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In vivo DNA damaging and apoptotic potential of silver nanoparticles in Swiss albino mice.

Al Gurabi MA, Ali D, Alkahtani S, Alarifi S - Onco Targets Ther (2015)

Bottom Line: Nanoparticles can potentially cause adverse effects on organs, tissue, cell levels, and protein levels because of their physicochemical properties.AgNPs induced a significant increase in serum liver injury markers including alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase.Immunohistochemical and ultrastructural of AgNP.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.

ABSTRACT
Nanoparticles can potentially cause adverse effects on organs, tissue, cell levels, and protein levels because of their physicochemical properties. Silver nanoparticles (AgNPs) are being used on a wide scale in world consumer markets; their potential hazards for humans remain largely unknown. This study aimed to investigate the intraperitoneal toxicity of AgNPs (26 mg per kg of body weight, 52 mg per kg of body weight, and 78 mg per kg of body weight) over 72 hours in Swiss albino mice. AgNPs induced a significant increase in serum liver injury markers including alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase. Induction of DNA damage was also studied in mice injected with AgNPs. Apoptosis (detected by using the terminal deoxynucleotidyl transferase deoxyuridine triphosphatase nick end labeling assay method) in liver tissue and DNA strand breaks (detected by using the comet assay method) in lymphocytes revealed that a concentration of 78 mg of AgNPs per kg body weight can cause significant apoptosis and DNA damage. The DNA damage and apoptosis raise the concern about the safety associated with application of the AgNPs. Significantly more alterations were induced in the hepatocytes of animals exposed to AgNP doses than in the control animals. The induced histological and apoptotic changes may be due to AgNP toxicity. Immunohistochemical and ultrastructural of AgNP.

No MeSH data available.


Related in: MedlinePlus

Levels of enzymes that indicate liver function after exposure to AgNPs.Notes: Levels of ALP (A), ALT (B), and AST (C) in liver tissue samples of Swiss albino mice after exposure to AgNPs for 24 hours and for 72 hours. Each value represents the mean ± SEM of three experiments. *P<0.05 indicates a statistically significant higher concentration of substance in mice exposed to AgNPs than in control mice.Abbreviations: ALP, alkaline phosphatase; ALT, alanine aminotransferase; AST, aspartate aminotransferase; IU, international units; bw, body weight; AgNPs, silver nanoparticles; SEM, standard error of mean.
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f3-ott-8-295: Levels of enzymes that indicate liver function after exposure to AgNPs.Notes: Levels of ALP (A), ALT (B), and AST (C) in liver tissue samples of Swiss albino mice after exposure to AgNPs for 24 hours and for 72 hours. Each value represents the mean ± SEM of three experiments. *P<0.05 indicates a statistically significant higher concentration of substance in mice exposed to AgNPs than in control mice.Abbreviations: ALP, alkaline phosphatase; ALT, alanine aminotransferase; AST, aspartate aminotransferase; IU, international units; bw, body weight; AgNPs, silver nanoparticles; SEM, standard error of mean.

Mentions: The main outcome of this study was that significantly greater levels (P>0.05) of ALP, ALT, and AST were seen in mice treated with 26 mg AgNPs per kg body weight, 52 mg AgNPs per kg body weight, and 78 mg AgNPs per kg body weight than were seen in the control mice. The comparison between the mice exposed to AgNPs and those used as controls is shown in Figure 3. An initial step in measuring liver damage is a simple blood biochemical test to evaluate the presence of certain liver enzymes in the blood. The activity of these enzymes is normally used to determine liver function. Under normal conditions, these enzymes reside within the hepatocytes. But when the liver is injured, these enzymes are spilled in to blood stream.


In vivo DNA damaging and apoptotic potential of silver nanoparticles in Swiss albino mice.

Al Gurabi MA, Ali D, Alkahtani S, Alarifi S - Onco Targets Ther (2015)

Levels of enzymes that indicate liver function after exposure to AgNPs.Notes: Levels of ALP (A), ALT (B), and AST (C) in liver tissue samples of Swiss albino mice after exposure to AgNPs for 24 hours and for 72 hours. Each value represents the mean ± SEM of three experiments. *P<0.05 indicates a statistically significant higher concentration of substance in mice exposed to AgNPs than in control mice.Abbreviations: ALP, alkaline phosphatase; ALT, alanine aminotransferase; AST, aspartate aminotransferase; IU, international units; bw, body weight; AgNPs, silver nanoparticles; SEM, standard error of mean.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321656&req=5

f3-ott-8-295: Levels of enzymes that indicate liver function after exposure to AgNPs.Notes: Levels of ALP (A), ALT (B), and AST (C) in liver tissue samples of Swiss albino mice after exposure to AgNPs for 24 hours and for 72 hours. Each value represents the mean ± SEM of three experiments. *P<0.05 indicates a statistically significant higher concentration of substance in mice exposed to AgNPs than in control mice.Abbreviations: ALP, alkaline phosphatase; ALT, alanine aminotransferase; AST, aspartate aminotransferase; IU, international units; bw, body weight; AgNPs, silver nanoparticles; SEM, standard error of mean.
Mentions: The main outcome of this study was that significantly greater levels (P>0.05) of ALP, ALT, and AST were seen in mice treated with 26 mg AgNPs per kg body weight, 52 mg AgNPs per kg body weight, and 78 mg AgNPs per kg body weight than were seen in the control mice. The comparison between the mice exposed to AgNPs and those used as controls is shown in Figure 3. An initial step in measuring liver damage is a simple blood biochemical test to evaluate the presence of certain liver enzymes in the blood. The activity of these enzymes is normally used to determine liver function. Under normal conditions, these enzymes reside within the hepatocytes. But when the liver is injured, these enzymes are spilled in to blood stream.

Bottom Line: Nanoparticles can potentially cause adverse effects on organs, tissue, cell levels, and protein levels because of their physicochemical properties.AgNPs induced a significant increase in serum liver injury markers including alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase.Immunohistochemical and ultrastructural of AgNP.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.

ABSTRACT
Nanoparticles can potentially cause adverse effects on organs, tissue, cell levels, and protein levels because of their physicochemical properties. Silver nanoparticles (AgNPs) are being used on a wide scale in world consumer markets; their potential hazards for humans remain largely unknown. This study aimed to investigate the intraperitoneal toxicity of AgNPs (26 mg per kg of body weight, 52 mg per kg of body weight, and 78 mg per kg of body weight) over 72 hours in Swiss albino mice. AgNPs induced a significant increase in serum liver injury markers including alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase. Induction of DNA damage was also studied in mice injected with AgNPs. Apoptosis (detected by using the terminal deoxynucleotidyl transferase deoxyuridine triphosphatase nick end labeling assay method) in liver tissue and DNA strand breaks (detected by using the comet assay method) in lymphocytes revealed that a concentration of 78 mg of AgNPs per kg body weight can cause significant apoptosis and DNA damage. The DNA damage and apoptosis raise the concern about the safety associated with application of the AgNPs. Significantly more alterations were induced in the hepatocytes of animals exposed to AgNP doses than in the control animals. The induced histological and apoptotic changes may be due to AgNP toxicity. Immunohistochemical and ultrastructural of AgNP.

No MeSH data available.


Related in: MedlinePlus