Limits...
New insight into the immunomodulatory mechanisms of Tretinoin in NMRI mice.

Froushani SM, Galeh HE - Iran J Basic Med Sci (2014)

Bottom Line: Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10.However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: Recent evidence have proposed that Tretinoin produced in the gut preferentially promote differentiation of FoxP3+Treg cells but inhibits Th17 lymphocytes, and this may be the main immunomdulatory mechanism of Tretinoin in vivo. This study was done to investigate the effects of Tretinoin in outbred white mice after challenge with sheep red blood cells (SRBC).

Materials and methods: Twenty male NMRI-mice randomly allocated in two equal groups. Mice were treated with 1×10(9) SRBCs emulsified in CFA intraperitoneally twice with one weak interval. Animals were bled 5 days after last injection. Moreover, 48 hr before bleeding time, 1×10(9) SRBCs were injected into the left hind foot pad of mice. Tretinoin (25 mg/kg-every other day) were intraperitoneally injected into the treatment group from the beginning of the study and continued throughout the study. The levels of anti-SRBC antibody and the specific cellular immune responses were measured by microhemagglutination test and footpad thickness, respectively. Moreover, splenocytes were checked for proliferation rate, respiratory burst, cytokine production and FoxP3+Treg cells frequency.

Results: Tretinoin markedly alleviated cellular immunity and concurrently potentiated humoral immunity after mice challenge with SRBCs. Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10. However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.

Conclusion: The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

No MeSH data available.


Related in: MedlinePlus

Evaluation of Tretinoin administration on FoxP3+Treg cells frequency. At the bleeding time, spleens were removed fromimmunized mice with SRBC. A single-cell suspension was prepared, and the cells were stained as detailed under materials and methods.Representative dot plots illustrated the regions and gates for immune phenotypic analysis. Lymphocytes were gated on a forward vs. sidescatter dot plot (A) and CD25+ T cells were gated by CD25/side scatter futures (B). Then, we analyzed CD4+ FoxP3+on CD25+ gated cells (C).(FL1, CD4+; FL2, CD25+; FL3, FoxP3+). Flow cytometry analysis demonstrated that. No significant change in the expression ofCD4+CD25+FoxP3+ was observed in the treatment group compared to the control mice (D). Data were shown as mean±SD
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4322144&req=5

Figure 4: Evaluation of Tretinoin administration on FoxP3+Treg cells frequency. At the bleeding time, spleens were removed fromimmunized mice with SRBC. A single-cell suspension was prepared, and the cells were stained as detailed under materials and methods.Representative dot plots illustrated the regions and gates for immune phenotypic analysis. Lymphocytes were gated on a forward vs. sidescatter dot plot (A) and CD25+ T cells were gated by CD25/side scatter futures (B). Then, we analyzed CD4+ FoxP3+on CD25+ gated cells (C).(FL1, CD4+; FL2, CD25+; FL3, FoxP3+). Flow cytometry analysis demonstrated that. No significant change in the expression ofCD4+CD25+FoxP3+ was observed in the treatment group compared to the control mice (D). Data were shown as mean±SD

Mentions: Evaluation method of Foxp3+Treg cells is shown in Figure 4A, B, and C. No significant change in the expression of these cells was observed in the treatment group compared to the control mice (Figure 4D).


New insight into the immunomodulatory mechanisms of Tretinoin in NMRI mice.

Froushani SM, Galeh HE - Iran J Basic Med Sci (2014)

Evaluation of Tretinoin administration on FoxP3+Treg cells frequency. At the bleeding time, spleens were removed fromimmunized mice with SRBC. A single-cell suspension was prepared, and the cells were stained as detailed under materials and methods.Representative dot plots illustrated the regions and gates for immune phenotypic analysis. Lymphocytes were gated on a forward vs. sidescatter dot plot (A) and CD25+ T cells were gated by CD25/side scatter futures (B). Then, we analyzed CD4+ FoxP3+on CD25+ gated cells (C).(FL1, CD4+; FL2, CD25+; FL3, FoxP3+). Flow cytometry analysis demonstrated that. No significant change in the expression ofCD4+CD25+FoxP3+ was observed in the treatment group compared to the control mice (D). Data were shown as mean±SD
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4322144&req=5

Figure 4: Evaluation of Tretinoin administration on FoxP3+Treg cells frequency. At the bleeding time, spleens were removed fromimmunized mice with SRBC. A single-cell suspension was prepared, and the cells were stained as detailed under materials and methods.Representative dot plots illustrated the regions and gates for immune phenotypic analysis. Lymphocytes were gated on a forward vs. sidescatter dot plot (A) and CD25+ T cells were gated by CD25/side scatter futures (B). Then, we analyzed CD4+ FoxP3+on CD25+ gated cells (C).(FL1, CD4+; FL2, CD25+; FL3, FoxP3+). Flow cytometry analysis demonstrated that. No significant change in the expression ofCD4+CD25+FoxP3+ was observed in the treatment group compared to the control mice (D). Data were shown as mean±SD
Mentions: Evaluation method of Foxp3+Treg cells is shown in Figure 4A, B, and C. No significant change in the expression of these cells was observed in the treatment group compared to the control mice (Figure 4D).

Bottom Line: Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10.However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: Recent evidence have proposed that Tretinoin produced in the gut preferentially promote differentiation of FoxP3+Treg cells but inhibits Th17 lymphocytes, and this may be the main immunomdulatory mechanism of Tretinoin in vivo. This study was done to investigate the effects of Tretinoin in outbred white mice after challenge with sheep red blood cells (SRBC).

Materials and methods: Twenty male NMRI-mice randomly allocated in two equal groups. Mice were treated with 1×10(9) SRBCs emulsified in CFA intraperitoneally twice with one weak interval. Animals were bled 5 days after last injection. Moreover, 48 hr before bleeding time, 1×10(9) SRBCs were injected into the left hind foot pad of mice. Tretinoin (25 mg/kg-every other day) were intraperitoneally injected into the treatment group from the beginning of the study and continued throughout the study. The levels of anti-SRBC antibody and the specific cellular immune responses were measured by microhemagglutination test and footpad thickness, respectively. Moreover, splenocytes were checked for proliferation rate, respiratory burst, cytokine production and FoxP3+Treg cells frequency.

Results: Tretinoin markedly alleviated cellular immunity and concurrently potentiated humoral immunity after mice challenge with SRBCs. Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10. However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.

Conclusion: The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

No MeSH data available.


Related in: MedlinePlus