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New insight into the immunomodulatory mechanisms of Tretinoin in NMRI mice.

Froushani SM, Galeh HE - Iran J Basic Med Sci (2014)

Bottom Line: Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10.However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: Recent evidence have proposed that Tretinoin produced in the gut preferentially promote differentiation of FoxP3+Treg cells but inhibits Th17 lymphocytes, and this may be the main immunomdulatory mechanism of Tretinoin in vivo. This study was done to investigate the effects of Tretinoin in outbred white mice after challenge with sheep red blood cells (SRBC).

Materials and methods: Twenty male NMRI-mice randomly allocated in two equal groups. Mice were treated with 1×10(9) SRBCs emulsified in CFA intraperitoneally twice with one weak interval. Animals were bled 5 days after last injection. Moreover, 48 hr before bleeding time, 1×10(9) SRBCs were injected into the left hind foot pad of mice. Tretinoin (25 mg/kg-every other day) were intraperitoneally injected into the treatment group from the beginning of the study and continued throughout the study. The levels of anti-SRBC antibody and the specific cellular immune responses were measured by microhemagglutination test and footpad thickness, respectively. Moreover, splenocytes were checked for proliferation rate, respiratory burst, cytokine production and FoxP3+Treg cells frequency.

Results: Tretinoin markedly alleviated cellular immunity and concurrently potentiated humoral immunity after mice challenge with SRBCs. Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10. However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.

Conclusion: The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

No MeSH data available.


Related in: MedlinePlus

Effects of administration of Tretinoin on cellularimmunity. Mice were intraperitoneally immunized twice with oneweak interval by 1×109 sheep red blood cells (SRBC) emulsified inCFA. 3 days after last intraperitoneal immunization, 1×109 SRBCsin 50 µl of PBS were administered subcutaneously into the lefthind foot pad of each mouse and the same volume of PBS wasinjected into the right foot pad as a negative control.Footpadthickness was measured before bleeding time with a dial caliperand the mean percentage increase in footpad thickness wasmeasured. The values were presented as mean ± S.D. (*P<0.001versus control mice)
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Figure 1: Effects of administration of Tretinoin on cellularimmunity. Mice were intraperitoneally immunized twice with oneweak interval by 1×109 sheep red blood cells (SRBC) emulsified inCFA. 3 days after last intraperitoneal immunization, 1×109 SRBCsin 50 µl of PBS were administered subcutaneously into the lefthind foot pad of each mouse and the same volume of PBS wasinjected into the right foot pad as a negative control.Footpadthickness was measured before bleeding time with a dial caliperand the mean percentage increase in footpad thickness wasmeasured. The values were presented as mean ± S.D. (*P<0.001versus control mice)

Mentions: Footpad thickness after challenge with SRBC was performed as an indicator for evaluation of delayed type of hypersensitivity (DTH) reaction. As shown in Figure 1, Tretinoin-treated mice showed significantly lower DTH responses than the control mice. Conversely, mean antibody titer in treatment group (260±11.22) was significantly higher than the mean antibody titer in control mice (35.87±9.76) (P<0.001).


New insight into the immunomodulatory mechanisms of Tretinoin in NMRI mice.

Froushani SM, Galeh HE - Iran J Basic Med Sci (2014)

Effects of administration of Tretinoin on cellularimmunity. Mice were intraperitoneally immunized twice with oneweak interval by 1×109 sheep red blood cells (SRBC) emulsified inCFA. 3 days after last intraperitoneal immunization, 1×109 SRBCsin 50 µl of PBS were administered subcutaneously into the lefthind foot pad of each mouse and the same volume of PBS wasinjected into the right foot pad as a negative control.Footpadthickness was measured before bleeding time with a dial caliperand the mean percentage increase in footpad thickness wasmeasured. The values were presented as mean ± S.D. (*P<0.001versus control mice)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4322144&req=5

Figure 1: Effects of administration of Tretinoin on cellularimmunity. Mice were intraperitoneally immunized twice with oneweak interval by 1×109 sheep red blood cells (SRBC) emulsified inCFA. 3 days after last intraperitoneal immunization, 1×109 SRBCsin 50 µl of PBS were administered subcutaneously into the lefthind foot pad of each mouse and the same volume of PBS wasinjected into the right foot pad as a negative control.Footpadthickness was measured before bleeding time with a dial caliperand the mean percentage increase in footpad thickness wasmeasured. The values were presented as mean ± S.D. (*P<0.001versus control mice)
Mentions: Footpad thickness after challenge with SRBC was performed as an indicator for evaluation of delayed type of hypersensitivity (DTH) reaction. As shown in Figure 1, Tretinoin-treated mice showed significantly lower DTH responses than the control mice. Conversely, mean antibody titer in treatment group (260±11.22) was significantly higher than the mean antibody titer in control mice (35.87±9.76) (P<0.001).

Bottom Line: Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10.However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: Recent evidence have proposed that Tretinoin produced in the gut preferentially promote differentiation of FoxP3+Treg cells but inhibits Th17 lymphocytes, and this may be the main immunomdulatory mechanism of Tretinoin in vivo. This study was done to investigate the effects of Tretinoin in outbred white mice after challenge with sheep red blood cells (SRBC).

Materials and methods: Twenty male NMRI-mice randomly allocated in two equal groups. Mice were treated with 1×10(9) SRBCs emulsified in CFA intraperitoneally twice with one weak interval. Animals were bled 5 days after last injection. Moreover, 48 hr before bleeding time, 1×10(9) SRBCs were injected into the left hind foot pad of mice. Tretinoin (25 mg/kg-every other day) were intraperitoneally injected into the treatment group from the beginning of the study and continued throughout the study. The levels of anti-SRBC antibody and the specific cellular immune responses were measured by microhemagglutination test and footpad thickness, respectively. Moreover, splenocytes were checked for proliferation rate, respiratory burst, cytokine production and FoxP3+Treg cells frequency.

Results: Tretinoin markedly alleviated cellular immunity and concurrently potentiated humoral immunity after mice challenge with SRBCs. Furthermore, aside from reducing NBT reduction and lymphocyte proliferation, Tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10. However, the level of IFN-γ and the frequency of FoxP3+Treg cells did not alter significantly.

Conclusion: The in vivo immunomudlatoty effects of Tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of FoxP3(+)Treg cells.

No MeSH data available.


Related in: MedlinePlus