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Regularities of Anthocyanins Retention in RP HPLC for "Water-Acetonitrile-Phosphoric Acid" Mobile Phases.

Deineka VI, Deineka LA, Saenko II - J Anal Methods Chem (2015)

Bottom Line: The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis.The differences and similarities of anthocyanins separation were revealed.It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology and Chemistry, Federal State Autonomous Educational Institution of Higher Professional Education "Belgorod National Research University", Pobeda Street 85, Belgorod 308015, Russia.

ABSTRACT
The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis. The differences and similarities of anthocyanins separation were revealed. It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

No MeSH data available.


Related in: MedlinePlus

Dependence of Cy3Glu retention upon acetonitrile volume fraction (ϕ, %) in System 1 (A) and System 2 (B). Chromatographic column: 4.6 × 250 mm Symmetry C18.
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fig2: Dependence of Cy3Glu retention upon acetonitrile volume fraction (ϕ, %) in System 1 (A) and System 2 (B). Chromatographic column: 4.6 × 250 mm Symmetry C18.

Mentions: The dependence of retention of sample anthocyanin cyanidin-3-glucoside, Cy3Glu (Figure 2), upon volume fraction of CH3CN in a mobile phase is rather linear function according to Snyder equation [20] (1) for System 1 (R2 = 0.9998) as well as for System 2 (R2 = 0.998):(1)log⁡kϕi=log⁡kwi−Si·ϕ,where kϕ(i) is retention factor of Cy3Glu at a given ϕ, volume fraction of CH3CN in a mobile phase, and log⁡kw(i) and S(i) are linear approximation constants.


Regularities of Anthocyanins Retention in RP HPLC for "Water-Acetonitrile-Phosphoric Acid" Mobile Phases.

Deineka VI, Deineka LA, Saenko II - J Anal Methods Chem (2015)

Dependence of Cy3Glu retention upon acetonitrile volume fraction (ϕ, %) in System 1 (A) and System 2 (B). Chromatographic column: 4.6 × 250 mm Symmetry C18.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4321844&req=5

fig2: Dependence of Cy3Glu retention upon acetonitrile volume fraction (ϕ, %) in System 1 (A) and System 2 (B). Chromatographic column: 4.6 × 250 mm Symmetry C18.
Mentions: The dependence of retention of sample anthocyanin cyanidin-3-glucoside, Cy3Glu (Figure 2), upon volume fraction of CH3CN in a mobile phase is rather linear function according to Snyder equation [20] (1) for System 1 (R2 = 0.9998) as well as for System 2 (R2 = 0.998):(1)log⁡kϕi=log⁡kwi−Si·ϕ,where kϕ(i) is retention factor of Cy3Glu at a given ϕ, volume fraction of CH3CN in a mobile phase, and log⁡kw(i) and S(i) are linear approximation constants.

Bottom Line: The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis.The differences and similarities of anthocyanins separation were revealed.It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology and Chemistry, Federal State Autonomous Educational Institution of Higher Professional Education "Belgorod National Research University", Pobeda Street 85, Belgorod 308015, Russia.

ABSTRACT
The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis. The differences and similarities of anthocyanins separation were revealed. It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

No MeSH data available.


Related in: MedlinePlus