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In vitro anti-proliferative activity of Argemone gracilenta and identification of some active components.

Leyva-Peralta MA, Robles-Zepeda RE, Garibay-Escobar A, Ruiz-Bustos E, Alvarez-Berber LP, Gálvez-Ruiz JC - BMC Complement Altern Med (2015)

Bottom Line: No activity was shown on the normal L-929 cell line.On the normal L-929 cell line, this mixture showed a range of IC50 values of 85.1 to 100 μg/mL.This is the first study that relates argemonine, berberine, and a mixture of fatty acids and fatty acid esters with the anti-proliferative activity displayed by Argemone gracilenta.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Boulevard Luis Encinas y Rosales s/n, Hermosillo, Sonora, 83000, Mexico. mario_ups@hotmail.com.

ABSTRACT

Background: Cancer is one of the leading causes of death worldwide. Natural products have been regarded as important sources of potential chemotherapeutic agents. In this study, we evaluated the anti-proliferative activity of Argemone gracilenta's methanol extract and its fractions. We identified those compounds of the most active fractions that displayed anti-proliferative activity.

Methods: The anti-proliferative activity on different cancerous cell lines (M12.C3F6, RAW 264.7, HeLa) was evaluated in vitro using the MTT colorimetric method. Identification of the active compounds present in the fractions with the highest activity was achieved by nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GC-MS) analyses.

Results: Both argemonine and berberine alkaloids, isolated from the ethyl acetate fraction, displayed high anti-proliferative activity with IC50 values of 2.8, 2.5, 12.1, and 2.7, 2.4, 79.5 μg/mL on M12.C3F6, RAW 264.7, and HeLa cancerous cell lines, respectively. No activity was shown on the normal L-929 cell line. From the hexane fraction, a mixture of fatty acids and fatty acid esters of 16 or more carbon atoms with anti-proliferative activity was identified, showing a range of IC50 values of 16.8-24.9, 34.1-35.4, and 67.6-91.8 μg/mL on M12.C3F6, RAW 264.7, and HeLa cancerous cell lines, respectively. On the normal L-929 cell line, this mixture showed a range of IC50 values of 85.1 to 100 μg/mL.

Conclusion: This is the first study that relates argemonine, berberine, and a mixture of fatty acids and fatty acid esters with the anti-proliferative activity displayed by Argemone gracilenta.

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Related in: MedlinePlus

Anti-proliferative activity of the Fag-7A (argemonine) fraction on cell lines. A) M12.C3F6 and B) HeLa, at a concentration of 50 μg/mL. Observation at 48 h in inverted microscope. Morphological changes: condensed nucleus (CN), apoptotic bodies (AB), autophagic vacuoles (AV), condensed cytoplasm (CC). All images are magnified at 40×. Images shown are representative of at least four such fields of view per sample and three independent trials.
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Fig1: Anti-proliferative activity of the Fag-7A (argemonine) fraction on cell lines. A) M12.C3F6 and B) HeLa, at a concentration of 50 μg/mL. Observation at 48 h in inverted microscope. Morphological changes: condensed nucleus (CN), apoptotic bodies (AB), autophagic vacuoles (AV), condensed cytoplasm (CC). All images are magnified at 40×. Images shown are representative of at least four such fields of view per sample and three independent trials.

Mentions: The cell lines were observed 48 h after exposure to argemonine and evidence of two different cell death pathways was found. In the M12.C3F6 cell line (Figure 1A), autophagic vacuoles and degradation of cytoplasm contents, characteristic morphological changes of cell death by autophagy, were observed. In the HeLa cell line (Figure 1B), condensation of the nucleus and cytoplasm, and apoptotic body formation, characteristic morphological changes of cell death by apoptosis, were present.Figure 1


In vitro anti-proliferative activity of Argemone gracilenta and identification of some active components.

Leyva-Peralta MA, Robles-Zepeda RE, Garibay-Escobar A, Ruiz-Bustos E, Alvarez-Berber LP, Gálvez-Ruiz JC - BMC Complement Altern Med (2015)

Anti-proliferative activity of the Fag-7A (argemonine) fraction on cell lines. A) M12.C3F6 and B) HeLa, at a concentration of 50 μg/mL. Observation at 48 h in inverted microscope. Morphological changes: condensed nucleus (CN), apoptotic bodies (AB), autophagic vacuoles (AV), condensed cytoplasm (CC). All images are magnified at 40×. Images shown are representative of at least four such fields of view per sample and three independent trials.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321710&req=5

Fig1: Anti-proliferative activity of the Fag-7A (argemonine) fraction on cell lines. A) M12.C3F6 and B) HeLa, at a concentration of 50 μg/mL. Observation at 48 h in inverted microscope. Morphological changes: condensed nucleus (CN), apoptotic bodies (AB), autophagic vacuoles (AV), condensed cytoplasm (CC). All images are magnified at 40×. Images shown are representative of at least four such fields of view per sample and three independent trials.
Mentions: The cell lines were observed 48 h after exposure to argemonine and evidence of two different cell death pathways was found. In the M12.C3F6 cell line (Figure 1A), autophagic vacuoles and degradation of cytoplasm contents, characteristic morphological changes of cell death by autophagy, were observed. In the HeLa cell line (Figure 1B), condensation of the nucleus and cytoplasm, and apoptotic body formation, characteristic morphological changes of cell death by apoptosis, were present.Figure 1

Bottom Line: No activity was shown on the normal L-929 cell line.On the normal L-929 cell line, this mixture showed a range of IC50 values of 85.1 to 100 μg/mL.This is the first study that relates argemonine, berberine, and a mixture of fatty acids and fatty acid esters with the anti-proliferative activity displayed by Argemone gracilenta.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Boulevard Luis Encinas y Rosales s/n, Hermosillo, Sonora, 83000, Mexico. mario_ups@hotmail.com.

ABSTRACT

Background: Cancer is one of the leading causes of death worldwide. Natural products have been regarded as important sources of potential chemotherapeutic agents. In this study, we evaluated the anti-proliferative activity of Argemone gracilenta's methanol extract and its fractions. We identified those compounds of the most active fractions that displayed anti-proliferative activity.

Methods: The anti-proliferative activity on different cancerous cell lines (M12.C3F6, RAW 264.7, HeLa) was evaluated in vitro using the MTT colorimetric method. Identification of the active compounds present in the fractions with the highest activity was achieved by nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GC-MS) analyses.

Results: Both argemonine and berberine alkaloids, isolated from the ethyl acetate fraction, displayed high anti-proliferative activity with IC50 values of 2.8, 2.5, 12.1, and 2.7, 2.4, 79.5 μg/mL on M12.C3F6, RAW 264.7, and HeLa cancerous cell lines, respectively. No activity was shown on the normal L-929 cell line. From the hexane fraction, a mixture of fatty acids and fatty acid esters of 16 or more carbon atoms with anti-proliferative activity was identified, showing a range of IC50 values of 16.8-24.9, 34.1-35.4, and 67.6-91.8 μg/mL on M12.C3F6, RAW 264.7, and HeLa cancerous cell lines, respectively. On the normal L-929 cell line, this mixture showed a range of IC50 values of 85.1 to 100 μg/mL.

Conclusion: This is the first study that relates argemonine, berberine, and a mixture of fatty acids and fatty acid esters with the anti-proliferative activity displayed by Argemone gracilenta.

Show MeSH
Related in: MedlinePlus