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Effect of aerobic exercise training on non-alcoholic fatty liver disease induced by a high fat diet in C57BL/6 mice.

Cho J, Lee I, Kim D, Koh Y, Kong J, Lee S, Kang H - J Exerc Nutrition Biochem (2014)

Bottom Line: Outcomes included hepatic steatosis, insulin resistance, and expression of genes involved in mitochondrial function and/or fatty oxidation as well as de novo lipogenesis and/or triacylglycerol (TAG) synthesis.Treadmill running ameliorated impaired glucose tolerance and insulin resistance secondary to the HFD.The beneficial effects of treadmill running were associated with enhanced molecular markers of mitochondrial function and/or fatty acids oxidation (i.e., PPARα and CPT1a mRNAs, pAMPK/AMPK, pACC, and SIRT1 protein) as well as suppressed expression of de novo lipogenesis and/or TAG synthesis (i.e., SREBP1c, lipin1 and FAS mRNAs) in the liver.

View Article: PubMed Central - PubMed

Affiliation: College of Sport Science, Sungkyunkwan University, Suwon, Korea.

ABSTRACT

Purpose: The aim of this study was to investigate the effects of aerobic exercise training on a high fat diet (HFD)-induced fatty liver and its metabolic complications in C57BL/6 mice.

Methods: Mice at 5-month old (n = 30) were randomly assigned to standard chow (SC + CON, n = 10) and high-fat diet (HFD, n = 20), and they were subjected to SC and HFD, respectively, for 23-week. After 15-week of HFD, mice in the HFD group were further assigned to HFD (HFD + CON, n = 10) or exercise training (HFD + EX, n = 10) groups. The HFD + EX mice were subjected to aerobic treadmill running during the last 8-week of the 23-week HFD course. Outcomes included hepatic steatosis, insulin resistance, and expression of genes involved in mitochondrial function and/or fatty oxidation as well as de novo lipogenesis and/or triacylglycerol (TAG) synthesis.

Results: Treadmill running ameliorated impaired glucose tolerance and insulin resistance secondary to the HFD. The beneficial effects of treadmill running were associated with enhanced molecular markers of mitochondrial function and/or fatty acids oxidation (i.e., PPARα and CPT1a mRNAs, pAMPK/AMPK, pACC, and SIRT1 protein) as well as suppressed expression of de novo lipogenesis and/or TAG synthesis (i.e., SREBP1c, lipin1 and FAS mRNAs) in the liver.

Conclusion: The current findings suggest that aerobic exercise training is an effective and non-pharmacological means to combat fatty liver and its metabolic complications in HFD-induced obese mice.

No MeSH data available.


Related in: MedlinePlus

Effects of aerobic exercise training on metabolic parameters (A) Glucose homeostasis was assessed by an intraperitoneal glucose tolerance test (IPGTT) and (B) intraperitoneal insulin tolerance test (IPITT). (C) The area under the curves for GTT and (D) ITT were calculated. Data are presented as means ± SD. Superscripts with different letters (i.e., a-b, b-c) indicate significant group differences. LSD post hoc tests were used for multiple comparisons.
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f1-jenb-18-4-339: Effects of aerobic exercise training on metabolic parameters (A) Glucose homeostasis was assessed by an intraperitoneal glucose tolerance test (IPGTT) and (B) intraperitoneal insulin tolerance test (IPITT). (C) The area under the curves for GTT and (D) ITT were calculated. Data are presented as means ± SD. Superscripts with different letters (i.e., a-b, b-c) indicate significant group differences. LSD post hoc tests were used for multiple comparisons.

Mentions: Fig. 1 represents the outcomes of insulin resistance parameters. The HFD + CON (p < 0.001) and HFD + EX (p < 0.001) mice had significantly higher values in areas under the curve (AUC) for the glucose tolerance test (GTT) and the insulin tolerance test (ITT) than the SC mice. The HFD + EX mice had significantly (p < 0.05) lower values of AUC for the GTT and ITT versus HFD + CON mice. Similarly, the HFD + CON and HFD + EX mice had significantly higher values of fasting glucose (p < 0.001) and insulin (p < 0.001) levels compared with SC + CON (Table 2). The HFD + EX mice had significantly lower values of fasting glucose (p < 0.05) and insulin (p < 0.05) concentration versus HFD + CON mice (Table 2).


