Limits...
Hepatoprotective Effect of Terminalia chebula against t-BHP-Induced Acute Liver Injury in C57/BL6 Mice.

Choi MK, Kim HG, Han JM, Lee JS, Lee JS, Chung SH, Son CG - Evid Based Complement Alternat Med (2015)

Bottom Line: Inflammatory cytokines including TNF-α, IL-1β, and IL-6 were notably increased in hepatic tissues, and then these were efficiently attenuated by TCW pretreatment. t-BHP injection notably increased malondialdehyde, total reactive oxygen species, and nitric oxide in the liver tissue, while it markedly dropped the antioxidant activities including total antioxidant capacity, total glutathione contents, glutathione peroxidase, superoxide dismutase, and catalase.TCW pretreatment remarkably ameliorated these alterations, and these effects were relevant to gene expressions.Histopathological examinations supported the above findings.

View Article: PubMed Central - PubMed

Affiliation: Liver and Immunology Research Center, Daejeon Oriental Hospital of Daejeon University, 22-5 Daehung-dong, Jung-gu, Daejeon 301-724, Republic of Korea.

ABSTRACT
We aimed to identify the hepatoprotective effects of Terminalia chebula water extract (TCW) and its corresponding pharmacological actions using C57/BL6 mice model of tert-butylhydroperoxide-(t-BHP-) induced acute liver injury. Mice were orally administered with TCW (0, 50, 100, or 200 mg/kg) or gallic acid (100 mg/kg) for 5 days before t-BHP (2.5 mM/kg) injection. Liver enzymes, histopathology, oxidative stress parameters, antioxidant components, and inflammatory cytokines were examined 18 h after t-BHP injection. t-BHP injection caused dramatic elevation of serum AST, ALT, and LDH level, while TCW pretreatment notably attenuated these elevations. Inflammatory cytokines including TNF-α, IL-1β, and IL-6 were notably increased in hepatic tissues, and then these were efficiently attenuated by TCW pretreatment. t-BHP injection notably increased malondialdehyde, total reactive oxygen species, and nitric oxide in the liver tissue, while it markedly dropped the antioxidant activities including total antioxidant capacity, total glutathione contents, glutathione peroxidase, superoxide dismutase, and catalase. TCW pretreatment remarkably ameliorated these alterations, and these effects were relevant to gene expressions. Histopathological examinations supported the above findings. Collectively, these findings well prove that TCW beneficially prevents acute and severe liver injury and clarify its corresponding mechanisms involved in the inhibition of oxidative stress and inflammatory cytokines.

No MeSH data available.


Related in: MedlinePlus

Serum biochemistries and histopathological and immunohistochemical findings of hepatic tissue. The mice were administered with TCW (50, 100, and 200 mg/kg), gallic acid, or distilled water orally once per day for 5 days. After 18 h of t-BHP injection, serum AST (a), ALT (b), and LDH (c) levels were measured. H&E staining (d) and anti-4-HNE staining (e) were conducted and examined under microscopy (×200). Data are expressed as the mean ± SD (n = 6). #P < 0.05, ##P < 0.01, and ###P < 0.001 compared with the naive group; *P < 0.05 compared with the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4321673&req=5

fig2: Serum biochemistries and histopathological and immunohistochemical findings of hepatic tissue. The mice were administered with TCW (50, 100, and 200 mg/kg), gallic acid, or distilled water orally once per day for 5 days. After 18 h of t-BHP injection, serum AST (a), ALT (b), and LDH (c) levels were measured. H&E staining (d) and anti-4-HNE staining (e) were conducted and examined under microscopy (×200). Data are expressed as the mean ± SD (n = 6). #P < 0.05, ##P < 0.01, and ###P < 0.001 compared with the naive group; *P < 0.05 compared with the control group.

Mentions: t-BHP injection dramatically increased serum AST, ALT, and LDH levels approximately 41.7-, 62.2-, and 17.1-fold compared with naive group, whereas TCW pretreatment significantly attenuated these increases of both serum AST and ALT levels as compared with the control group, respectively (P < 0.05 for 200 mg/kg in AST; P < 0.05 for 100 and 200 mg/kg in ALT, Figures 2(a) and 2(b)). TCW pretreatment reduced serum LDH level but did not reach the statistical significance (Figure 2(c)). Gallic acid pretreatment showed similar effects as those of TCW.


