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Spinal fusion with demineralized calf fetal growth plate as novel biomaterial in rat model: a preliminary study.

Bigham-Sadegh A, Karimi I, Oryan A, Mahmoudi E, Shafiei-Sarvestani Z - Int J Spine Surg (2014)

Bottom Line: In group II (n = 8) commercial DBM was placed in the same manner.The manual palpation, radiological, gross and histopathological findings indicate high potential of the DCFGP in spinal fusion.The newly formed bone tissue was observed in all implanted areas on the 42nd day after operation in the two groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

ABSTRACT

Background: Spinal fusions are being performed for various pathologies of the spine such as degenerative diseases, deformities, tumors and fractures. Recently, other bone substitutes such as demineralized bone matrix (DBM) have been developed for spinal fusion. Therefore, this study was conducted to evaluate the intertransverse posterolateral fusion with the Bovine fetal growth plate (DCFGP) and compare it with commercial DBM in rat model.

Methods: A total of 16 mature male rats (aged 4 months and weighing 200-300 g) were randomly divided in two groups. After a skin incision on posterolateral site, two separate fascial incisions were made 3 mm from the midline. A muscle-splitting approach was used to expose the transverse processes of L4 and L5. Group I (n = 8) underwent with implanted Bovine fetal growth plate among decorticated transverse processes. In group II (n = 8) commercial DBM was placed in the same manner. Fusion was evaluated by manual palpation, radiographical, gross and histopathological analysis.

Results: The manual palpation, radiological, gross and histopathological findings indicate high potential of the DCFGP in spinal fusion. At the 42nd postoperative day, new bone formation as evidenced by a bridge between L4 and L5 was visualized in all rats implanted with DCFGP and commercial DBM. The newly formed bone tissue was observed in all implanted areas on the 42nd day after operation in the two groups.

Conclusions: The spinal fusion of the animals of both groups demonstrated more advanced osteogenic potential and resulted in proper fusion of the transverse process of lumbar vertebra.

No MeSH data available.


Related in: MedlinePlus

Radiographic evaluation on the 14th (A and B), 28th (C and D) and 42nd (E and F) postoperative days. Samples of DCFGP treated spine with fusion (A, C and E) and commercial treated sample (B, D, and F). The red arrows identify the radiopaque tissue masses on both sides of spine at the L4 and L5 segments.
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Figure 0001: Radiographic evaluation on the 14th (A and B), 28th (C and D) and 42nd (E and F) postoperative days. Samples of DCFGP treated spine with fusion (A, C and E) and commercial treated sample (B, D, and F). The red arrows identify the radiopaque tissue masses on both sides of spine at the L4 and L5 segments.

Mentions: There was no intraoperative and postoperative death during the study. None of the rats sustained a wound infection or surgery complication. The radiographs did not show significant differences between the two groups at 14th, 28th and 42nd postoperative days. The radio-opacity of the implanted area showed gradual increasing from 14th, to 42nd postoperative days (Table 1). At the 42nd postoperative day, new bone formation as evidenced by a bridge between L4 and L5 was visualized in all rats implanted with DCFGP and commercial DBM. An increased radio-opacity with discrete foci of densities and imparting a granular appearance to the images was observed in in the injured areas of the animals of the two groups on the 42nd postoperative day. Radiographs also showed that there is excess bone formation at more than the single L4-5 operated level (Figure 1).


Spinal fusion with demineralized calf fetal growth plate as novel biomaterial in rat model: a preliminary study.

Bigham-Sadegh A, Karimi I, Oryan A, Mahmoudi E, Shafiei-Sarvestani Z - Int J Spine Surg (2014)

Radiographic evaluation on the 14th (A and B), 28th (C and D) and 42nd (E and F) postoperative days. Samples of DCFGP treated spine with fusion (A, C and E) and commercial treated sample (B, D, and F). The red arrows identify the radiopaque tissue masses on both sides of spine at the L4 and L5 segments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325481&req=5

Figure 0001: Radiographic evaluation on the 14th (A and B), 28th (C and D) and 42nd (E and F) postoperative days. Samples of DCFGP treated spine with fusion (A, C and E) and commercial treated sample (B, D, and F). The red arrows identify the radiopaque tissue masses on both sides of spine at the L4 and L5 segments.
Mentions: There was no intraoperative and postoperative death during the study. None of the rats sustained a wound infection or surgery complication. The radiographs did not show significant differences between the two groups at 14th, 28th and 42nd postoperative days. The radio-opacity of the implanted area showed gradual increasing from 14th, to 42nd postoperative days (Table 1). At the 42nd postoperative day, new bone formation as evidenced by a bridge between L4 and L5 was visualized in all rats implanted with DCFGP and commercial DBM. An increased radio-opacity with discrete foci of densities and imparting a granular appearance to the images was observed in in the injured areas of the animals of the two groups on the 42nd postoperative day. Radiographs also showed that there is excess bone formation at more than the single L4-5 operated level (Figure 1).

Bottom Line: In group II (n = 8) commercial DBM was placed in the same manner.The manual palpation, radiological, gross and histopathological findings indicate high potential of the DCFGP in spinal fusion.The newly formed bone tissue was observed in all implanted areas on the 42nd day after operation in the two groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

ABSTRACT

Background: Spinal fusions are being performed for various pathologies of the spine such as degenerative diseases, deformities, tumors and fractures. Recently, other bone substitutes such as demineralized bone matrix (DBM) have been developed for spinal fusion. Therefore, this study was conducted to evaluate the intertransverse posterolateral fusion with the Bovine fetal growth plate (DCFGP) and compare it with commercial DBM in rat model.

Methods: A total of 16 mature male rats (aged 4 months and weighing 200-300 g) were randomly divided in two groups. After a skin incision on posterolateral site, two separate fascial incisions were made 3 mm from the midline. A muscle-splitting approach was used to expose the transverse processes of L4 and L5. Group I (n = 8) underwent with implanted Bovine fetal growth plate among decorticated transverse processes. In group II (n = 8) commercial DBM was placed in the same manner. Fusion was evaluated by manual palpation, radiographical, gross and histopathological analysis.

Results: The manual palpation, radiological, gross and histopathological findings indicate high potential of the DCFGP in spinal fusion. At the 42nd postoperative day, new bone formation as evidenced by a bridge between L4 and L5 was visualized in all rats implanted with DCFGP and commercial DBM. The newly formed bone tissue was observed in all implanted areas on the 42nd day after operation in the two groups.

Conclusions: The spinal fusion of the animals of both groups demonstrated more advanced osteogenic potential and resulted in proper fusion of the transverse process of lumbar vertebra.

No MeSH data available.


Related in: MedlinePlus