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Seropositivity for ascariosis and toxocariosis and cytokine expression among the indigenous people in the Venezuelan Delta region.

Araujo Z, Brandes S, Pinelli E, Bochichio MA, Palacios A, Wide A, Rivas-Santiago B, Jiménez JC - Rev. Inst. Med. Trop. Sao Paulo (2015 Jan-Feb)

Bottom Line: Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites.Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002).Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Inmunología de Enfermedades Infecciosas, Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela.

ABSTRACT
The present study aimed at measuring seropositivities for infection by Ascaris suum and Toxocara canis using the excretory/secretory (E/S) antigens from Ascaris suum (AES) and Toxocara canis (TES) within an indigenous population. In addition, quantification of cytokine expressions in peripheral blood cells was determined. A total of 50 Warao indigenous were included; of which 43 were adults and seven children. In adults, 44.1% were seropositive for both parasites; whereas children had only seropositivity to one or the other helminth. For ascariosis, the percentage of AES seropositivity in adults and children was high; 23.3% and 57.1%, respectively. While that for toxocariosis, the percentage of TES seropositivity in adults and children was low; 9.3% and 14.3%, respectively. The percentage of seronegativity was comparable for AES and TES antigens in adults (27.9%) and children (28.6%). When positive sera were analyzed by Western blotting technique using AES antigens; three bands of 97.2, 193.6 and 200.2 kDas were mostly recognized. When the TES antigens were used, nine major bands were mostly identified; 47.4, 52.2, 84.9, 98.2, 119.1, 131.3, 175.6, 184.4 and 193.6 kDas. Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites. Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002). Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

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Western blotting of Toxocara canis antigens. Westernblotting procedures showing some of the most frequently obtained bandsaccording to TES seropositivity (3A). Tested sera were assayed in order toidentify the bands of T. canis. WM: Molecular weight marker.Band (3A). Percentage of individuals who composed the groups according toanti-TES IgG antibodies (3B): Group of bands lower than 50 kDa (), group of bands between 50 and 100 kDa (), group of bands between 101-150 kDa (), and group of bands between 151- 207 kDa ().
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f03: Western blotting of Toxocara canis antigens. Westernblotting procedures showing some of the most frequently obtained bandsaccording to TES seropositivity (3A). Tested sera were assayed in order toidentify the bands of T. canis. WM: Molecular weight marker.Band (3A). Percentage of individuals who composed the groups according toanti-TES IgG antibodies (3B): Group of bands lower than 50 kDa (), group of bands between 50 and 100 kDa (), group of bands between 101-150 kDa (), and group of bands between 151- 207 kDa ().

Mentions: As regards T. canis, the immunoblotting showed a pattern ofbands, which was ranging from 22.9 kDa to 223 kDas; among these 11 major bands wereprincipally identified; 84.9 kDa (37.7%), 52.2 kDa (33.3%), 131.3 kDa (31.1%), 98.2 kDa(28.8%), 119.1 kDa (26.6%), 41 kDa (26.2%), 184.4 kDa (24.4%), 175.6 and 47.4 kDas(22.2%), 193.6 kDa (15.5%) and 50 kDa (11.9%). Sera from healthy individuals did notshow any reactivity (data not shown). Western blotting procedures showing the obtainedbands and the WB profile according to TES seropositivity are shown in Figure 3. Western blotting analysis using TESantigens showed the TES pattern composed by bands ranging from 22.9 kDa to 223 kDa (someof the most frequently recognized bands are shown in Figure 3A). The WB profile according to TES seropositivity could be dividedinto the following groups: the group composed of bands lower than 50 kDa (42.1%) was themost reactive; followed by the group of bands between 50 kDa and 100 kDa (28%), 151 kDaand 207 kDa (18.4%) and 101-150 kDas (11.4%) (Fig.3B).


Seropositivity for ascariosis and toxocariosis and cytokine expression among the indigenous people in the Venezuelan Delta region.

Araujo Z, Brandes S, Pinelli E, Bochichio MA, Palacios A, Wide A, Rivas-Santiago B, Jiménez JC - Rev. Inst. Med. Trop. Sao Paulo (2015 Jan-Feb)

Western blotting of Toxocara canis antigens. Westernblotting procedures showing some of the most frequently obtained bandsaccording to TES seropositivity (3A). Tested sera were assayed in order toidentify the bands of T. canis. WM: Molecular weight marker.Band (3A). Percentage of individuals who composed the groups according toanti-TES IgG antibodies (3B): Group of bands lower than 50 kDa (), group of bands between 50 and 100 kDa (), group of bands between 101-150 kDa (), and group of bands between 151- 207 kDa ().
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325523&req=5

f03: Western blotting of Toxocara canis antigens. Westernblotting procedures showing some of the most frequently obtained bandsaccording to TES seropositivity (3A). Tested sera were assayed in order toidentify the bands of T. canis. WM: Molecular weight marker.Band (3A). Percentage of individuals who composed the groups according toanti-TES IgG antibodies (3B): Group of bands lower than 50 kDa (), group of bands between 50 and 100 kDa (), group of bands between 101-150 kDa (), and group of bands between 151- 207 kDa ().
Mentions: As regards T. canis, the immunoblotting showed a pattern ofbands, which was ranging from 22.9 kDa to 223 kDas; among these 11 major bands wereprincipally identified; 84.9 kDa (37.7%), 52.2 kDa (33.3%), 131.3 kDa (31.1%), 98.2 kDa(28.8%), 119.1 kDa (26.6%), 41 kDa (26.2%), 184.4 kDa (24.4%), 175.6 and 47.4 kDas(22.2%), 193.6 kDa (15.5%) and 50 kDa (11.9%). Sera from healthy individuals did notshow any reactivity (data not shown). Western blotting procedures showing the obtainedbands and the WB profile according to TES seropositivity are shown in Figure 3. Western blotting analysis using TESantigens showed the TES pattern composed by bands ranging from 22.9 kDa to 223 kDa (someof the most frequently recognized bands are shown in Figure 3A). The WB profile according to TES seropositivity could be dividedinto the following groups: the group composed of bands lower than 50 kDa (42.1%) was themost reactive; followed by the group of bands between 50 kDa and 100 kDa (28%), 151 kDaand 207 kDa (18.4%) and 101-150 kDas (11.4%) (Fig.3B).

Bottom Line: Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites.Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002).Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Inmunología de Enfermedades Infecciosas, Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela.

ABSTRACT
The present study aimed at measuring seropositivities for infection by Ascaris suum and Toxocara canis using the excretory/secretory (E/S) antigens from Ascaris suum (AES) and Toxocara canis (TES) within an indigenous population. In addition, quantification of cytokine expressions in peripheral blood cells was determined. A total of 50 Warao indigenous were included; of which 43 were adults and seven children. In adults, 44.1% were seropositive for both parasites; whereas children had only seropositivity to one or the other helminth. For ascariosis, the percentage of AES seropositivity in adults and children was high; 23.3% and 57.1%, respectively. While that for toxocariosis, the percentage of TES seropositivity in adults and children was low; 9.3% and 14.3%, respectively. The percentage of seronegativity was comparable for AES and TES antigens in adults (27.9%) and children (28.6%). When positive sera were analyzed by Western blotting technique using AES antigens; three bands of 97.2, 193.6 and 200.2 kDas were mostly recognized. When the TES antigens were used, nine major bands were mostly identified; 47.4, 52.2, 84.9, 98.2, 119.1, 131.3, 175.6, 184.4 and 193.6 kDas. Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites. Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002). Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

Show MeSH
Related in: MedlinePlus