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Lymphocyte subsets in human immunodeficiency virus-unexposed Brazilian individuals from birth to adulthood.

Moraes-Pinto MI, Ono E, Santos-Valente EC, Almeida LC, Andrade PR, Dinelli MI, Santos AM, Salomão R - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: Ethnic origin, genetics, gender and environmental factors have been shown to influence some immunologic indices, so that development of reference values for populations of different backgrounds may be necessary.CD38 expression on both CD4+ and CD8+ T cells was lower than the PACTG P1009 values, with a widening gap between the two studies at older age ranges.Different patterns of cell differentiation seem to occur in different settings and may have characteristic expression within each population.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
Ethnic origin, genetics, gender and environmental factors have been shown to influence some immunologic indices, so that development of reference values for populations of different backgrounds may be necessary. We have determined the distribution of lymphocyte subsets in healthy Brazilian individuals from birth to adulthood. Lymphocyte subsets were determined using four-colour cytometry in a cross-sectional study of 463 human immunodeficiency virus-unexposed children and adults from birth through 49 years of age. Lymphocyte subsets varied according to age, as previously observed in other studies. However, total CD4+ T cell numbers were lower than what was described in the Pediatric AIDS Clinical Trials Group P1009 (PACTG P1009), which assessed an American population of predominantly African and Hispanic backgrounds until the 12-18 year age range, when values were comparable. Naïve percentages and absolute values of CD8+ T cells, as assessed by CD45RA expression, were also lower than the PACTG P1009 data for all analysed age ranges. CD38 expression on both CD4+ and CD8+ T cells was lower than the PACTG P1009 values, with a widening gap between the two studies at older age ranges. Different patterns of cell differentiation seem to occur in different settings and may have characteristic expression within each population.

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Related in: MedlinePlus

: illustrative example of flow cytometry plots for data analysis.Lymphocytes were selected on the side scatter/forward scatter plot with a gateas shown in A. T cells were identified from the gate of lymphocytes as CD3+(B), B cells, as CD3-CD19+ (C) and natural killer (NK) cells, asCD3-CD16+/CD56+ (D). CD4+ T cells were identified from the gate of lymphocytesas CD3+CD4+ (E) and then separated in differentiation subsets using CD45RA/CCR7(F) or CD45RA/CD27 markers (G); CD38 and CD25 expression were evaluated on CD4+T cells in H and I, respectively. CD8+ T cells were identified from the gate oflymphocytes as CD3+CD8+ (J) and then separated in differentiation subsets usingCD45RA/CCR7 (K) or CD45RA/CD27 markers (L); CD38 and CD56 expression wereevaluated on CD8+ T cells in M and N, respectively. Isotypic controls wereemployed to set the gates in F-I, K-N plots.
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f01: : illustrative example of flow cytometry plots for data analysis.Lymphocytes were selected on the side scatter/forward scatter plot with a gateas shown in A. T cells were identified from the gate of lymphocytes as CD3+(B), B cells, as CD3-CD19+ (C) and natural killer (NK) cells, asCD3-CD16+/CD56+ (D). CD4+ T cells were identified from the gate of lymphocytesas CD3+CD4+ (E) and then separated in differentiation subsets using CD45RA/CCR7(F) or CD45RA/CD27 markers (G); CD38 and CD25 expression were evaluated on CD4+T cells in H and I, respectively. CD8+ T cells were identified from the gate oflymphocytes as CD3+CD8+ (J) and then separated in differentiation subsets usingCD45RA/CCR7 (K) or CD45RA/CD27 markers (L); CD38 and CD56 expression wereevaluated on CD8+ T cells in M and N, respectively. Isotypic controls wereemployed to set the gates in F-I, K-N plots.

Mentions: Fig. 1 shows an illustrative example of flowcytometry plots used for data analysis.


Lymphocyte subsets in human immunodeficiency virus-unexposed Brazilian individuals from birth to adulthood.

Moraes-Pinto MI, Ono E, Santos-Valente EC, Almeida LC, Andrade PR, Dinelli MI, Santos AM, Salomão R - Mem. Inst. Oswaldo Cruz (2014)

: illustrative example of flow cytometry plots for data analysis.Lymphocytes were selected on the side scatter/forward scatter plot with a gateas shown in A. T cells were identified from the gate of lymphocytes as CD3+(B), B cells, as CD3-CD19+ (C) and natural killer (NK) cells, asCD3-CD16+/CD56+ (D). CD4+ T cells were identified from the gate of lymphocytesas CD3+CD4+ (E) and then separated in differentiation subsets using CD45RA/CCR7(F) or CD45RA/CD27 markers (G); CD38 and CD25 expression were evaluated on CD4+T cells in H and I, respectively. CD8+ T cells were identified from the gate oflymphocytes as CD3+CD8+ (J) and then separated in differentiation subsets usingCD45RA/CCR7 (K) or CD45RA/CD27 markers (L); CD38 and CD56 expression wereevaluated on CD8+ T cells in M and N, respectively. Isotypic controls wereemployed to set the gates in F-I, K-N plots.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325616&req=5

f01: : illustrative example of flow cytometry plots for data analysis.Lymphocytes were selected on the side scatter/forward scatter plot with a gateas shown in A. T cells were identified from the gate of lymphocytes as CD3+(B), B cells, as CD3-CD19+ (C) and natural killer (NK) cells, asCD3-CD16+/CD56+ (D). CD4+ T cells were identified from the gate of lymphocytesas CD3+CD4+ (E) and then separated in differentiation subsets using CD45RA/CCR7(F) or CD45RA/CD27 markers (G); CD38 and CD25 expression were evaluated on CD4+T cells in H and I, respectively. CD8+ T cells were identified from the gate oflymphocytes as CD3+CD8+ (J) and then separated in differentiation subsets usingCD45RA/CCR7 (K) or CD45RA/CD27 markers (L); CD38 and CD56 expression wereevaluated on CD8+ T cells in M and N, respectively. Isotypic controls wereemployed to set the gates in F-I, K-N plots.
Mentions: Fig. 1 shows an illustrative example of flowcytometry plots used for data analysis.

Bottom Line: Ethnic origin, genetics, gender and environmental factors have been shown to influence some immunologic indices, so that development of reference values for populations of different backgrounds may be necessary.CD38 expression on both CD4+ and CD8+ T cells was lower than the PACTG P1009 values, with a widening gap between the two studies at older age ranges.Different patterns of cell differentiation seem to occur in different settings and may have characteristic expression within each population.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
Ethnic origin, genetics, gender and environmental factors have been shown to influence some immunologic indices, so that development of reference values for populations of different backgrounds may be necessary. We have determined the distribution of lymphocyte subsets in healthy Brazilian individuals from birth to adulthood. Lymphocyte subsets were determined using four-colour cytometry in a cross-sectional study of 463 human immunodeficiency virus-unexposed children and adults from birth through 49 years of age. Lymphocyte subsets varied according to age, as previously observed in other studies. However, total CD4+ T cell numbers were lower than what was described in the Pediatric AIDS Clinical Trials Group P1009 (PACTG P1009), which assessed an American population of predominantly African and Hispanic backgrounds until the 12-18 year age range, when values were comparable. Naïve percentages and absolute values of CD8+ T cells, as assessed by CD45RA expression, were also lower than the PACTG P1009 data for all analysed age ranges. CD38 expression on both CD4+ and CD8+ T cells was lower than the PACTG P1009 values, with a widening gap between the two studies at older age ranges. Different patterns of cell differentiation seem to occur in different settings and may have characteristic expression within each population.

Show MeSH
Related in: MedlinePlus