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Regularities of Anthocyanins Retention in RP HPLC for "Water-Acetonitrile-Phosphoric Acid" Mobile Phases.

Deineka VI, Deineka LA, Saenko II - J Anal Methods Chem (2015)

Bottom Line: The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis.The differences and similarities of anthocyanins separation were revealed.It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology and Chemistry, Federal State Autonomous Educational Institution of Higher Professional Education "Belgorod National Research University", Pobeda Street 85, Belgorod 308015, Russia.

ABSTRACT
The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis. The differences and similarities of anthocyanins separation were revealed. It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

No MeSH data available.


Related in: MedlinePlus

Flavylium form of 3-glycosides of six common anthocyanidins. R3′ and R5′ are H and H for pelargonidin (Pg); OH and H for cyanidin (Cy); OH and OH for delphinidin (Dp); OCH3 and H for peonidin (Pn); OCH3 and OH for petunidin (Pt); OCH3 and OCH3 for malvidin (Mv).
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fig1: Flavylium form of 3-glycosides of six common anthocyanidins. R3′ and R5′ are H and H for pelargonidin (Pg); OH and H for cyanidin (Cy); OH and OH for delphinidin (Dp); OCH3 and H for peonidin (Pn); OCH3 and OH for petunidin (Pt); OCH3 and OCH3 for malvidin (Mv).

Mentions: Anthocyanins are powerful water-soluble antioxidants of flavonoids class with health promoting effect [1, 2]. The coloured flavylium form of anthocyanins is a reason to regard them as natural food colorants [2]. The latter explains a high scientific and technological interest to the substances. Anthocyanins are synthesized in plant tissue, mainly in fruits, flowers and for some species in leaves as a rule as a complex mixture of compounds with different structures [3]. Anthocyanins are glycosides of anthocyanidins (Figure 1), with great varieties of more than 600 anthocyanin structures found in plant sources [4] though only six structures of the latter cover the majority of the structures due to glycosylation type variability [5].


Regularities of Anthocyanins Retention in RP HPLC for "Water-Acetonitrile-Phosphoric Acid" Mobile Phases.

Deineka VI, Deineka LA, Saenko II - J Anal Methods Chem (2015)

Flavylium form of 3-glycosides of six common anthocyanidins. R3′ and R5′ are H and H for pelargonidin (Pg); OH and H for cyanidin (Cy); OH and OH for delphinidin (Dp); OCH3 and H for peonidin (Pn); OCH3 and OH for petunidin (Pt); OCH3 and OCH3 for malvidin (Mv).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4321844&req=5

fig1: Flavylium form of 3-glycosides of six common anthocyanidins. R3′ and R5′ are H and H for pelargonidin (Pg); OH and H for cyanidin (Cy); OH and OH for delphinidin (Dp); OCH3 and H for peonidin (Pn); OCH3 and OH for petunidin (Pt); OCH3 and OCH3 for malvidin (Mv).
Mentions: Anthocyanins are powerful water-soluble antioxidants of flavonoids class with health promoting effect [1, 2]. The coloured flavylium form of anthocyanins is a reason to regard them as natural food colorants [2]. The latter explains a high scientific and technological interest to the substances. Anthocyanins are synthesized in plant tissue, mainly in fruits, flowers and for some species in leaves as a rule as a complex mixture of compounds with different structures [3]. Anthocyanins are glycosides of anthocyanidins (Figure 1), with great varieties of more than 600 anthocyanin structures found in plant sources [4] though only six structures of the latter cover the majority of the structures due to glycosylation type variability [5].

Bottom Line: The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis.The differences and similarities of anthocyanins separation were revealed.It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology and Chemistry, Federal State Autonomous Educational Institution of Higher Professional Education "Belgorod National Research University", Pobeda Street 85, Belgorod 308015, Russia.

ABSTRACT
The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the "acetonitrile-water" mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis. The differences and similarities of anthocyanins separation were revealed. It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

No MeSH data available.


Related in: MedlinePlus