Hepatoprotective Effect of Terminalia chebula against t-BHP-Induced Acute Liver Injury in C57/BL6 Mice.

Choi MK, Kim HG, Han JM, Lee JS, Lee JS, Chung SH, Son CG - Evid Based Complement Alternat Med (2015)

Serum biochemistries and histopathological and immunohistochemical findings of hepatic tissue. The mice were administered with TCW (50, 100, and 200 mg/kg), gallic acid, or distilled water orally once per day for 5 days. After 18 h of t-BHP injection, serum AST (a), ALT (b), and LDH (c) levels were measured. H&E staining (d) and anti-4-HNE staining (e) were conducted and examined under microscopy (×200). Data are expressed as the mean ± SD (n = 6). #P < 0.05, ##P < 0.01, and ###P < 0.001 compared with the naive group; *P < 0.05 compared with the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4321673&req=5

fig2: Serum biochemistries and histopathological and immunohistochemical findings of hepatic tissue. The mice were administered with TCW (50, 100, and 200 mg/kg), gallic acid, or distilled water orally once per day for 5 days. After 18 h of t-BHP injection, serum AST (a), ALT (b), and LDH (c) levels were measured. H&E staining (d) and anti-4-HNE staining (e) were conducted and examined under microscopy (×200). Data are expressed as the mean ± SD (n = 6). #P < 0.05, ##P < 0.01, and ###P < 0.001 compared with the naive group; *P < 0.05 compared with the control group.
Mentions: t-BHP injection dramatically increased serum AST, ALT, and LDH levels approximately 41.7-, 62.2-, and 17.1-fold compared with naive group, whereas TCW pretreatment significantly attenuated these increases of both serum AST and ALT levels as compared with the control group, respectively (P < 0.05 for 200 mg/kg in AST; P < 0.05 for 100 and 200 mg/kg in ALT, Figures 2(a) and 2(b)). TCW pretreatment reduced serum LDH level but did not reach the statistical significance (Figure 2(c)). Gallic acid pretreatment showed similar effects as those of TCW.

Bottom Line: Inflammatory cytokines including TNF-α, IL-1β, and IL-6 were notably increased in hepatic tissues, and then these were efficiently attenuated by TCW pretreatment. t-BHP injection notably increased malondialdehyde, total reactive oxygen species, and nitric oxide in the liver tissue, while it markedly dropped the antioxidant activities including total antioxidant capacity, total glutathione contents, glutathione peroxidase, superoxide dismutase, and catalase.TCW pretreatment remarkably ameliorated these alterations, and these effects were relevant to gene expressions.Histopathological examinations supported the above findings.

View Article: PubMed Central - PubMed

Affiliation: Liver and Immunology Research Center, Daejeon Oriental Hospital of Daejeon University, 22-5 Daehung-dong, Jung-gu, Daejeon 301-724, Republic of Korea.

ABSTRACT
We aimed to identify the hepatoprotective effects of Terminalia chebula water extract (TCW) and its corresponding pharmacological actions using C57/BL6 mice model of tert-butylhydroperoxide-(t-BHP-) induced acute liver injury. Mice were orally administered with TCW (0, 50, 100, or 200 mg/kg) or gallic acid (100 mg/kg) for 5 days before t-BHP (2.5 mM/kg) injection. Liver enzymes, histopathology, oxidative stress parameters, antioxidant components, and inflammatory cytokines were examined 18 h after t-BHP injection. t-BHP injection caused dramatic elevation of serum AST, ALT, and LDH level, while TCW pretreatment notably attenuated these elevations. Inflammatory cytokines including TNF-α, IL-1β, and IL-6 were notably increased in hepatic tissues, and then these were efficiently attenuated by TCW pretreatment. t-BHP injection notably increased malondialdehyde, total reactive oxygen species, and nitric oxide in the liver tissue, while it markedly dropped the antioxidant activities including total antioxidant capacity, total glutathione contents, glutathione peroxidase, superoxide dismutase, and catalase. TCW pretreatment remarkably ameliorated these alterations, and these effects were relevant to gene expressions. Histopathological examinations supported the above findings. Collectively, these findings well prove that TCW beneficially prevents acute and severe liver injury and clarify its corresponding mechanisms involved in the inhibition of oxidative stress and inflammatory cytokines.

No MeSH data available.


Related in: MedlinePlus