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Characterization of foot-and-mouth disease viruses (FMDVs) from Ugandan cattle outbreaks during 2012-2013: evidence for circulation of multiple serotypes.

Namatovu A, Tjørnehøj K, Belsham GJ, Dhikusooka MT, Wekesa SN, Muwanika VB, Siegismund HR, Ayebazibwe C - PLoS ONE (2015)

Bottom Line: Overall, 61/79 (77%) of the cattle sera were positive for antibodies against FMDV by PrioCHECK FMDV NS ELISA and solid phase blocking ELISA detected titres ≥ 80 for serotypes O, SAT 1, SAT 2 and SAT 3 in 41, 45, 30 and 45 of these 61 seropositive samples, respectively.This study shows that within a period of less than one year, FMD outbreaks in Uganda were caused by four different serotypes namely O, A, SAT 1 and SAT 2.The value of incorporating serotype A antigen into the imported vaccines along with the current serotype O, SAT 1 and SAT 2 strains should be considered.

View Article: PubMed Central - PubMed

Affiliation: National Animal Disease Diagnostics and Epidemiology Centre, Ministry of Agriculture Animal Industry and Fisheries, P. O. Box 513, Entebbe, Uganda; Department of Biotechnical and Diagnostic Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda.

ABSTRACT
To investigate the foot-and-mouth disease virus (FMDV) serotypes circulating in Uganda's cattle population, both serological and virological analyses of samples from outbreaks that occurred during 2012-2013 were performed. Altogether, 79 sera and 60 oropharyngeal fluid (OP)/tissue/oral swab samples were collected from herds with reported FMD outbreaks in seven different Ugandan districts. Overall, 61/79 (77%) of the cattle sera were positive for antibodies against FMDV by PrioCHECK FMDV NS ELISA and solid phase blocking ELISA detected titres ≥ 80 for serotypes O, SAT 1, SAT 2 and SAT 3 in 41, 45, 30 and 45 of these 61 seropositive samples, respectively. Virus neutralisation tests detected the highest levels of neutralising antibodies (titres ≥ 45) against serotype O in the herds from Kween and Rakai districts, against SAT 1 in the herd from Nwoya district and against SAT 2 in the herds from Kiruhura, Isingiro and Ntungamo districts. The isolation of a SAT 2 FMDV from Isingiro was consistent with the detection of high levels of neutralising antibodies against SAT 2; sequencing (for the VP1 coding region) indicated that this virus belonged to lineage I within this serotype, like the currently used vaccine strain. From the Wakiso district 11 tissue/swab samples were collected; serotype A FMDV, genotype Africa (G-I), was isolated from the epithelial samples. This study shows that within a period of less than one year, FMD outbreaks in Uganda were caused by four different serotypes namely O, A, SAT 1 and SAT 2. Therefore, to enhance the control of FMD in Uganda, there is need for efficient and timely determination of outbreak virus strains/serotypes and vaccine matching. The value of incorporating serotype A antigen into the imported vaccines along with the current serotype O, SAT 1 and SAT 2 strains should be considered.

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The location of reported FMD outbreaks within Uganda in 2012–2013.The map shows the seven districts of Uganda that reported FMD outbreaks during 2012–2013. These districts were: Kiruhura, Kween and Nwoya during the 2012 outbreaks plus Isingiro, Ntungamo, Rakai and Wakiso during the 2013 outbreaks.
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pone.0114811.g001: The location of reported FMD outbreaks within Uganda in 2012–2013.The map shows the seven districts of Uganda that reported FMD outbreaks during 2012–2013. These districts were: Kiruhura, Kween and Nwoya during the 2012 outbreaks plus Isingiro, Ntungamo, Rakai and Wakiso during the 2013 outbreaks.

Mentions: This study was performed using outbreak samples collected from Ugandan districts with reported FMD outbreaks in 2012–2013. In 2012, 33 cattle sera were submitted to the National Animal Disease Diagnostic and Epidemiological Centre (NADDEC) from three districts (Kiruhura, Kween and Nwoya) (Fig. 1). In 2013, oral epithelial tissues, oral swabs and/or oropharyngeal fluids (OPs) were collected from one outbreak herd in each of Isingiro, Ntungamo, Rakai and Wakiso districts and in addition sera were collected from these herds in Isingiro, Ntungamo and Rakai, totaling 46 sera, 30 OPs, 16 epithelial tissues and 14 oral swabs. These samples were collected following requests by the respective District Veterinary Officers (DVOs) and subsequent approval by the Commissioner Animal Health, Ministry of Agriculture Animal Industry and Fisheries. The DVOs sought the consent of each herd owner before sampling, and samples were collected from, Isingiro District (GPS: 36M 0248868, UTM 9918082), Ntungamo District (GPS: 36M 019262, UTM 989192), Rakai District (GPS: 36M 0315178, UTM 9894397) and Wakiso District (GPS: 36N 0447204, UTM 0041487). No GPS coordinates are available for the samples submitted in 2012.The farmers were interviewed about the suspected source of the outbreak, previous outbreaks, last vaccination and type of vaccine used, while the DVOs were interviewed about the history of outbreaks and vaccination, the vaccine used and the instituted control measures.