Effect of aerobic exercise training on non-alcoholic fatty liver disease induced by a high fat diet in C57BL/6 mice.

Cho J, Lee I, Kim D, Koh Y, Kong J, Lee S, Kang H - J Exerc Nutrition Biochem (2014)

Effects of aerobic exercise training on metabolic parameters (A) Glucose homeostasis was assessed by an intraperitoneal glucose tolerance test (IPGTT) and (B) intraperitoneal insulin tolerance test (IPITT). (C) The area under the curves for GTT and (D) ITT were calculated. Data are presented as means ± SD. Superscripts with different letters (i.e., a-b, b-c) indicate significant group differences. LSD post hoc tests were used for multiple comparisons.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4322024&req=5

f1-jenb-18-4-339: Effects of aerobic exercise training on metabolic parameters (A) Glucose homeostasis was assessed by an intraperitoneal glucose tolerance test (IPGTT) and (B) intraperitoneal insulin tolerance test (IPITT). (C) The area under the curves for GTT and (D) ITT were calculated. Data are presented as means ± SD. Superscripts with different letters (i.e., a-b, b-c) indicate significant group differences. LSD post hoc tests were used for multiple comparisons.
Mentions: Fig. 1 represents the outcomes of insulin resistance parameters. The HFD + CON (p < 0.001) and HFD + EX (p < 0.001) mice had significantly higher values in areas under the curve (AUC) for the glucose tolerance test (GTT) and the insulin tolerance test (ITT) than the SC mice. The HFD + EX mice had significantly (p < 0.05) lower values of AUC for the GTT and ITT versus HFD + CON mice. Similarly, the HFD + CON and HFD + EX mice had significantly higher values of fasting glucose (p < 0.001) and insulin (p < 0.001) levels compared with SC + CON (Table 2). The HFD + EX mice had significantly lower values of fasting glucose (p < 0.05) and insulin (p < 0.05) concentration versus HFD + CON mice (Table 2).

Bottom Line: Outcomes included hepatic steatosis, insulin resistance, and expression of genes involved in mitochondrial function and/or fatty oxidation as well as de novo lipogenesis and/or triacylglycerol (TAG) synthesis.Treadmill running ameliorated impaired glucose tolerance and insulin resistance secondary to the HFD.The beneficial effects of treadmill running were associated with enhanced molecular markers of mitochondrial function and/or fatty acids oxidation (i.e., PPARα and CPT1a mRNAs, pAMPK/AMPK, pACC, and SIRT1 protein) as well as suppressed expression of de novo lipogenesis and/or TAG synthesis (i.e., SREBP1c, lipin1 and FAS mRNAs) in the liver.

View Article: PubMed Central - PubMed

Affiliation: College of Sport Science, Sungkyunkwan University, Suwon, Korea.

ABSTRACT

Purpose: The aim of this study was to investigate the effects of aerobic exercise training on a high fat diet (HFD)-induced fatty liver and its metabolic complications in C57BL/6 mice.

Methods: Mice at 5-month old (n = 30) were randomly assigned to standard chow (SC + CON, n = 10) and high-fat diet (HFD, n = 20), and they were subjected to SC and HFD, respectively, for 23-week. After 15-week of HFD, mice in the HFD group were further assigned to HFD (HFD + CON, n = 10) or exercise training (HFD + EX, n = 10) groups. The HFD + EX mice were subjected to aerobic treadmill running during the last 8-week of the 23-week HFD course. Outcomes included hepatic steatosis, insulin resistance, and expression of genes involved in mitochondrial function and/or fatty oxidation as well as de novo lipogenesis and/or triacylglycerol (TAG) synthesis.

Results: Treadmill running ameliorated impaired glucose tolerance and insulin resistance secondary to the HFD. The beneficial effects of treadmill running were associated with enhanced molecular markers of mitochondrial function and/or fatty acids oxidation (i.e., PPARα and CPT1a mRNAs, pAMPK/AMPK, pACC, and SIRT1 protein) as well as suppressed expression of de novo lipogenesis and/or TAG synthesis (i.e., SREBP1c, lipin1 and FAS mRNAs) in the liver.

Conclusion: The current findings suggest that aerobic exercise training is an effective and non-pharmacological means to combat fatty liver and its metabolic complications in HFD-induced obese mice.

No MeSH data available.


Related in: MedlinePlus