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Molecular characterization of Aspergillus infections in an Iranian educational hospital using RAPD-PCR method.

Diba K, Makhdoomi K, Mirhendi H - Iran J Basic Med Sci (2014)

Bottom Line: The nosocomial infections by Aspergillus species are associated with constructions and increased dust loads in hospital indoors.In the case of matched pairs, Aspergillus niger and A. flavus isolated from broncoalveolar lavage and sinus discharge were relevant to those of air conditioner and walls surfaces, respectively.The hospital sources for the Aspergillus clinical isolates included air condition and walls.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.

ABSTRACT

Objectives: The nosocomial infections by Aspergillus species are associated with constructions and increased dust loads in hospital indoors. Our main object was to find the environmental sources of Aspergillus species causing hospital acquired infections.

Materials and methods: The clinical and environmental samplings were performed during 18 months from spring 2010 to summer 2011 in Imam educational hospital, Urmia, Iran. A morphological diagnosis was performed including microscopic characterization of isolated aspergillus from cultured specimens and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) for the identification in the level of species. Random amplified polymorphic DNA - PCR RAPD-PCR using random primers for rDNA gene was performed to compare Aspergillus isolates of clinical cases with the relevant environmental sources.

Results: Use of RAPD method resulted various differential patterns, so that some Aspergillus isolates from the clinical and hospital indoor were completely matched (matched pairs) and some other Aspergillus isolates were not matched. In the case of matched pairs, Aspergillus niger and A. flavus isolated from broncoalveolar lavage and sinus discharge were relevant to those of air conditioner and walls surfaces, respectively.

Conclusion: The hospital sources for the Aspergillus clinical isolates included air condition and walls. RAPD-PCR analysis can play a trivial role to find the hospital sources of Aspergillus clinical isolates.

No MeSH data available.


Related in: MedlinePlus

RAPD patterns of clinical and environmental for the strains 32 and 45 are similar using each tested primers (a, c). Application of P1 in RAPD resulted different patterns for the pairs of 16, 36 and 37 (b). P2 resulted same patterns for Aspergillus groups 16, 31 and 37 (d)
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Figure 1: RAPD patterns of clinical and environmental for the strains 32 and 45 are similar using each tested primers (a, c). Application of P1 in RAPD resulted different patterns for the pairs of 16, 36 and 37 (b). P2 resulted same patterns for Aspergillus groups 16, 31 and 37 (d)

Mentions: From 65 clinical and environmental isolates of Aspergillus species, 28 isolates were common between cases and their environments in the level of species. For making an exact correlation among the clinical and environmental isolates, the findings of RAPD-PCR were analyzed. The random primers, P1-P6 made different electrophoretic DNA patterns for most cases. For example, application of P1 resulted in different DNA patterns for the pairs (clinical and environmental isolates) 16, 36 and 37 from 65 cases of Aspergillosis. Just in one case (pair 31) same DNA patterns were observed (Figure 1c). Use of primer P2 resulted in identical patterns for three pairs: 16, 31 and 37 as shown in Figure 1 d. At the same way, the other primers created common patterns not more than one. Comparison of clinical and environmental pairs with RAPD-PCR using six random primers revealed similar electrophoretic DNA patterns for clinical and environmental Aspergillus isolates (Figures 1 a, b) just in two of 28 pairs including 32 and 45. A. niger and A. flavus were included in the pairs 32 and 45 respectively. The Aspergillus isolate of bronco alveolar lavage of case (32) was completely similar to that of relevant air conditioner used in the private room. Also, DNA pattern of A. flavus isolated from sinus discharge of case (45) was similar to the isolate of wall swabs. Other Aspergillus pairs showed no similarity (Table 3).


Molecular characterization of Aspergillus infections in an Iranian educational hospital using RAPD-PCR method.

Diba K, Makhdoomi K, Mirhendi H - Iran J Basic Med Sci (2014)

RAPD patterns of clinical and environmental for the strains 32 and 45 are similar using each tested primers (a, c). Application of P1 in RAPD resulted different patterns for the pairs of 16, 36 and 37 (b). P2 resulted same patterns for Aspergillus groups 16, 31 and 37 (d)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4322146&req=5

Figure 1: RAPD patterns of clinical and environmental for the strains 32 and 45 are similar using each tested primers (a, c). Application of P1 in RAPD resulted different patterns for the pairs of 16, 36 and 37 (b). P2 resulted same patterns for Aspergillus groups 16, 31 and 37 (d)
Mentions: From 65 clinical and environmental isolates of Aspergillus species, 28 isolates were common between cases and their environments in the level of species. For making an exact correlation among the clinical and environmental isolates, the findings of RAPD-PCR were analyzed. The random primers, P1-P6 made different electrophoretic DNA patterns for most cases. For example, application of P1 resulted in different DNA patterns for the pairs (clinical and environmental isolates) 16, 36 and 37 from 65 cases of Aspergillosis. Just in one case (pair 31) same DNA patterns were observed (Figure 1c). Use of primer P2 resulted in identical patterns for three pairs: 16, 31 and 37 as shown in Figure 1 d. At the same way, the other primers created common patterns not more than one. Comparison of clinical and environmental pairs with RAPD-PCR using six random primers revealed similar electrophoretic DNA patterns for clinical and environmental Aspergillus isolates (Figures 1 a, b) just in two of 28 pairs including 32 and 45. A. niger and A. flavus were included in the pairs 32 and 45 respectively. The Aspergillus isolate of bronco alveolar lavage of case (32) was completely similar to that of relevant air conditioner used in the private room. Also, DNA pattern of A. flavus isolated from sinus discharge of case (45) was similar to the isolate of wall swabs. Other Aspergillus pairs showed no similarity (Table 3).

Bottom Line: The nosocomial infections by Aspergillus species are associated with constructions and increased dust loads in hospital indoors.In the case of matched pairs, Aspergillus niger and A. flavus isolated from broncoalveolar lavage and sinus discharge were relevant to those of air conditioner and walls surfaces, respectively.The hospital sources for the Aspergillus clinical isolates included air condition and walls.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.

ABSTRACT

Objectives: The nosocomial infections by Aspergillus species are associated with constructions and increased dust loads in hospital indoors. Our main object was to find the environmental sources of Aspergillus species causing hospital acquired infections.

Materials and methods: The clinical and environmental samplings were performed during 18 months from spring 2010 to summer 2011 in Imam educational hospital, Urmia, Iran. A morphological diagnosis was performed including microscopic characterization of isolated aspergillus from cultured specimens and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) for the identification in the level of species. Random amplified polymorphic DNA - PCR RAPD-PCR using random primers for rDNA gene was performed to compare Aspergillus isolates of clinical cases with the relevant environmental sources.

Results: Use of RAPD method resulted various differential patterns, so that some Aspergillus isolates from the clinical and hospital indoor were completely matched (matched pairs) and some other Aspergillus isolates were not matched. In the case of matched pairs, Aspergillus niger and A. flavus isolated from broncoalveolar lavage and sinus discharge were relevant to those of air conditioner and walls surfaces, respectively.

Conclusion: The hospital sources for the Aspergillus clinical isolates included air condition and walls. RAPD-PCR analysis can play a trivial role to find the hospital sources of Aspergillus clinical isolates.

No MeSH data available.


Related in: MedlinePlus