lymphocyte, CD3+ T cell, CD4+ T cell, CD8+ T cell, B cell andnatural killer (NK) cell counts in American children and adolescents fromPediatric AIDS Clinical Trials Group P1009 (PACTG P1009) study and fromBrazilian children and adolescents distributed in similar age strata. Data arepresented in median values for each age stratum, with 95% confidence interval(CI) shown for the Brazilian cohort. Study data are considered statisticallydifferent if median values from PACTG P1009 study are not included in the 95%CI of the Brazilian cohort. m: months; y: years.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325616&req=5

f02: : total lymphocyte, CD3+ T cell, CD4+ T cell, CD8+ T cell, B cell andnatural killer (NK) cell counts in American children and adolescents fromPediatric AIDS Clinical Trials Group P1009 (PACTG P1009) study and fromBrazilian children and adolescents distributed in similar age strata. Data arepresented in median values for each age stratum, with 95% confidence interval(CI) shown for the Brazilian cohort. Study data are considered statisticallydifferent if median values from PACTG P1009 study are not included in the 95%CI of the Brazilian cohort. m: months; y: years.
Mentions: Results from the present study were compared with data from PACTG P1009 (Shearer et al. 2003). Parameters compared were thosethat were tested by both studies within age strata from zero-three months to 12-18years. They were: total lymphocyte, CD3+ T cell, CD4+ T cell,CD8+ T cell, B cell and NK cell counts (Fig. 2), CD4+ T cells and CD8+ T cells were alsocompared by percentage and absolute values of naïve subsets (CD45RA+) andpercentage of CD38+ cells (Fig. 3).

Bottom Line: Ethnic origin, genetics, gender and environmental factors have been shown to influence some immunologic indices, so that development of reference values for populations of different backgrounds may be necessary.CD38 expression on both CD4+ and CD8+ T cells was lower than the PACTG P1009 values, with a widening gap between the two studies at older age ranges.Different patterns of cell differentiation seem to occur in different settings and may have characteristic expression within each population.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
Ethnic origin, genetics, gender and environmental factors have been shown to influence some immunologic indices, so that development of reference values for populations of different backgrounds may be necessary. We have determined the distribution of lymphocyte subsets in healthy Brazilian individuals from birth to adulthood. Lymphocyte subsets were determined using four-colour cytometry in a cross-sectional study of 463 human immunodeficiency virus-unexposed children and adults from birth through 49 years of age. Lymphocyte subsets varied according to age, as previously observed in other studies. However, total CD4+ T cell numbers were lower than what was described in the Pediatric AIDS Clinical Trials Group P1009 (PACTG P1009), which assessed an American population of predominantly African and Hispanic backgrounds until the 12-18 year age range, when values were comparable. Naïve percentages and absolute values of CD8+ T cells, as assessed by CD45RA expression, were also lower than the PACTG P1009 data for all analysed age ranges. CD38 expression on both CD4+ and CD8+ T cells was lower than the PACTG P1009 values, with a widening gap between the two studies at older age ranges. Different patterns of cell differentiation seem to occur in different settings and may have characteristic expression within each population.

Show MeSH
Related in: MedlinePlus