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Hepatoprotective Effect of Terminalia chebula against t-BHP-Induced Acute Liver Injury in C57/BL6 Mice.

Choi MK, Kim HG, Han JM, Lee JS, Lee JS, Chung SH, Son CG - Evid Based Complement Alternat Med (2015)

Bottom Line: Inflammatory cytokines including TNF-α, IL-1β, and IL-6 were notably increased in hepatic tissues, and then these were efficiently attenuated by TCW pretreatment. t-BHP injection notably increased malondialdehyde, total reactive oxygen species, and nitric oxide in the liver tissue, while it markedly dropped the antioxidant activities including total antioxidant capacity, total glutathione contents, glutathione peroxidase, superoxide dismutase, and catalase.TCW pretreatment remarkably ameliorated these alterations, and these effects were relevant to gene expressions.Histopathological examinations supported the above findings.

View Article: PubMed Central - PubMed

Affiliation: Liver and Immunology Research Center, Daejeon Oriental Hospital of Daejeon University, 22-5 Daehung-dong, Jung-gu, Daejeon 301-724, Republic of Korea.

ABSTRACT
We aimed to identify the hepatoprotective effects of Terminalia chebula water extract (TCW) and its corresponding pharmacological actions using C57/BL6 mice model of tert-butylhydroperoxide-(t-BHP-) induced acute liver injury. Mice were orally administered with TCW (0, 50, 100, or 200 mg/kg) or gallic acid (100 mg/kg) for 5 days before t-BHP (2.5 mM/kg) injection. Liver enzymes, histopathology, oxidative stress parameters, antioxidant components, and inflammatory cytokines were examined 18 h after t-BHP injection. t-BHP injection caused dramatic elevation of serum AST, ALT, and LDH level, while TCW pretreatment notably attenuated these elevations. Inflammatory cytokines including TNF-α, IL-1β, and IL-6 were notably increased in hepatic tissues, and then these were efficiently attenuated by TCW pretreatment. t-BHP injection notably increased malondialdehyde, total reactive oxygen species, and nitric oxide in the liver tissue, while it markedly dropped the antioxidant activities including total antioxidant capacity, total glutathione contents, glutathione peroxidase, superoxide dismutase, and catalase. TCW pretreatment remarkably ameliorated these alterations, and these effects were relevant to gene expressions. Histopathological examinations supported the above findings. Collectively, these findings well prove that TCW beneficially prevents acute and severe liver injury and clarify its corresponding mechanisms involved in the inhibition of oxidative stress and inflammatory cytokines.

No MeSH data available.


Related in: MedlinePlus

Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis of Terminalia chebula water extract (TCW). The TCW sample was subjected to UHPLC analysis (a), and seven main compounds were identified using an HPLC-MS database (b).
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fig1: Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis of Terminalia chebula water extract (TCW). The TCW sample was subjected to UHPLC analysis (a), and seven main compounds were identified using an HPLC-MS database (b).

Mentions: The chemical compounds from TCW were analyzed using UHPLC-MS/MS. A total of seven of the major peaks appeared at 1.31, 2.39, 5.33, 6.48, 6.89, 7.70, and 8.54 min of retention time, respectively (Figure 1(a)). From the chemical formulae analysis using high-resolution mass spectra, these compounds were identified as the chebulic acid (C14H12O11), gallic acid (C7H6O5), punicalagin (C48H28O30), geraniin (C41H28O27), phyllanemblinin E (C41H34O28), chebulagic acid (C41H30O27), and chebulinic acid (C41H32O27) (retention times at 1.31, 2.39, 5.33, 6.48, 6.89, 7.70, and 8.54, resp., Figure 1(b)). In addition, the quantitative analysis result showed that gallic acid content in TCW sample was approximately 1.69% (Supplementary Figure 1(c)).


Hepatoprotective Effect of Terminalia chebula against t-BHP-Induced Acute Liver Injury in C57/BL6 Mice.

Choi MK, Kim HG, Han JM, Lee JS, Lee JS, Chung SH, Son CG - Evid Based Complement Alternat Med (2015)

Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis of Terminalia chebula water extract (TCW). The TCW sample was subjected to UHPLC analysis (a), and seven main compounds were identified using an HPLC-MS database (b).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4321673&req=5

fig1: Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis of Terminalia chebula water extract (TCW). The TCW sample was subjected to UHPLC analysis (a), and seven main compounds were identified using an HPLC-MS database (b).
Mentions: The chemical compounds from TCW were analyzed using UHPLC-MS/MS. A total of seven of the major peaks appeared at 1.31, 2.39, 5.33, 6.48, 6.89, 7.70, and 8.54 min of retention time, respectively (Figure 1(a)). From the chemical formulae analysis using high-resolution mass spectra, these compounds were identified as the chebulic acid (C14H12O11), gallic acid (C7H6O5), punicalagin (C48H28O30), geraniin (C41H28O27), phyllanemblinin E (C41H34O28), chebulagic acid (C41H30O27), and chebulinic acid (C41H32O27) (retention times at 1.31, 2.39, 5.33, 6.48, 6.89, 7.70, and 8.54, resp., Figure 1(b)). In addition, the quantitative analysis result showed that gallic acid content in TCW sample was approximately 1.69% (Supplementary Figure 1(c)).

Bottom Line: Inflammatory cytokines including TNF-α, IL-1β, and IL-6 were notably increased in hepatic tissues, and then these were efficiently attenuated by TCW pretreatment. t-BHP injection notably increased malondialdehyde, total reactive oxygen species, and nitric oxide in the liver tissue, while it markedly dropped the antioxidant activities including total antioxidant capacity, total glutathione contents, glutathione peroxidase, superoxide dismutase, and catalase.TCW pretreatment remarkably ameliorated these alterations, and these effects were relevant to gene expressions.Histopathological examinations supported the above findings.

View Article: PubMed Central - PubMed

Affiliation: Liver and Immunology Research Center, Daejeon Oriental Hospital of Daejeon University, 22-5 Daehung-dong, Jung-gu, Daejeon 301-724, Republic of Korea.

ABSTRACT
We aimed to identify the hepatoprotective effects of Terminalia chebula water extract (TCW) and its corresponding pharmacological actions using C57/BL6 mice model of tert-butylhydroperoxide-(t-BHP-) induced acute liver injury. Mice were orally administered with TCW (0, 50, 100, or 200 mg/kg) or gallic acid (100 mg/kg) for 5 days before t-BHP (2.5 mM/kg) injection. Liver enzymes, histopathology, oxidative stress parameters, antioxidant components, and inflammatory cytokines were examined 18 h after t-BHP injection. t-BHP injection caused dramatic elevation of serum AST, ALT, and LDH level, while TCW pretreatment notably attenuated these elevations. Inflammatory cytokines including TNF-α, IL-1β, and IL-6 were notably increased in hepatic tissues, and then these were efficiently attenuated by TCW pretreatment. t-BHP injection notably increased malondialdehyde, total reactive oxygen species, and nitric oxide in the liver tissue, while it markedly dropped the antioxidant activities including total antioxidant capacity, total glutathione contents, glutathione peroxidase, superoxide dismutase, and catalase. TCW pretreatment remarkably ameliorated these alterations, and these effects were relevant to gene expressions. Histopathological examinations supported the above findings. Collectively, these findings well prove that TCW beneficially prevents acute and severe liver injury and clarify its corresponding mechanisms involved in the inhibition of oxidative stress and inflammatory cytokines.

No MeSH data available.


Related in: MedlinePlus