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Regularities of Anthocyanins Retention in RP HPLC for "Water-Acetonitrile-Phosphoric Acid" Mobile Phases.

Deineka VI, Deineka LA, Saenko II - J Anal Methods Chem (2015)

Bottom Line: The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis.The differences and similarities of anthocyanins separation were revealed.It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology and Chemistry, Federal State Autonomous Educational Institution of Higher Professional Education "Belgorod National Research University", Pobeda Street 85, Belgorod 308015, Russia.

ABSTRACT
The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis. The differences and similarities of anthocyanins separation were revealed. It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

No MeSH data available.


Related in: MedlinePlus

Comparison of separation maps for Cy3AGlu (1); Cy3XRut (2); and Cy3Rut (3) in System 1 (empty markers with lines) and in System 2 (corresponding black markers without lines).
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fig7: Comparison of separation maps for Cy3AGlu (1); Cy3XRut (2); and Cy3Rut (3) in System 1 (empty markers with lines) and in System 2 (corresponding black markers without lines).

Mentions: Finely, the exchange of HCOOH by phosphoric acid also leads to slight decrease of relative retention of di- and trisaccharides, Figure 7, but no significant alterations of solutes separation selectivity were found.


Regularities of Anthocyanins Retention in RP HPLC for "Water-Acetonitrile-Phosphoric Acid" Mobile Phases.

Deineka VI, Deineka LA, Saenko II - J Anal Methods Chem (2015)

Comparison of separation maps for Cy3AGlu (1); Cy3XRut (2); and Cy3Rut (3) in System 1 (empty markers with lines) and in System 2 (corresponding black markers without lines).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4321844&req=5

fig7: Comparison of separation maps for Cy3AGlu (1); Cy3XRut (2); and Cy3Rut (3) in System 1 (empty markers with lines) and in System 2 (corresponding black markers without lines).
Mentions: Finely, the exchange of HCOOH by phosphoric acid also leads to slight decrease of relative retention of di- and trisaccharides, Figure 7, but no significant alterations of solutes separation selectivity were found.

Bottom Line: The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis.The differences and similarities of anthocyanins separation were revealed.It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology and Chemistry, Federal State Autonomous Educational Institution of Higher Professional Education "Belgorod National Research University", Pobeda Street 85, Belgorod 308015, Russia.

ABSTRACT
The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis. The differences and similarities of anthocyanins separation were revealed. It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

No MeSH data available.


Related in: MedlinePlus