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Seropositivity for ascariosis and toxocariosis and cytokine expression among the indigenous people in the Venezuelan Delta region.

Araujo Z, Brandes S, Pinelli E, Bochichio MA, Palacios A, Wide A, Rivas-Santiago B, Jiménez JC - Rev. Inst. Med. Trop. Sao Paulo (2015 Jan-Feb)

Bottom Line: Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites.Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002).Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Inmunología de Enfermedades Infecciosas, Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela.

ABSTRACT
The present study aimed at measuring seropositivities for infection by Ascaris suum and Toxocara canis using the excretory/secretory (E/S) antigens from Ascaris suum (AES) and Toxocara canis (TES) within an indigenous population. In addition, quantification of cytokine expressions in peripheral blood cells was determined. A total of 50 Warao indigenous were included; of which 43 were adults and seven children. In adults, 44.1% were seropositive for both parasites; whereas children had only seropositivity to one or the other helminth. For ascariosis, the percentage of AES seropositivity in adults and children was high; 23.3% and 57.1%, respectively. While that for toxocariosis, the percentage of TES seropositivity in adults and children was low; 9.3% and 14.3%, respectively. The percentage of seronegativity was comparable for AES and TES antigens in adults (27.9%) and children (28.6%). When positive sera were analyzed by Western blotting technique using AES antigens; three bands of 97.2, 193.6 and 200.2 kDas were mostly recognized. When the TES antigens were used, nine major bands were mostly identified; 47.4, 52.2, 84.9, 98.2, 119.1, 131.3, 175.6, 184.4 and 193.6 kDas. Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites. Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002). Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

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Related in: MedlinePlus

Western blotting of Ascaris suum antigens. Westernblotting procedures showing some of the most frequently recognized bandsobtained according to AES seropositivity (2A). Tested sera were assayed inorder to identify the bands of A. suum. WM: Molecular weightmarker (2A). Percentage of individuals who composed the groups of bandsaccording to anti-AES IgG antibodies (2B): Group of bands lower than 50 kDa (), group of bands between 50 and 100 kDa (), group of bands between 101-150 kDa (), and group of bands between 151- 207 kDa ().
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f02: Western blotting of Ascaris suum antigens. Westernblotting procedures showing some of the most frequently recognized bandsobtained according to AES seropositivity (2A). Tested sera were assayed inorder to identify the bands of A. suum. WM: Molecular weightmarker (2A). Percentage of individuals who composed the groups of bandsaccording to anti-AES IgG antibodies (2B): Group of bands lower than 50 kDa (), group of bands between 50 and 100 kDa (), group of bands between 101-150 kDa (), and group of bands between 151- 207 kDa ().

Mentions: Western blotting and band patterns. In order to identify the pattern ofbands displayed by sera from Warao; AES and TES antigens were analyzed by Westernblotting technique. For A. suum, the immunoblotting showed a pattern ofbands ranging from 52 to 206.9 kDas, 12 bands of 206.9, 200.2, 193.6, 149, 114.6, 97.2,94.1, 82.5, 67.8, 65.6, 55.6 and 52.2, kDas from A. suum were detected;of these, three bands of 193.6 kDa (33.3%), 200.2 kDa and 97.2 kDa (22.2%) wereprincipally recognized. Sera from healthy individuals did not show any IgG reactivity(data not shown). Western blotting procedures showing the obtained bands and the WBprofile according to AES seropositivity are shown in Figure 2. Western blotting analysis using AES antigens showed the AES patterncomposed by bands ranging from 52 kDa to 206.9 kDa (some of the most frequentlyrecognized bands are shown in Figure 2A). The WBprofile could be divided in the following groups according IgG reactivity to AESantigens; the group composed of bands between 50 kDa and 100 kDa was the most reactive(50%), followed by 151-207 kDas (37.5%) and 101-150 kDas (12.5%) (Fig. 2B).


Seropositivity for ascariosis and toxocariosis and cytokine expression among the indigenous people in the Venezuelan Delta region.

