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Characterization of foot-and-mouth disease viruses (FMDVs) from Ugandan cattle outbreaks during 2012-2013: evidence for circulation of multiple serotypes.

Namatovu A, Tjørnehøj K, Belsham GJ, Dhikusooka MT, Wekesa SN, Muwanika VB, Siegismund HR, Ayebazibwe C - PLoS ONE (2015)

Bottom Line: Overall, 61/79 (77%) of the cattle sera were positive for antibodies against FMDV by PrioCHECK FMDV NS ELISA and solid phase blocking ELISA detected titres ≥ 80 for serotypes O, SAT 1, SAT 2 and SAT 3 in 41, 45, 30 and 45 of these 61 seropositive samples, respectively.This study shows that within a period of less than one year, FMD outbreaks in Uganda were caused by four different serotypes namely O, A, SAT 1 and SAT 2.The value of incorporating serotype A antigen into the imported vaccines along with the current serotype O, SAT 1 and SAT 2 strains should be considered.

View Article: PubMed Central - PubMed

Affiliation: National Animal Disease Diagnostics and Epidemiology Centre, Ministry of Agriculture Animal Industry and Fisheries, P. O. Box 513, Entebbe, Uganda; Department of Biotechnical and Diagnostic Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda.

ABSTRACT
To investigate the foot-and-mouth disease virus (FMDV) serotypes circulating in Uganda's cattle population, both serological and virological analyses of samples from outbreaks that occurred during 2012-2013 were performed. Altogether, 79 sera and 60 oropharyngeal fluid (OP)/tissue/oral swab samples were collected from herds with reported FMD outbreaks in seven different Ugandan districts. Overall, 61/79 (77%) of the cattle sera were positive for antibodies against FMDV by PrioCHECK FMDV NS ELISA and solid phase blocking ELISA detected titres ≥ 80 for serotypes O, SAT 1, SAT 2 and SAT 3 in 41, 45, 30 and 45 of these 61 seropositive samples, respectively. Virus neutralisation tests detected the highest levels of neutralising antibodies (titres ≥ 45) against serotype O in the herds from Kween and Rakai districts, against SAT 1 in the herd from Nwoya district and against SAT 2 in the herds from Kiruhura, Isingiro and Ntungamo districts. The isolation of a SAT 2 FMDV from Isingiro was consistent with the detection of high levels of neutralising antibodies against SAT 2; sequencing (for the VP1 coding region) indicated that this virus belonged to lineage I within this serotype, like the currently used vaccine strain. From the Wakiso district 11 tissue/swab samples were collected; serotype A FMDV, genotype Africa (G-I), was isolated from the epithelial samples. This study shows that within a period of less than one year, FMD outbreaks in Uganda were caused by four different serotypes namely O, A, SAT 1 and SAT 2. Therefore, to enhance the control of FMD in Uganda, there is need for efficient and timely determination of outbreak virus strains/serotypes and vaccine matching. The value of incorporating serotype A antigen into the imported vaccines along with the current serotype O, SAT 1 and SAT 2 strains should be considered.

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Alignment of serotype SAT 2 FMDV VP1 amino acid sequences.The VP1 amino acid sequences were inferred from one of the 2013 outbreak nucleotide sequences (marked with an asterisk (*)) and earlier SAT 2 FMDV strains. Identities with the reference vaccine strain sequence (K52/84**) are indicated by dots. Similar amino acid variation exists between the reference sequence and the Ugandan 2013 isolates and a Kenyan outbreak isolate (K125/12*) sequence (unpublished data).
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pone.0114811.g005: Alignment of serotype SAT 2 FMDV VP1 amino acid sequences.The VP1 amino acid sequences were inferred from one of the 2013 outbreak nucleotide sequences (marked with an asterisk (*)) and earlier SAT 2 FMDV strains. Identities with the reference vaccine strain sequence (K52/84**) are indicated by dots. Similar amino acid variation exists between the reference sequence and the Ugandan 2013 isolates and a Kenyan outbreak isolate (K125/12*) sequence (unpublished data).

Mentions: Eighty five (13%) variable sites were determined across the 642 nt between the Isingiro 2013 SAT 2 outbreak sequences and the vaccine strain (K52/84) which encode substitutions of 12/214 (6%) amino acids in the deduced protein sequence (Fig. 5). The RGD motif (residues 144–146) within these viruses was conserved and so was the flanking region of the RGD motif as far as the -5 and +10 positions (Fig. 5). Conservative changes occurred at residue 138 where Asp (D) was replaced by Glu (E) and at position 157 where Ser (S) was replaced by Thr (T), both residues are located in the flanking region of the RGD motif cell attachment site [44, 45] which also constitutes an important antigenic site.


