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In vivo DNA damaging and apoptotic potential of silver nanoparticles in Swiss albino mice.

Al Gurabi MA, Ali D, Alkahtani S, Alarifi S - Onco Targets Ther (2015)

Bottom Line: Nanoparticles can potentially cause adverse effects on organs, tissue, cell levels, and protein levels because of their physicochemical properties.AgNPs induced a significant increase in serum liver injury markers including alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase.Immunohistochemical and ultrastructural of AgNP.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.

ABSTRACT
Nanoparticles can potentially cause adverse effects on organs, tissue, cell levels, and protein levels because of their physicochemical properties. Silver nanoparticles (AgNPs) are being used on a wide scale in world consumer markets; their potential hazards for humans remain largely unknown. This study aimed to investigate the intraperitoneal toxicity of AgNPs (26 mg per kg of body weight, 52 mg per kg of body weight, and 78 mg per kg of body weight) over 72 hours in Swiss albino mice. AgNPs induced a significant increase in serum liver injury markers including alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase. Induction of DNA damage was also studied in mice injected with AgNPs. Apoptosis (detected by using the terminal deoxynucleotidyl transferase deoxyuridine triphosphatase nick end labeling assay method) in liver tissue and DNA strand breaks (detected by using the comet assay method) in lymphocytes revealed that a concentration of 78 mg of AgNPs per kg body weight can cause significant apoptosis and DNA damage. The DNA damage and apoptosis raise the concern about the safety associated with application of the AgNPs. Significantly more alterations were induced in the hepatocytes of animals exposed to AgNP doses than in the control animals. The induced histological and apoptotic changes may be due to AgNP toxicity. Immunohistochemical and ultrastructural of AgNP.

No MeSH data available.


Related in: MedlinePlus

Characterization of the AgNPs.Notes: TEM image of the particles (A). The size distribution histogram generated by using a TEM image (B).Abbreviations: AgNPs, silver nanoparticles; TEM, transmission electron microscopy.
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f2-ott-8-295: Characterization of the AgNPs.Notes: TEM image of the particles (A). The size distribution histogram generated by using a TEM image (B).Abbreviations: AgNPs, silver nanoparticles; TEM, transmission electron microscopy.

Mentions: The size and shape of AgNPs were seen by TEM and observed in nano range, but the AgNPs made small agglomerates in aqueous suspension. The average size measured by TEM was 43.60±6.40 nm (Figure 2B). AgNPs had a hydrodynamic size of around 189 nm, and their zeta potential was -23.8 mV. The physicochemical effects of nanoparticles come from their high surface area to volume ratio. Nanoparticles have a higher fraction of atoms on their surfaces than do microparticles; this property makes nanoparticles more reactive.


In vivo DNA damaging and apoptotic potential of silver nanoparticles in Swiss albino mice.

Al Gurabi MA, Ali D, Alkahtani S, Alarifi S - Onco Targets Ther (2015)

Characterization of the AgNPs.Notes: TEM image of the particles (A). The size distribution histogram generated by using a TEM image (B).Abbreviations: AgNPs, silver nanoparticles; TEM, transmission electron microscopy.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321656&req=5

f2-ott-8-295: Characterization of the AgNPs.Notes: TEM image of the particles (A). The size distribution histogram generated by using a TEM image (B).Abbreviations: AgNPs, silver nanoparticles; TEM, transmission electron microscopy.
Mentions: The size and shape of AgNPs were seen by TEM and observed in nano range, but the AgNPs made small agglomerates in aqueous suspension. The average size measured by TEM was 43.60±6.40 nm (Figure 2B). AgNPs had a hydrodynamic size of around 189 nm, and their zeta potential was -23.8 mV. The physicochemical effects of nanoparticles come from their high surface area to volume ratio. Nanoparticles have a higher fraction of atoms on their surfaces than do microparticles; this property makes nanoparticles more reactive.

Bottom Line: Nanoparticles can potentially cause adverse effects on organs, tissue, cell levels, and protein levels because of their physicochemical properties.AgNPs induced a significant increase in serum liver injury markers including alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase.Immunohistochemical and ultrastructural of AgNP.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.

ABSTRACT
Nanoparticles can potentially cause adverse effects on organs, tissue, cell levels, and protein levels because of their physicochemical properties. Silver nanoparticles (AgNPs) are being used on a wide scale in world consumer markets; their potential hazards for humans remain largely unknown. This study aimed to investigate the intraperitoneal toxicity of AgNPs (26 mg per kg of body weight, 52 mg per kg of body weight, and 78 mg per kg of body weight) over 72 hours in Swiss albino mice. AgNPs induced a significant increase in serum liver injury markers including alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase. Induction of DNA damage was also studied in mice injected with AgNPs. Apoptosis (detected by using the terminal deoxynucleotidyl transferase deoxyuridine triphosphatase nick end labeling assay method) in liver tissue and DNA strand breaks (detected by using the comet assay method) in lymphocytes revealed that a concentration of 78 mg of AgNPs per kg body weight can cause significant apoptosis and DNA damage. The DNA damage and apoptosis raise the concern about the safety associated with application of the AgNPs. Significantly more alterations were induced in the hepatocytes of animals exposed to AgNP doses than in the control animals. The induced histological and apoptotic changes may be due to AgNP toxicity. Immunohistochemical and ultrastructural of AgNP.

No MeSH data available.


Related in: MedlinePlus