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identification of Pseudomonas spp. as amoeba-resistant microorganisms in isolates of Acanthamoeba.

José Maschio V, Corção G, Rott MB - Rev. Inst. Med. Trop. Sao Paulo (2015 Jan-Feb)

Bottom Line: The aim of this study was to identify the presence of Pseudomonas as an amoeba-resistant microorganism in 12 isolates of Acanthamoeba.All isolates showed the genus Pseudomonas spp. as amoeba-resistant microorganisms.Thus, one can see that the Acanthamoeba isolates studied are hosts of Pseudomonas.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiologia, Imunologia e Parasitologia. Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

ABSTRACT
Acanthamoeba is a "Trojan horse" of the microbial world. The aim of this study was to identify the presence of Pseudomonas as an amoeba-resistant microorganism in 12 isolates of Acanthamoeba. All isolates showed the genus Pseudomonas spp. as amoeba-resistant microorganisms. Thus, one can see that the Acanthamoeba isolates studied are hosts of Pseudomonas.

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Samples of Acanthamoeba A1, A6, A9, A11 and A12 isolated fromcontact lens cases, and A2, A3, A4, A5, A7, A8, and A10 isolated from airconditioning units. Positive control (PC) strain of Pseudomonasaeruginosa ATCC 278532.
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f01: Samples of Acanthamoeba A1, A6, A9, A11 and A12 isolated fromcontact lens cases, and A2, A3, A4, A5, A7, A8, and A10 isolated from airconditioning units. Positive control (PC) strain of Pseudomonasaeruginosa ATCC 278532.

Mentions: In the present study, all isolates of Acanthamoeba showedinternalized bacteria when primers are used to amplify the Bacteria domain and all isolatesshowed the genus Pseudomonas spp. as amoeba-resistant microorganisms(Fig. 1). A total of six PCR products (Ap1 to Ap6)were sent for sequencing (Table 1) and all wereconfirmed as Pseudomonas spp.


identification of Pseudomonas spp. as amoeba-resistant microorganisms in isolates of Acanthamoeba.

José Maschio V, Corção G, Rott MB - Rev. Inst. Med. Trop. Sao Paulo (2015 Jan-Feb)

Samples of Acanthamoeba A1, A6, A9, A11 and A12 isolated fromcontact lens cases, and A2, A3, A4, A5, A7, A8, and A10 isolated from airconditioning units. Positive control (PC) strain of Pseudomonasaeruginosa ATCC 278532.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325528&req=5

f01: Samples of Acanthamoeba A1, A6, A9, A11 and A12 isolated fromcontact lens cases, and A2, A3, A4, A5, A7, A8, and A10 isolated from airconditioning units. Positive control (PC) strain of Pseudomonasaeruginosa ATCC 278532.
Mentions: In the present study, all isolates of Acanthamoeba showedinternalized bacteria when primers are used to amplify the Bacteria domain and all isolatesshowed the genus Pseudomonas spp. as amoeba-resistant microorganisms(Fig. 1). A total of six PCR products (Ap1 to Ap6)were sent for sequencing (Table 1) and all wereconfirmed as Pseudomonas spp.

Bottom Line: The aim of this study was to identify the presence of Pseudomonas as an amoeba-resistant microorganism in 12 isolates of Acanthamoeba.All isolates showed the genus Pseudomonas spp. as amoeba-resistant microorganisms.Thus, one can see that the Acanthamoeba isolates studied are hosts of Pseudomonas.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiologia, Imunologia e Parasitologia. Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

ABSTRACT
Acanthamoeba is a "Trojan horse" of the microbial world. The aim of this study was to identify the presence of Pseudomonas as an amoeba-resistant microorganism in 12 isolates of Acanthamoeba. All isolates showed the genus Pseudomonas spp. as amoeba-resistant microorganisms. Thus, one can see that the Acanthamoeba isolates studied are hosts of Pseudomonas.

Show MeSH
Related in: MedlinePlus