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Membrane fractions from Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis.

Corral MA, Paula FM, Gottardi M, Meisel DM, Chieffi PP, Gryschek RC - Rev. Inst. Med. Trop. Sao Paulo (2015 Jan-Feb)

Bottom Line: Soluble and membrane fractions were obtained in phosphate saline (SS and SM) and Tris-HCl (TS and TM) from filariform larvae of S. venezuelensis.Ninety-two serum samples (n = 92) were obtained from 20 strongyloidiasis patients (Group I), 32 from patients with other parasitic diseases (Group II), and 40 from healthy individuals (Group III), and were analyzed by enzyme-linked immunosorbent assay (ELISA).Soluble fractions (SS and TS) showed 90.0% sensitivity and 88.9% specificity, whereas the membrane fractions (SM and TM) showed 95.0% sensitivity and 94.4% specificity.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil.

ABSTRACT
Strongyloides venezuelensis is a parasitic nematode of rodents frequently used to obtain heterologous antigens for the immunological diagnosis of human strongyloidiasis. The aim of this study was to evaluate membrane fractions from S. venezuelensis for human strongyloidiasis immunodiagnosis. Soluble and membrane fractions were obtained in phosphate saline (SS and SM) and Tris-HCl (TS and TM) from filariform larvae of S. venezuelensis. Ninety-two serum samples (n = 92) were obtained from 20 strongyloidiasis patients (Group I), 32 from patients with other parasitic diseases (Group II), and 40 from healthy individuals (Group III), and were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions (SS and TS) showed 90.0% sensitivity and 88.9% specificity, whereas the membrane fractions (SM and TM) showed 95.0% sensitivity and 94.4% specificity. The present results suggest the possible use of membrane fractions of S. venezuelensis as an alternative antigen for human strongyloidiasis immunodiagnosis.

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Related in: MedlinePlus

Electrophoretic profiles of antigenic fractions SS, SM and TS, TM, soluble andmembrane fractions from phosphate saline and Tris-HCl, respectively; 12% SDS-PAGEstained by silver nitrate. To each antigenic fraction were used 10 µg/mL.
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f01: Electrophoretic profiles of antigenic fractions SS, SM and TS, TM, soluble andmembrane fractions from phosphate saline and Tris-HCl, respectively; 12% SDS-PAGEstained by silver nitrate. To each antigenic fraction were used 10 µg/mL.

Mentions: The protein concentrations were 1.3-1.8 mg/mL and 6.2-6.9 mg/mL for soluble andmembrane fractions, respectively. Electrophoretical profiles of each antigenic fractionafter 12% SDS-PAGE are shown in Figure 1. Extractpreparations showed several proteic compounds with molecular weights ranging from < 15to 150 KDa. In membrane preparation, SM and TM fractions of 50, 70 and 120KDa wereevident.


Membrane fractions from Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis.

Corral MA, Paula FM, Gottardi M, Meisel DM, Chieffi PP, Gryschek RC - Rev. Inst. Med. Trop. Sao Paulo (2015 Jan-Feb)

Electrophoretic profiles of antigenic fractions SS, SM and TS, TM, soluble andmembrane fractions from phosphate saline and Tris-HCl, respectively; 12% SDS-PAGEstained by silver nitrate. To each antigenic fraction were used 10 µg/mL.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325527&req=5

f01: Electrophoretic profiles of antigenic fractions SS, SM and TS, TM, soluble andmembrane fractions from phosphate saline and Tris-HCl, respectively; 12% SDS-PAGEstained by silver nitrate. To each antigenic fraction were used 10 µg/mL.
Mentions: The protein concentrations were 1.3-1.8 mg/mL and 6.2-6.9 mg/mL for soluble andmembrane fractions, respectively. Electrophoretical profiles of each antigenic fractionafter 12% SDS-PAGE are shown in Figure 1. Extractpreparations showed several proteic compounds with molecular weights ranging from < 15to 150 KDa. In membrane preparation, SM and TM fractions of 50, 70 and 120KDa wereevident.

Bottom Line: Soluble and membrane fractions were obtained in phosphate saline (SS and SM) and Tris-HCl (TS and TM) from filariform larvae of S. venezuelensis.Ninety-two serum samples (n = 92) were obtained from 20 strongyloidiasis patients (Group I), 32 from patients with other parasitic diseases (Group II), and 40 from healthy individuals (Group III), and were analyzed by enzyme-linked immunosorbent assay (ELISA).Soluble fractions (SS and TS) showed 90.0% sensitivity and 88.9% specificity, whereas the membrane fractions (SM and TM) showed 95.0% sensitivity and 94.4% specificity.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil.

ABSTRACT
Strongyloides venezuelensis is a parasitic nematode of rodents frequently used to obtain heterologous antigens for the immunological diagnosis of human strongyloidiasis. The aim of this study was to evaluate membrane fractions from S. venezuelensis for human strongyloidiasis immunodiagnosis. Soluble and membrane fractions were obtained in phosphate saline (SS and SM) and Tris-HCl (TS and TM) from filariform larvae of S. venezuelensis. Ninety-two serum samples (n = 92) were obtained from 20 strongyloidiasis patients (Group I), 32 from patients with other parasitic diseases (Group II), and 40 from healthy individuals (Group III), and were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions (SS and TS) showed 90.0% sensitivity and 88.9% specificity, whereas the membrane fractions (SM and TM) showed 95.0% sensitivity and 94.4% specificity. The present results suggest the possible use of membrane fractions of S. venezuelensis as an alternative antigen for human strongyloidiasis immunodiagnosis.

Show MeSH
Related in: MedlinePlus