Araujo Z, Brandes S, Pinelli E, Bochichio MA, Palacios A, Wide A, Rivas-Santiago B, Jiménez JC - Rev. Inst. Med. Trop. Sao Paulo (2015 Jan-Feb)

Western blotting of Ascaris suum antigens. Westernblotting procedures showing some of the most frequently recognized bandsobtained according to AES seropositivity (2A). Tested sera were assayed inorder to identify the bands of A. suum. WM: Molecular weightmarker (2A). Percentage of individuals who composed the groups of bandsaccording to anti-AES IgG antibodies (2B): Group of bands lower than 50 kDa (), group of bands between 50 and 100 kDa (), group of bands between 101-150 kDa (), and group of bands between 151- 207 kDa ().
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325523&req=5

f02: Western blotting of Ascaris suum antigens. Westernblotting procedures showing some of the most frequently recognized bandsobtained according to AES seropositivity (2A). Tested sera were assayed inorder to identify the bands of A. suum. WM: Molecular weightmarker (2A). Percentage of individuals who composed the groups of bandsaccording to anti-AES IgG antibodies (2B): Group of bands lower than 50 kDa (), group of bands between 50 and 100 kDa (), group of bands between 101-150 kDa (), and group of bands between 151- 207 kDa ().
Mentions: Western blotting and band patterns. In order to identify the pattern ofbands displayed by sera from Warao; AES and TES antigens were analyzed by Westernblotting technique. For A. suum, the immunoblotting showed a pattern ofbands ranging from 52 to 206.9 kDas, 12 bands of 206.9, 200.2, 193.6, 149, 114.6, 97.2,94.1, 82.5, 67.8, 65.6, 55.6 and 52.2, kDas from A. suum were detected;of these, three bands of 193.6 kDa (33.3%), 200.2 kDa and 97.2 kDa (22.2%) wereprincipally recognized. Sera from healthy individuals did not show any IgG reactivity(data not shown). Western blotting procedures showing the obtained bands and the WBprofile according to AES seropositivity are shown in Figure 2. Western blotting analysis using AES antigens showed the AES patterncomposed by bands ranging from 52 kDa to 206.9 kDa (some of the most frequentlyrecognized bands are shown in Figure 2A). The WBprofile could be divided in the following groups according IgG reactivity to AESantigens; the group composed of bands between 50 kDa and 100 kDa was the most reactive(50%), followed by 151-207 kDas (37.5%) and 101-150 kDas (12.5%) (Fig. 2B).

Bottom Line: Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites.Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002).Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Inmunología de Enfermedades Infecciosas, Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela.

ABSTRACT
The present study aimed at measuring seropositivities for infection by Ascaris suum and Toxocara canis using the excretory/secretory (E/S) antigens from Ascaris suum (AES) and Toxocara canis (TES) within an indigenous population. In addition, quantification of cytokine expressions in peripheral blood cells was determined. A total of 50 Warao indigenous were included; of which 43 were adults and seven children. In adults, 44.1% were seropositive for both parasites; whereas children had only seropositivity to one or the other helminth. For ascariosis, the percentage of AES seropositivity in adults and children was high; 23.3% and 57.1%, respectively. While that for toxocariosis, the percentage of TES seropositivity in adults and children was low; 9.3% and 14.3%, respectively. The percentage of seronegativity was comparable for AES and TES antigens in adults (27.9%) and children (28.6%). When positive sera were analyzed by Western blotting technique using AES antigens; three bands of 97.2, 193.6 and 200.2 kDas were mostly recognized. When the TES antigens were used, nine major bands were mostly identified; 47.4, 52.2, 84.9, 98.2, 119.1, 131.3, 175.6, 184.4 and 193.6 kDas. Stool examinations showed that Blastocystis hominis, Hymenolepis nana and Entamoeba coli were the most commonly observed intestinal parasites. Quantification of cytokines IFN-γ, IL-2, IL-6, TGF-β, TNF-α, IL-10 and IL-4 expressions showed that there was only a significant increased expression of IL-4 in indigenous with TES seropositivity (p < 0.002). Ascaris and Toxocara seropositivity was prevalent among Warao indigenous.

Show MeSH
Related in: MedlinePlus