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Spinal fusion with demineralized calf fetal growth plate as novel biomaterial in rat model: a preliminary study.

Bigham-Sadegh A, Karimi I, Oryan A, Mahmoudi E, Shafiei-Sarvestani Z - Int J Spine Surg (2014)

Bottom Line: In group II (n = 8) commercial DBM was placed in the same manner.The manual palpation, radiological, gross and histopathological findings indicate high potential of the DCFGP in spinal fusion.The newly formed bone tissue was observed in all implanted areas on the 42nd day after operation in the two groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

ABSTRACT

Background: Spinal fusions are being performed for various pathologies of the spine such as degenerative diseases, deformities, tumors and fractures. Recently, other bone substitutes such as demineralized bone matrix (DBM) have been developed for spinal fusion. Therefore, this study was conducted to evaluate the intertransverse posterolateral fusion with the Bovine fetal growth plate (DCFGP) and compare it with commercial DBM in rat model.

Methods: A total of 16 mature male rats (aged 4 months and weighing 200-300 g) were randomly divided in two groups. After a skin incision on posterolateral site, two separate fascial incisions were made 3 mm from the midline. A muscle-splitting approach was used to expose the transverse processes of L4 and L5. Group I (n = 8) underwent with implanted Bovine fetal growth plate among decorticated transverse processes. In group II (n = 8) commercial DBM was placed in the same manner. Fusion was evaluated by manual palpation, radiographical, gross and histopathological analysis.

Results: The manual palpation, radiological, gross and histopathological findings indicate high potential of the DCFGP in spinal fusion. At the 42nd postoperative day, new bone formation as evidenced by a bridge between L4 and L5 was visualized in all rats implanted with DCFGP and commercial DBM. The newly formed bone tissue was observed in all implanted areas on the 42nd day after operation in the two groups.

Conclusions: The spinal fusion of the animals of both groups demonstrated more advanced osteogenic potential and resulted in proper fusion of the transverse process of lumbar vertebra.

No MeSH data available.


Related in: MedlinePlus

Histology of 6-week samples of fusion by DCFGP (A and B) and commercial DBM (C and D). Immature and woven bone in DCFGP (A, 10X H & E Staining). Trabecular bone (white arrow) and marrow formation (black arrow) are seen in the fusion area of the same figure in a high magnification view (B, 40X H&E Staining). More mature bone (black right angle) with osteocyte cells are seen in the lesion of the commercial DBM group (C, 10X H & E Staining). Higher power view of the same picture shows bony tissue (white arrow) and marrow formation (black arrow) (D, 40X H&E Staining)
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Figure 0002: Histology of 6-week samples of fusion by DCFGP (A and B) and commercial DBM (C and D). Immature and woven bone in DCFGP (A, 10X H & E Staining). Trabecular bone (white arrow) and marrow formation (black arrow) are seen in the fusion area of the same figure in a high magnification view (B, 40X H&E Staining). More mature bone (black right angle) with osteocyte cells are seen in the lesion of the commercial DBM group (C, 10X H & E Staining). Higher power view of the same picture shows bony tissue (white arrow) and marrow formation (black arrow) (D, 40X H&E Staining)

Mentions: Staining of the specimens with hemotoxylin and eosin revealed that intertransverse process implantation of the DCFGP and commercial DBM lead to fibrocartilaginous response with apparent bone formation without any inflammatory response. The newly formed bone tissue was observed in all implanted areas on the 42nd day after operation in the two groups. Bone tissue which had an optimum bone formation score was observed on the 42nd postoperative day (Figure 1, Table 1). Differentiation of the cartilaginous tissue to a mineralized foci in this highly vascular area resulted to a bony bridge architecture and formation of an ossicle filled with bone marrow elements (day 42) (Figure 2).


Spinal fusion with demineralized calf fetal growth plate as novel biomaterial in rat model: a preliminary study.

Bigham-Sadegh A, Karimi I, Oryan A, Mahmoudi E, Shafiei-Sarvestani Z - Int J Spine Surg (2014)

Histology of 6-week samples of fusion by DCFGP (A and B) and commercial DBM (C and D). Immature and woven bone in DCFGP (A, 10X H & E Staining). Trabecular bone (white arrow) and marrow formation (black arrow) are seen in the fusion area of the same figure in a high magnification view (B, 40X H&E Staining). More mature bone (black right angle) with osteocyte cells are seen in the lesion of the commercial DBM group (C, 10X H & E Staining). Higher power view of the same picture shows bony tissue (white arrow) and marrow formation (black arrow) (D, 40X H&E Staining)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325481&req=5

Figure 0002: Histology of 6-week samples of fusion by DCFGP (A and B) and commercial DBM (C and D). Immature and woven bone in DCFGP (A, 10X H & E Staining). Trabecular bone (white arrow) and marrow formation (black arrow) are seen in the fusion area of the same figure in a high magnification view (B, 40X H&E Staining). More mature bone (black right angle) with osteocyte cells are seen in the lesion of the commercial DBM group (C, 10X H & E Staining). Higher power view of the same picture shows bony tissue (white arrow) and marrow formation (black arrow) (D, 40X H&E Staining)
Mentions: Staining of the specimens with hemotoxylin and eosin revealed that intertransverse process implantation of the DCFGP and commercial DBM lead to fibrocartilaginous response with apparent bone formation without any inflammatory response. The newly formed bone tissue was observed in all implanted areas on the 42nd day after operation in the two groups. Bone tissue which had an optimum bone formation score was observed on the 42nd postoperative day (Figure 1, Table 1). Differentiation of the cartilaginous tissue to a mineralized foci in this highly vascular area resulted to a bony bridge architecture and formation of an ossicle filled with bone marrow elements (day 42) (Figure 2).

Bottom Line: In group II (n = 8) commercial DBM was placed in the same manner.The manual palpation, radiological, gross and histopathological findings indicate high potential of the DCFGP in spinal fusion.The newly formed bone tissue was observed in all implanted areas on the 42nd day after operation in the two groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

ABSTRACT

Background: Spinal fusions are being performed for various pathologies of the spine such as degenerative diseases, deformities, tumors and fractures. Recently, other bone substitutes such as demineralized bone matrix (DBM) have been developed for spinal fusion. Therefore, this study was conducted to evaluate the intertransverse posterolateral fusion with the Bovine fetal growth plate (DCFGP) and compare it with commercial DBM in rat model.

Methods: A total of 16 mature male rats (aged 4 months and weighing 200-300 g) were randomly divided in two groups. After a skin incision on posterolateral site, two separate fascial incisions were made 3 mm from the midline. A muscle-splitting approach was used to expose the transverse processes of L4 and L5. Group I (n = 8) underwent with implanted Bovine fetal growth plate among decorticated transverse processes. In group II (n = 8) commercial DBM was placed in the same manner. Fusion was evaluated by manual palpation, radiographical, gross and histopathological analysis.

Results: The manual palpation, radiological, gross and histopathological findings indicate high potential of the DCFGP in spinal fusion. At the 42nd postoperative day, new bone formation as evidenced by a bridge between L4 and L5 was visualized in all rats implanted with DCFGP and commercial DBM. The newly formed bone tissue was observed in all implanted areas on the 42nd day after operation in the two groups.

Conclusions: The spinal fusion of the animals of both groups demonstrated more advanced osteogenic potential and resulted in proper fusion of the transverse process of lumbar vertebra.

No MeSH data available.


Related in: MedlinePlus