Limits...
Effect of Tai Chi on mononuclear cell functions in patients with non-small cell lung cancer.

Liu J, Chen P, Wang R, Yuan Y, Wang X, Li C - BMC Complement Altern Med (2015)

Bottom Line: Patients (n = 27) were randomly divided into the control group (n = 13) and the TCC group (n = 14).Peripheral blood was collected and PBMCs isolated before and after the 16-week TCC, PBMC proliferation and co-culture of PBMCs with the NSCLC cell line A549 were performed for proliferation and cell cytolysis assays.Analysis of NKT cells, NK cells, and CD123+ and CD11c + dendritic cells were also performed. (1) After 16-week of TCC, cell proliferation increased significantly as compared with the control. (2) PBMCs from the TCC group also demonstrated enhanced cytolytic/oncolytic activity against A549 cells. (3) Significant differences were also found in NK cell percentage at t = 16 weeks, post-pre changes of NKT and DC11c between groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Martial Arts, Shanghai University of Sport, Shanghai, 200438, China. liujing_j3@163.com.

ABSTRACT

Background: Tai Chi is the Chinese traditional medicine exercise for mind-body health. The objective of this study is to investigate the effect of Tai Chi Chuan (TCC) exercise on the proliferative and cytolytic/tumoricidal activities of peripheral blood mononuclear cells (PBMCs) in postsurgical non-small cell lung cancer (NSCLC) patients.

Methods: Patients (n = 27) were randomly divided into the control group (n = 13) and the TCC group (n = 14). TCC group participated in Tai Chi 24-type exercise for 16 weeks, 60-min every time, and three times a week. Peripheral blood was collected and PBMCs isolated before and after the 16-week TCC, PBMC proliferation and co-culture of PBMCs with the NSCLC cell line A549 were performed for proliferation and cell cytolysis assays. Analysis of NKT cells, NK cells, and CD123+ and CD11c + dendritic cells were also performed.

Results: (1) After 16-week of TCC, cell proliferation increased significantly as compared with the control. (2) PBMCs from the TCC group also demonstrated enhanced cytolytic/oncolytic activity against A549 cells. (3) Significant differences were also found in NK cell percentage at t = 16 weeks, post-pre changes of NKT and DC11c between groups.

Conclusion: Regular Tai Chi exercise has the promise of enhancing PBMC proliferative and cytolytic activities in NSCLC patients. Our results affirm the value of a future trial with a larger scale and longer duration for cancer survivors.

Trial registration: ChiCTR-TRC-11001404 .

Show MeSH

Related in: MedlinePlus

Changes in mean NK, NKT, and DC cells in the Tai Chi group and the control group. The units of the change values (NK, NKT, DC123 and DC11c) are percentage of cells in CD3+ lymphocytes. The error bars represent SD of change values. * p < 0.05; ** p < 0.01 compared between TCC group and control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4321705&req=5

Fig2: Changes in mean NK, NKT, and DC cells in the Tai Chi group and the control group. The units of the change values (NK, NKT, DC123 and DC11c) are percentage of cells in CD3+ lymphocytes. The error bars represent SD of change values. * p < 0.05; ** p < 0.01 compared between TCC group and control group.

Mentions: All data were checked for normality using the Shapiro-Wilk W test in SPSS (version 15.0, for Windows). If data were not normally distributed, a natural logarithm transformation was applied. A sample sizes of 16 (8 subjects per group) achieved 80% power to detect an effect size of 0.626 using an F test with a 0.05 significance level. Data are presented as mean ± standard deviation, and independent (unpaired) samples t-test was used to assess differences between control and TCC group before and after intervention. Paired-samples t-test was also used to assess differences between variables pre- and post-intervention. In addition, for immune cells of each individual, pre-test values were subtracted from post-test values to obtain individual change values (see Figure 2). When outcome variables showed significant differences in group-by-time interaction, absolute change values were compared between groups using independent t tests. p < 0.05 was considered statistically significant.Figure 2


Effect of Tai Chi on mononuclear cell functions in patients with non-small cell lung cancer.

