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Tubeimoside-1 induces glioma apoptosis through regulation of Bax/Bcl-2 and the ROS/Cytochrome C/Caspase-3 pathway.

Jia G, Wang Q, Wang R, Deng D, Xue L, Shao N, Zhang Y, Xia X, Zhi F, Yang Y - Onco Targets Ther (2015)

Bottom Line: Tubeimoside-1 (TBMS1) is a natural compound isolated from tubeimoside, which has been widely used as a traditional Chinese herbal medicine.Western blotting showed that TBMS1 induced apoptosis by increasing the expression of Bax and downregulating the level of Bcl-2.Furthermore, we found that TBMS1 induced apoptosis by increasing the concentration of reactive oxygen species through the release of Cytochrome C and activation of Caspase-3.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Third Affiliated Hospital of Soochow University, Jiangsu, People's Republic of China.

ABSTRACT

Background: Tubeimoside-1 (TBMS1) is a natural compound isolated from tubeimoside, which has been widely used as a traditional Chinese herbal medicine. The purpose of the present study is to investigate the anti-tumor effect and the underling mechanism of TBMS1 on glioma cancer cells.

Methods: The MTT assay was performed to evaluate the effect of TBMS1 on glioma cell proliferation. The fluorescent microscopy and flow cytometry analysis were performed to evaluate the effect of TBMS1 on glioma cell apoptosis. The Western blot analysis was used to evaluate the protein change.

Results: TBMS1 inhibited glioma cancer cell proliferation in a dose- and time-dependent manner. Fluorescent microscopy and flow cytometry analysis demonstrated that TBMS1 induced glioma cell apoptosis in a concentration-dependent manner. Western blotting showed that TBMS1 induced apoptosis by increasing the expression of Bax and downregulating the level of Bcl-2. Furthermore, we found that TBMS1 induced apoptosis by increasing the concentration of reactive oxygen species through the release of Cytochrome C and activation of Caspase-3.

Conclusion: These findings indicate that TBMS1 may be developed as a possible therapeutic agent for the management of glioma.

No MeSH data available.


Related in: MedlinePlus

Effects of TBMS1 on cell viability.Notes: U251 cells were treated with various concentrations of TBMS1 for 24, 48, and 72 hours. Data are expressed as a mean ± SD of three independent experiments, *P<0.05, #P<0.01, &P<0.001 compared to the control group.Abbreviations: TBMS1, Tubeimoside-1; SD, standard deviation; h, hours.
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f1-ott-8-303: Effects of TBMS1 on cell viability.Notes: U251 cells were treated with various concentrations of TBMS1 for 24, 48, and 72 hours. Data are expressed as a mean ± SD of three independent experiments, *P<0.05, #P<0.01, &P<0.001 compared to the control group.Abbreviations: TBMS1, Tubeimoside-1; SD, standard deviation; h, hours.

Mentions: U251 cells were treated with different concentrations of TBMS1 for 24, 48 or 72 hours, and then the survival rates were determined via MTT assay. As shown in Figure 1, TBMS1 inhibited the proliferation of U251 cells in a concentration- and time-dependent manner. Based on our data and the MTT results, we chose to use concentrations of 15, 20, and 25 μg/mL to investigate the mechanism of TBMS1 induced apoptosis in glioma cells. Furthermore, U87 cells were treated with different concentrations of TBMS1 for 24 hours, and then the survival rates were determined. As shown in Figure S1, TBMS1 inhibited the proliferation of U87 cells in a concentration-dependent manner which was similar to U251. However, the IC50 value of U87 cells was slightly higher than that of U251 cells, indicating that U87 cells were more resistant to TBMS1 treatment.


Tubeimoside-1 induces glioma apoptosis through regulation of Bax/Bcl-2 and the ROS/Cytochrome C/Caspase-3 pathway.

Jia G, Wang Q, Wang R, Deng D, Xue L, Shao N, Zhang Y, Xia X, Zhi F, Yang Y - Onco Targets Ther (2015)

Effects of TBMS1 on cell viability.Notes: U251 cells were treated with various concentrations of TBMS1 for 24, 48, and 72 hours. Data are expressed as a mean ± SD of three independent experiments, *P<0.05, #P<0.01, &P<0.001 compared to the control group.Abbreviations: TBMS1, Tubeimoside-1; SD, standard deviation; h, hours.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321652&req=5

f1-ott-8-303: Effects of TBMS1 on cell viability.Notes: U251 cells were treated with various concentrations of TBMS1 for 24, 48, and 72 hours. Data are expressed as a mean ± SD of three independent experiments, *P<0.05, #P<0.01, &P<0.001 compared to the control group.Abbreviations: TBMS1, Tubeimoside-1; SD, standard deviation; h, hours.
Mentions: U251 cells were treated with different concentrations of TBMS1 for 24, 48 or 72 hours, and then the survival rates were determined via MTT assay. As shown in Figure 1, TBMS1 inhibited the proliferation of U251 cells in a concentration- and time-dependent manner. Based on our data and the MTT results, we chose to use concentrations of 15, 20, and 25 μg/mL to investigate the mechanism of TBMS1 induced apoptosis in glioma cells. Furthermore, U87 cells were treated with different concentrations of TBMS1 for 24 hours, and then the survival rates were determined. As shown in Figure S1, TBMS1 inhibited the proliferation of U87 cells in a concentration-dependent manner which was similar to U251. However, the IC50 value of U87 cells was slightly higher than that of U251 cells, indicating that U87 cells were more resistant to TBMS1 treatment.

Bottom Line: Tubeimoside-1 (TBMS1) is a natural compound isolated from tubeimoside, which has been widely used as a traditional Chinese herbal medicine.Western blotting showed that TBMS1 induced apoptosis by increasing the expression of Bax and downregulating the level of Bcl-2.Furthermore, we found that TBMS1 induced apoptosis by increasing the concentration of reactive oxygen species through the release of Cytochrome C and activation of Caspase-3.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Third Affiliated Hospital of Soochow University, Jiangsu, People's Republic of China.

ABSTRACT

Background: Tubeimoside-1 (TBMS1) is a natural compound isolated from tubeimoside, which has been widely used as a traditional Chinese herbal medicine. The purpose of the present study is to investigate the anti-tumor effect and the underling mechanism of TBMS1 on glioma cancer cells.

Methods: The MTT assay was performed to evaluate the effect of TBMS1 on glioma cell proliferation. The fluorescent microscopy and flow cytometry analysis were performed to evaluate the effect of TBMS1 on glioma cell apoptosis. The Western blot analysis was used to evaluate the protein change.

Results: TBMS1 inhibited glioma cancer cell proliferation in a dose- and time-dependent manner. Fluorescent microscopy and flow cytometry analysis demonstrated that TBMS1 induced glioma cell apoptosis in a concentration-dependent manner. Western blotting showed that TBMS1 induced apoptosis by increasing the expression of Bax and downregulating the level of Bcl-2. Furthermore, we found that TBMS1 induced apoptosis by increasing the concentration of reactive oxygen species through the release of Cytochrome C and activation of Caspase-3.

Conclusion: These findings indicate that TBMS1 may be developed as a possible therapeutic agent for the management of glioma.

No MeSH data available.


Related in: MedlinePlus