Characterization of foot-and-mouth disease viruses (FMDVs) from Ugandan cattle outbreaks during 2012-2013: evidence for circulation of multiple serotypes.

Namatovu A, Tjørnehøj K, Belsham GJ, Dhikusooka MT, Wekesa SN, Muwanika VB, Siegismund HR, Ayebazibwe C - PLoS ONE (2015)

Alignment of serotype SAT 2 FMDV VP1 amino acid sequences.The VP1 amino acid sequences were inferred from one of the 2013 outbreak nucleotide sequences (marked with an asterisk (*)) and earlier SAT 2 FMDV strains. Identities with the reference vaccine strain sequence (K52/84**) are indicated by dots. Similar amino acid variation exists between the reference sequence and the Ugandan 2013 isolates and a Kenyan outbreak isolate (K125/12*) sequence (unpublished data).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321839&req=5

pone.0114811.g005: Alignment of serotype SAT 2 FMDV VP1 amino acid sequences.The VP1 amino acid sequences were inferred from one of the 2013 outbreak nucleotide sequences (marked with an asterisk (*)) and earlier SAT 2 FMDV strains. Identities with the reference vaccine strain sequence (K52/84**) are indicated by dots. Similar amino acid variation exists between the reference sequence and the Ugandan 2013 isolates and a Kenyan outbreak isolate (K125/12*) sequence (unpublished data).
Mentions: Eighty five (13%) variable sites were determined across the 642 nt between the Isingiro 2013 SAT 2 outbreak sequences and the vaccine strain (K52/84) which encode substitutions of 12/214 (6%) amino acids in the deduced protein sequence (Fig. 5). The RGD motif (residues 144–146) within these viruses was conserved and so was the flanking region of the RGD motif as far as the -5 and +10 positions (Fig. 5). Conservative changes occurred at residue 138 where Asp (D) was replaced by Glu (E) and at position 157 where Ser (S) was replaced by Thr (T), both residues are located in the flanking region of the RGD motif cell attachment site [44, 45] which also constitutes an important antigenic site.

Bottom Line: Overall, 61/79 (77%) of the cattle sera were positive for antibodies against FMDV by PrioCHECK FMDV NS ELISA and solid phase blocking ELISA detected titres ≥ 80 for serotypes O, SAT 1, SAT 2 and SAT 3 in 41, 45, 30 and 45 of these 61 seropositive samples, respectively.This study shows that within a period of less than one year, FMD outbreaks in Uganda were caused by four different serotypes namely O, A, SAT 1 and SAT 2.The value of incorporating serotype A antigen into the imported vaccines along with the current serotype O, SAT 1 and SAT 2 strains should be considered.

View Article: PubMed Central - PubMed

Affiliation: National Animal Disease Diagnostics and Epidemiology Centre, Ministry of Agriculture Animal Industry and Fisheries, P. O. Box 513, Entebbe, Uganda; Department of Biotechnical and Diagnostic Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda.

ABSTRACT
To investigate the foot-and-mouth disease virus (FMDV) serotypes circulating in Uganda's cattle population, both serological and virological analyses of samples from outbreaks that occurred during 2012-2013 were performed. Altogether, 79 sera and 60 oropharyngeal fluid (OP)/tissue/oral swab samples were collected from herds with reported FMD outbreaks in seven different Ugandan districts. Overall, 61/79 (77%) of the cattle sera were positive for antibodies against FMDV by PrioCHECK FMDV NS ELISA and solid phase blocking ELISA detected titres ≥ 80 for serotypes O, SAT 1, SAT 2 and SAT 3 in 41, 45, 30 and 45 of these 61 seropositive samples, respectively. Virus neutralisation tests detected the highest levels of neutralising antibodies (titres ≥ 45) against serotype O in the herds from Kween and Rakai districts, against SAT 1 in the herd from Nwoya district and against SAT 2 in the herds from Kiruhura, Isingiro and Ntungamo districts. The isolation of a SAT 2 FMDV from Isingiro was consistent with the detection of high levels of neutralising antibodies against SAT 2; sequencing (for the VP1 coding region) indicated that this virus belonged to lineage I within this serotype, like the currently used vaccine strain. From the Wakiso district 11 tissue/swab samples were collected; serotype A FMDV, genotype Africa (G-I), was isolated from the epithelial samples. This study shows that within a period of less than one year, FMD outbreaks in Uganda were caused by four different serotypes namely O, A, SAT 1 and SAT 2. Therefore, to enhance the control of FMD in Uganda, there is need for efficient and timely determination of outbreak virus strains/serotypes and vaccine matching. The value of incorporating serotype A antigen into the imported vaccines along with the current serotype O, SAT 1 and SAT 2 strains should be considered.

Show MeSH
Related in: MedlinePlus