Characterization of foot-and-mouth disease viruses (FMDVs) from Ugandan cattle outbreaks during 2012-2013: evidence for circulation of multiple serotypes.

Namatovu A, Tjørnehøj K, Belsham GJ, Dhikusooka MT, Wekesa SN, Muwanika VB, Siegismund HR, Ayebazibwe C - PLoS ONE (2015)

The location of reported FMD outbreaks within Uganda in 2012–2013.The map shows the seven districts of Uganda that reported FMD outbreaks during 2012–2013. These districts were: Kiruhura, Kween and Nwoya during the 2012 outbreaks plus Isingiro, Ntungamo, Rakai and Wakiso during the 2013 outbreaks.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321839&req=5

pone.0114811.g001: The location of reported FMD outbreaks within Uganda in 2012–2013.The map shows the seven districts of Uganda that reported FMD outbreaks during 2012–2013. These districts were: Kiruhura, Kween and Nwoya during the 2012 outbreaks plus Isingiro, Ntungamo, Rakai and Wakiso during the 2013 outbreaks.
Mentions: This study was performed using outbreak samples collected from Ugandan districts with reported FMD outbreaks in 2012–2013. In 2012, 33 cattle sera were submitted to the National Animal Disease Diagnostic and Epidemiological Centre (NADDEC) from three districts (Kiruhura, Kween and Nwoya) (Fig. 1). In 2013, oral epithelial tissues, oral swabs and/or oropharyngeal fluids (OPs) were collected from one outbreak herd in each of Isingiro, Ntungamo, Rakai and Wakiso districts and in addition sera were collected from these herds in Isingiro, Ntungamo and Rakai, totaling 46 sera, 30 OPs, 16 epithelial tissues and 14 oral swabs. These samples were collected following requests by the respective District Veterinary Officers (DVOs) and subsequent approval by the Commissioner Animal Health, Ministry of Agriculture Animal Industry and Fisheries. The DVOs sought the consent of each herd owner before sampling, and samples were collected from, Isingiro District (GPS: 36M 0248868, UTM 9918082), Ntungamo District (GPS: 36M 019262, UTM 989192), Rakai District (GPS: 36M 0315178, UTM 9894397) and Wakiso District (GPS: 36N 0447204, UTM 0041487). No GPS coordinates are available for the samples submitted in 2012.The farmers were interviewed about the suspected source of the outbreak, previous outbreaks, last vaccination and type of vaccine used, while the DVOs were interviewed about the history of outbreaks and vaccination, the vaccine used and the instituted control measures.

Bottom Line: Overall, 61/79 (77%) of the cattle sera were positive for antibodies against FMDV by PrioCHECK FMDV NS ELISA and solid phase blocking ELISA detected titres ≥ 80 for serotypes O, SAT 1, SAT 2 and SAT 3 in 41, 45, 30 and 45 of these 61 seropositive samples, respectively.This study shows that within a period of less than one year, FMD outbreaks in Uganda were caused by four different serotypes namely O, A, SAT 1 and SAT 2.The value of incorporating serotype A antigen into the imported vaccines along with the current serotype O, SAT 1 and SAT 2 strains should be considered.

View Article: PubMed Central - PubMed

Affiliation: National Animal Disease Diagnostics and Epidemiology Centre, Ministry of Agriculture Animal Industry and Fisheries, P. O. Box 513, Entebbe, Uganda; Department of Biotechnical and Diagnostic Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda.

ABSTRACT
To investigate the foot-and-mouth disease virus (FMDV) serotypes circulating in Uganda's cattle population, both serological and virological analyses of samples from outbreaks that occurred during 2012-2013 were performed. Altogether, 79 sera and 60 oropharyngeal fluid (OP)/tissue/oral swab samples were collected from herds with reported FMD outbreaks in seven different Ugandan districts. Overall, 61/79 (77%) of the cattle sera were positive for antibodies against FMDV by PrioCHECK FMDV NS ELISA and solid phase blocking ELISA detected titres ≥ 80 for serotypes O, SAT 1, SAT 2 and SAT 3 in 41, 45, 30 and 45 of these 61 seropositive samples, respectively. Virus neutralisation tests detected the highest levels of neutralising antibodies (titres ≥ 45) against serotype O in the herds from Kween and Rakai districts, against SAT 1 in the herd from Nwoya district and against SAT 2 in the herds from Kiruhura, Isingiro and Ntungamo districts. The isolation of a SAT 2 FMDV from Isingiro was consistent with the detection of high levels of neutralising antibodies against SAT 2; sequencing (for the VP1 coding region) indicated that this virus belonged to lineage I within this serotype, like the currently used vaccine strain. From the Wakiso district 11 tissue/swab samples were collected; serotype A FMDV, genotype Africa (G-I), was isolated from the epithelial samples. This study shows that within a period of less than one year, FMD outbreaks in Uganda were caused by four different serotypes namely O, A, SAT 1 and SAT 2. Therefore, to enhance the control of FMD in Uganda, there is need for efficient and timely determination of outbreak virus strains/serotypes and vaccine matching. The value of incorporating serotype A antigen into the imported vaccines along with the current serotype O, SAT 1 and SAT 2 strains should be considered.

Show MeSH
Related in: MedlinePlus