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New insight into the immunomodulatory mechanisms of Tretinoin in NMRI mice.

Froushani SM, Galeh HE - Iran J Basic Med Sci (2014)

Bottom Line: Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10.However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: Recent evidence have proposed that Tretinoin produced in the gut preferentially promote differentiation of FoxP3+Treg cells but inhibits Th17 lymphocytes, and this may be the main immunomdulatory mechanism of Tretinoin in vivo. This study was done to investigate the effects of Tretinoin in outbred white mice after challenge with sheep red blood cells (SRBC).

Materials and methods: Twenty male NMRI-mice randomly allocated in two equal groups. Mice were treated with 1×10(9) SRBCs emulsified in CFA intraperitoneally twice with one weak interval. Animals were bled 5 days after last injection. Moreover, 48 hr before bleeding time, 1×10(9) SRBCs were injected into the left hind foot pad of mice. Tretinoin (25 mg/kg-every other day) were intraperitoneally injected into the treatment group from the beginning of the study and continued throughout the study. The levels of anti-SRBC antibody and the specific cellular immune responses were measured by microhemagglutination test and footpad thickness, respectively. Moreover, splenocytes were checked for proliferation rate, respiratory burst, cytokine production and FoxP3+Treg cells frequency.

Results: Tretinoin markedly alleviated cellular immunity and concurrently potentiated humoral immunity after mice challenge with SRBCs. Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10. However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.

Conclusion: The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

No MeSH data available.


Related in: MedlinePlus

Effects of Tretinoin administration on lymphocytes proliferation and respiratory burst in phagocytic cells. Splenocytes wereisolated from sensitized mice with SRBC. A) Splenocytes cultured with 50 µl PHA solution (1mg/ml) for 72 hr. Then, lymphocytesproliferation were evluated by MTT test. B) splenocytes with Staphylococcus aureus suspension and NBT were mixed and incubated for 30min as detailed under materials and methods. The reduced dye was extracted in dioxan and quantitated at 520 nm. The values werepresented as mean ± SD (*P<0.01, ** P<0.001 versus control mice)
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Figure 3: Effects of Tretinoin administration on lymphocytes proliferation and respiratory burst in phagocytic cells. Splenocytes wereisolated from sensitized mice with SRBC. A) Splenocytes cultured with 50 µl PHA solution (1mg/ml) for 72 hr. Then, lymphocytesproliferation were evluated by MTT test. B) splenocytes with Staphylococcus aureus suspension and NBT were mixed and incubated for 30min as detailed under materials and methods. The reduced dye was extracted in dioxan and quantitated at 520 nm. The values werepresented as mean ± SD (*P<0.01, ** P<0.001 versus control mice)

Mentions: A significant decrease in IL-17 and a significant increase in IL-10 secretion in cells from Tretinoin-treated mice were found compared to cells from vehicle-treated group (Figure 2). However, the level of IFN-γ was diminished in treatment group but this reduction was not significant (Figure 2). Moreover, splenocyte proliferation and respiratory burst showed a significant reduction in Tretinoin-treated mice compared to the normal control animals (Figure 3).


New insight into the immunomodulatory mechanisms of Tretinoin in NMRI mice.

Froushani SM, Galeh HE - Iran J Basic Med Sci (2014)

Effects of Tretinoin administration on lymphocytes proliferation and respiratory burst in phagocytic cells. Splenocytes wereisolated from sensitized mice with SRBC. A) Splenocytes cultured with 50 µl PHA solution (1mg/ml) for 72 hr. Then, lymphocytesproliferation were evluated by MTT test. B) splenocytes with Staphylococcus aureus suspension and NBT were mixed and incubated for 30min as detailed under materials and methods. The reduced dye was extracted in dioxan and quantitated at 520 nm. The values werepresented as mean ± SD (*P<0.01, ** P<0.001 versus control mice)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4322144&req=5

Figure 3: Effects of Tretinoin administration on lymphocytes proliferation and respiratory burst in phagocytic cells. Splenocytes wereisolated from sensitized mice with SRBC. A) Splenocytes cultured with 50 µl PHA solution (1mg/ml) for 72 hr. Then, lymphocytesproliferation were evluated by MTT test. B) splenocytes with Staphylococcus aureus suspension and NBT were mixed and incubated for 30min as detailed under materials and methods. The reduced dye was extracted in dioxan and quantitated at 520 nm. The values werepresented as mean ± SD (*P<0.01, ** P<0.001 versus control mice)
Mentions: A significant decrease in IL-17 and a significant increase in IL-10 secretion in cells from Tretinoin-treated mice were found compared to cells from vehicle-treated group (Figure 2). However, the level of IFN-γ was diminished in treatment group but this reduction was not significant (Figure 2). Moreover, splenocyte proliferation and respiratory burst showed a significant reduction in Tretinoin-treated mice compared to the normal control animals (Figure 3).

Bottom Line: Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10.However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: Recent evidence have proposed that Tretinoin produced in the gut preferentially promote differentiation of FoxP3+Treg cells but inhibits Th17 lymphocytes, and this may be the main immunomdulatory mechanism of Tretinoin in vivo. This study was done to investigate the effects of Tretinoin in outbred white mice after challenge with sheep red blood cells (SRBC).

Materials and methods: Twenty male NMRI-mice randomly allocated in two equal groups. Mice were treated with 1×10(9) SRBCs emulsified in CFA intraperitoneally twice with one weak interval. Animals were bled 5 days after last injection. Moreover, 48 hr before bleeding time, 1×10(9) SRBCs were injected into the left hind foot pad of mice. Tretinoin (25 mg/kg-every other day) were intraperitoneally injected into the treatment group from the beginning of the study and continued throughout the study. The levels of anti-SRBC antibody and the specific cellular immune responses were measured by microhemagglutination test and footpad thickness, respectively. Moreover, splenocytes were checked for proliferation rate, respiratory burst, cytokine production and FoxP3+Treg cells frequency.

Results: Tretinoin markedly alleviated cellular immunity and concurrently potentiated humoral immunity after mice challenge with SRBCs. Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10. However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.

Conclusion: The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

No MeSH data available.


Related in: MedlinePlus