Liu J, Chen P, Wang R, Yuan Y, Wang X, Li C - BMC Complement Altern Med (2015)

Changes in mean NK, NKT, and DC cells in the Tai Chi group and the control group. The units of the change values (NK, NKT, DC123 and DC11c) are percentage of cells in CD3+ lymphocytes. The error bars represent SD of change values. * p < 0.05; ** p < 0.01 compared between TCC group and control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321705&req=5

Fig2: Changes in mean NK, NKT, and DC cells in the Tai Chi group and the control group. The units of the change values (NK, NKT, DC123 and DC11c) are percentage of cells in CD3+ lymphocytes. The error bars represent SD of change values. * p < 0.05; ** p < 0.01 compared between TCC group and control group.
Mentions: All data were checked for normality using the Shapiro-Wilk W test in SPSS (version 15.0, for Windows). If data were not normally distributed, a natural logarithm transformation was applied. A sample sizes of 16 (8 subjects per group) achieved 80% power to detect an effect size of 0.626 using an F test with a 0.05 significance level. Data are presented as mean ± standard deviation, and independent (unpaired) samples t-test was used to assess differences between control and TCC group before and after intervention. Paired-samples t-test was also used to assess differences between variables pre- and post-intervention. In addition, for immune cells of each individual, pre-test values were subtracted from post-test values to obtain individual change values (see Figure 2). When outcome variables showed significant differences in group-by-time interaction, absolute change values were compared between groups using independent t tests. p < 0.05 was considered statistically significant.Figure 2

Bottom Line: Patients (n = 27) were randomly divided into the control group (n = 13) and the TCC group (n = 14).Peripheral blood was collected and PBMCs isolated before and after the 16-week TCC, PBMC proliferation and co-culture of PBMCs with the NSCLC cell line A549 were performed for proliferation and cell cytolysis assays.Analysis of NKT cells, NK cells, and CD123+ and CD11c + dendritic cells were also performed. (1) After 16-week of TCC, cell proliferation increased significantly as compared with the control. (2) PBMCs from the TCC group also demonstrated enhanced cytolytic/oncolytic activity against A549 cells. (3) Significant differences were also found in NK cell percentage at t = 16 weeks, post-pre changes of NKT and DC11c between groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Martial Arts, Shanghai University of Sport, Shanghai, 200438, China. liujing_j3@163.com.

ABSTRACT

Background: Tai Chi is the Chinese traditional medicine exercise for mind-body health. The objective of this study is to investigate the effect of Tai Chi Chuan (TCC) exercise on the proliferative and cytolytic/tumoricidal activities of peripheral blood mononuclear cells (PBMCs) in postsurgical non-small cell lung cancer (NSCLC) patients.

Methods: Patients (n = 27) were randomly divided into the control group (n = 13) and the TCC group (n = 14). TCC group participated in Tai Chi 24-type exercise for 16 weeks, 60-min every time, and three times a week. Peripheral blood was collected and PBMCs isolated before and after the 16-week TCC, PBMC proliferation and co-culture of PBMCs with the NSCLC cell line A549 were performed for proliferation and cell cytolysis assays. Analysis of NKT cells, NK cells, and CD123+ and CD11c + dendritic cells were also performed.

Results: (1) After 16-week of TCC, cell proliferation increased significantly as compared with the control. (2) PBMCs from the TCC group also demonstrated enhanced cytolytic/oncolytic activity against A549 cells. (3) Significant differences were also found in NK cell percentage at t = 16 weeks, post-pre changes of NKT and DC11c between groups.

Conclusion: Regular Tai Chi exercise has the promise of enhancing PBMC proliferative and cytolytic activities in NSCLC patients. Our results affirm the value of a future trial with a larger scale and longer duration for cancer survivors.

Trial registration: ChiCTR-TRC-11001404 .

Show MeSH
Related in: MedlinePlus