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In vitro anti-proliferative activity of Argemone gracilenta and identification of some active components.

Leyva-Peralta MA, Robles-Zepeda RE, Garibay-Escobar A, Ruiz-Bustos E, Alvarez-Berber LP, Gálvez-Ruiz JC - BMC Complement Altern Med (2015)

Bottom Line: No activity was shown on the normal L-929 cell line.On the normal L-929 cell line, this mixture showed a range of IC50 values of 85.1 to 100 μg/mL.This is the first study that relates argemonine, berberine, and a mixture of fatty acids and fatty acid esters with the anti-proliferative activity displayed by Argemone gracilenta.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Boulevard Luis Encinas y Rosales s/n, Hermosillo, Sonora, 83000, Mexico. mario_ups@hotmail.com.

ABSTRACT

Background: Cancer is one of the leading causes of death worldwide. Natural products have been regarded as important sources of potential chemotherapeutic agents. In this study, we evaluated the anti-proliferative activity of Argemone gracilenta's methanol extract and its fractions. We identified those compounds of the most active fractions that displayed anti-proliferative activity.

Methods: The anti-proliferative activity on different cancerous cell lines (M12.C3F6, RAW 264.7, HeLa) was evaluated in vitro using the MTT colorimetric method. Identification of the active compounds present in the fractions with the highest activity was achieved by nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GC-MS) analyses.

Results: Both argemonine and berberine alkaloids, isolated from the ethyl acetate fraction, displayed high anti-proliferative activity with IC50 values of 2.8, 2.5, 12.1, and 2.7, 2.4, 79.5 μg/mL on M12.C3F6, RAW 264.7, and HeLa cancerous cell lines, respectively. No activity was shown on the normal L-929 cell line. From the hexane fraction, a mixture of fatty acids and fatty acid esters of 16 or more carbon atoms with anti-proliferative activity was identified, showing a range of IC50 values of 16.8-24.9, 34.1-35.4, and 67.6-91.8 μg/mL on M12.C3F6, RAW 264.7, and HeLa cancerous cell lines, respectively. On the normal L-929 cell line, this mixture showed a range of IC50 values of 85.1 to 100 μg/mL.

Conclusion: This is the first study that relates argemonine, berberine, and a mixture of fatty acids and fatty acid esters with the anti-proliferative activity displayed by Argemone gracilenta.

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Related in: MedlinePlus

Structure of the main component in Fag-7B fraction, berberine:1H NMR (13C NMR) data.
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Fig3: Structure of the main component in Fag-7B fraction, berberine:1H NMR (13C NMR) data.

Mentions: The compounds argemonine (7A [25] and berberine (7B [26]) were identified by comparing their spectroscopic data with those previously described in the literature (Figures 2 and 3). The purity of isolated compounds was determined to be above 95%, based on their 1H-NMR spectra.Figure 2


In vitro anti-proliferative activity of Argemone gracilenta and identification of some active components.

Leyva-Peralta MA, Robles-Zepeda RE, Garibay-Escobar A, Ruiz-Bustos E, Alvarez-Berber LP, Gálvez-Ruiz JC - BMC Complement Altern Med (2015)

Structure of the main component in Fag-7B fraction, berberine:1H NMR (13C NMR) data.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321710&req=5

Fig3: Structure of the main component in Fag-7B fraction, berberine:1H NMR (13C NMR) data.
Mentions: The compounds argemonine (7A [25] and berberine (7B [26]) were identified by comparing their spectroscopic data with those previously described in the literature (Figures 2 and 3). The purity of isolated compounds was determined to be above 95%, based on their 1H-NMR spectra.Figure 2

Bottom Line: No activity was shown on the normal L-929 cell line.On the normal L-929 cell line, this mixture showed a range of IC50 values of 85.1 to 100 μg/mL.This is the first study that relates argemonine, berberine, and a mixture of fatty acids and fatty acid esters with the anti-proliferative activity displayed by Argemone gracilenta.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Boulevard Luis Encinas y Rosales s/n, Hermosillo, Sonora, 83000, Mexico. mario_ups@hotmail.com.

ABSTRACT

Background: Cancer is one of the leading causes of death worldwide. Natural products have been regarded as important sources of potential chemotherapeutic agents. In this study, we evaluated the anti-proliferative activity of Argemone gracilenta's methanol extract and its fractions. We identified those compounds of the most active fractions that displayed anti-proliferative activity.

Methods: The anti-proliferative activity on different cancerous cell lines (M12.C3F6, RAW 264.7, HeLa) was evaluated in vitro using the MTT colorimetric method. Identification of the active compounds present in the fractions with the highest activity was achieved by nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GC-MS) analyses.

Results: Both argemonine and berberine alkaloids, isolated from the ethyl acetate fraction, displayed high anti-proliferative activity with IC50 values of 2.8, 2.5, 12.1, and 2.7, 2.4, 79.5 μg/mL on M12.C3F6, RAW 264.7, and HeLa cancerous cell lines, respectively. No activity was shown on the normal L-929 cell line. From the hexane fraction, a mixture of fatty acids and fatty acid esters of 16 or more carbon atoms with anti-proliferative activity was identified, showing a range of IC50 values of 16.8-24.9, 34.1-35.4, and 67.6-91.8 μg/mL on M12.C3F6, RAW 264.7, and HeLa cancerous cell lines, respectively. On the normal L-929 cell line, this mixture showed a range of IC50 values of 85.1 to 100 μg/mL.

Conclusion: This is the first study that relates argemonine, berberine, and a mixture of fatty acids and fatty acid esters with the anti-proliferative activity displayed by Argemone gracilenta.

Show MeSH
Related in: MedlinePlus