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Seropositivity for ascariosis and toxocariosis and cytokine expression among the indigenous people in the Venezuelan Delta region.

Araujo Z, Brandes S, Pinelli E, Bochichio MA, Palacios A, Wide A, Rivas-Santiago B, Jiménez JC - Rev. Inst. Med. Trop. Sao Paulo (2015 Jan-Feb)

Bottom Line: Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites.Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002).Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Inmunología de Enfermedades Infecciosas, Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela.

ABSTRACT
The present study aimed at measuring seropositivities for infection by Ascaris suum and Toxocara canis using the excretory/secretory (E/S) antigens from Ascaris suum (AES) and Toxocara canis (TES) within an indigenous population. In addition, quantification of cytokine expressions in peripheral blood cells was determined. A total of 50 Warao indigenous were included; of which 43 were adults and seven children. In adults, 44.1% were seropositive for both parasites; whereas children had only seropositivity to one or the other helminth. For ascariosis, the percentage of AES seropositivity in adults and children was high; 23.3% and 57.1%, respectively. While that for toxocariosis, the percentage of TES seropositivity in adults and children was low; 9.3% and 14.3%, respectively. The percentage of seronegativity was comparable for AES and TES antigens in adults (27.9%) and children (28.6%). When positive sera were analyzed by Western blotting technique using AES antigens; three bands of 97.2, 193.6 and 200.2 kDas were mostly recognized. When the TES antigens were used, nine major bands were mostly identified; 47.4, 52.2, 84.9, 98.2, 119.1, 131.3, 175.6, 184.4 and 193.6 kDas. Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites. Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002). Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

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Percentage of individuals showing positive expression of cytokine. Thepercentage of Warao indigenous with positive expression of cytokine: IL-2 (), IL-4 (), IL-6 (), IL-10 (), IL-12 (), IFN-γ(), TGF-β () and TNF-α (). (*)There was a significant increased expression of the IL-4in individuals with reactivity IgG to TES antigens (p <0.002).
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f05: Percentage of individuals showing positive expression of cytokine. Thepercentage of Warao indigenous with positive expression of cytokine: IL-2 (), IL-4 (), IL-6 (), IL-10 (), IL-12 (), IFN-γ(), TGF-β () and TNF-α (). (*)There was a significant increased expression of the IL-4in individuals with reactivity IgG to TES antigens (p <0.002).

Mentions: Cytokine expressions and seropositivity for Ascaris andToxocara.Figure 4 shows the cytokine expressions whenRT-PCR assays were used for the relative quantification of mRNA encoding for IL-2, IL-4,IL-6, IL-10, IL-12p35 (Fig. 4A), and IFN-γ, TGF-βand TNF-α (Fig. 4B) in peripheral blood cells fromWarao indigenous. Results indicated that the largest expression of transcript was forIFN-γ (100%) followed by TNF-α (90%), IL-2 (85%), IL-4 (67.5%), IL-12p35 (55%), TGF-β(50%), IL-6 and IL-10 (17.5%) (Fig. 5). When alogistic regression method was used to compare independent variables including cytokineexpression in peripheral blood cells from Warao indigenous that had or did not haveantibodies against the AES and TES antigens showed that there was only a significantincreased expression of the IL-4 in individuals with TES seropositivity(p < 0.002), whereas, the expression of all cytokines transcriptswere not different to that of individuals with AES seropositivity (Fig. 5).


Seropositivity for ascariosis and toxocariosis and cytokine expression among the indigenous people in the Venezuelan Delta region.

Araujo Z, Brandes S, Pinelli E, Bochichio MA, Palacios A, Wide A, Rivas-Santiago B, Jiménez JC - Rev. Inst. Med. Trop. Sao Paulo (2015 Jan-Feb)

Percentage of individuals showing positive expression of cytokine. Thepercentage of Warao indigenous with positive expression of cytokine: IL-2 (), IL-4 (), IL-6 (), IL-10 (), IL-12 (), IFN-γ(), TGF-β () and TNF-α (). (*)There was a significant increased expression of the IL-4in individuals with reactivity IgG to TES antigens (p <0.002).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325523&req=5

f05: Percentage of individuals showing positive expression of cytokine. Thepercentage of Warao indigenous with positive expression of cytokine: IL-2 (), IL-4 (), IL-6 (), IL-10 (), IL-12 (), IFN-γ(), TGF-β () and TNF-α (). (*)There was a significant increased expression of the IL-4in individuals with reactivity IgG to TES antigens (p <0.002).
Mentions: Cytokine expressions and seropositivity for Ascaris andToxocara.Figure 4 shows the cytokine expressions whenRT-PCR assays were used for the relative quantification of mRNA encoding for IL-2, IL-4,IL-6, IL-10, IL-12p35 (Fig. 4A), and IFN-γ, TGF-βand TNF-α (Fig. 4B) in peripheral blood cells fromWarao indigenous. Results indicated that the largest expression of transcript was forIFN-γ (100%) followed by TNF-α (90%), IL-2 (85%), IL-4 (67.5%), IL-12p35 (55%), TGF-β(50%), IL-6 and IL-10 (17.5%) (Fig. 5). When alogistic regression method was used to compare independent variables including cytokineexpression in peripheral blood cells from Warao indigenous that had or did not haveantibodies against the AES and TES antigens showed that there was only a significantincreased expression of the IL-4 in individuals with TES seropositivity(p < 0.002), whereas, the expression of all cytokines transcriptswere not different to that of individuals with AES seropositivity (Fig. 5).

Bottom Line: Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites.Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002).Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Inmunología de Enfermedades Infecciosas, Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela.

ABSTRACT
The present study aimed at measuring seropositivities for infection by Ascaris suum and Toxocara canis using the excretory/secretory (E/S) antigens from Ascaris suum (AES) and Toxocara canis (TES) within an indigenous population. In addition, quantification of cytokine expressions in peripheral blood cells was determined. A total of 50 Warao indigenous were included; of which 43 were adults and seven children. In adults, 44.1% were seropositive for both parasites; whereas children had only seropositivity to one or the other helminth. For ascariosis, the percentage of AES seropositivity in adults and children was high; 23.3% and 57.1%, respectively. While that for toxocariosis, the percentage of TES seropositivity in adults and children was low; 9.3% and 14.3%, respectively. The percentage of seronegativity was comparable for AES and TES antigens in adults (27.9%) and children (28.6%). When positive sera were analyzed by Western blotting technique using AES antigens; three bands of 97.2, 193.6 and 200.2 kDas were mostly recognized. When the TES antigens were used, nine major bands were mostly identified; 47.4, 52.2, 84.9, 98.2, 119.1, 131.3, 175.6, 184.4 and 193.6 kDas. Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites. Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002). Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

Show MeSH
Related in: MedlinePlus