Limits...
In vitro cytotoxicity analysis of doxorubicin-loaded/superparamagnetic iron oxide colloidal nanoassemblies on MCF7 and NIH3T3 cell lines.

Tomankova K, Polakova K, Pizova K, Binder S, Havrdova M, Kolarova M, Kriegova E, Zapletalova J, Malina L, Horakova J, Malohlava J, Kolokithas-Ntoukas A, Bakandritsos A, Kolarova H, Zboril R - Int J Nanomedicine (2015)

Bottom Line: For proper analysis and understanding of cell behavior after administration of MagAlg-DOX compared with free DOX, a complex set of in vitro tests, including production of reactive oxygen species, comet assay, cell cycle determination, gene expression, and cellular uptake, were utilized.It was found that the cytotoxic effect of MagAlg-DOX system is delayed compared to free DOX in both cell lines.We discovered that nanoparticles can attenuate or even inhibit the effect of DOX, particularly in the tumor MCF7 cell line.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biophysics, Institute of Translation Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.

ABSTRACT
One of the promising strategies for improvement of cancer treatment is based on magnetic drug delivery systems, thus avoiding side effects of standard chemotherapies. Superparamagnetic iron oxide (SPIO) nanoparticles have ideal properties to become a targeted magnetic drug delivery contrast probes, named theranostics. We worked with SPIO condensed colloidal nanocrystal clusters (MagAlg) prepared through a new soft biomineralization route in the presence of alginate as the polymeric shell and loaded with doxorubicin (DOX). The aim of this work was to study the in vitro cytotoxicity of these new MagAlg-DOX systems on mouse fibroblast and breast carcinoma cell lines. For proper analysis and understanding of cell behavior after administration of MagAlg-DOX compared with free DOX, a complex set of in vitro tests, including production of reactive oxygen species, comet assay, cell cycle determination, gene expression, and cellular uptake, were utilized. It was found that the cytotoxic effect of MagAlg-DOX system is delayed compared to free DOX in both cell lines. This was attributed to the different mechanism of internalization of DOX and MagAlg-DOX into the cells, together with the fact that the drug is strongly bound on the drug nanocarriers. We discovered that nanoparticles can attenuate or even inhibit the effect of DOX, particularly in the tumor MCF7 cell line. This is a first comprehensive study on the cytotoxic effect of DOX-loaded SPIO compared with free DOX on healthy and cancer cell lines, as well as on the induced changes in gene expression.

No MeSH data available.


Related in: MedlinePlus

The influence of DOX and MagAlg–DOX nanocarrier in concentration of 5 μM, 0.5 μM, and 0 μM on phosphorylation of histone H3 in MCF7 and NIH3T3 cell lines.Notes: Data represent mean and standard error from three independent measurements. Negative (•) significance were determined using Fisher’s exact test with Bonferroni correction for multiple comparisons.Abbreviation: DOX, doxorubicin.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4321606&req=5

f6-ijn-10-949: The influence of DOX and MagAlg–DOX nanocarrier in concentration of 5 μM, 0.5 μM, and 0 μM on phosphorylation of histone H3 in MCF7 and NIH3T3 cell lines.Notes: Data represent mean and standard error from three independent measurements. Negative (•) significance were determined using Fisher’s exact test with Bonferroni correction for multiple comparisons.Abbreviation: DOX, doxorubicin.

Mentions: The cells for the free-DOX and the MagAlg–DOX groups showed significant decrease in phosphorylation of histone H3 at all concentrations when compared with the control group in both the cell lines. Greater decrease in phosphorylation of histone H3 was observed for free DOX than for MagAlg–DOX (Figure 6).


In vitro cytotoxicity analysis of doxorubicin-loaded/superparamagnetic iron oxide colloidal nanoassemblies on MCF7 and NIH3T3 cell lines.

Tomankova K, Polakova K, Pizova K, Binder S, Havrdova M, Kolarova M, Kriegova E, Zapletalova J, Malina L, Horakova J, Malohlava J, Kolokithas-Ntoukas A, Bakandritsos A, Kolarova H, Zboril R - Int J Nanomedicine (2015)

The influence of DOX and MagAlg–DOX nanocarrier in concentration of 5 μM, 0.5 μM, and 0 μM on phosphorylation of histone H3 in MCF7 and NIH3T3 cell lines.Notes: Data represent mean and standard error from three independent measurements. Negative (•) significance were determined using Fisher’s exact test with Bonferroni correction for multiple comparisons.Abbreviation: DOX, doxorubicin.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321606&req=5

f6-ijn-10-949: The influence of DOX and MagAlg–DOX nanocarrier in concentration of 5 μM, 0.5 μM, and 0 μM on phosphorylation of histone H3 in MCF7 and NIH3T3 cell lines.Notes: Data represent mean and standard error from three independent measurements. Negative (•) significance were determined using Fisher’s exact test with Bonferroni correction for multiple comparisons.Abbreviation: DOX, doxorubicin.
Mentions: The cells for the free-DOX and the MagAlg–DOX groups showed significant decrease in phosphorylation of histone H3 at all concentrations when compared with the control group in both the cell lines. Greater decrease in phosphorylation of histone H3 was observed for free DOX than for MagAlg–DOX (Figure 6).

Bottom Line: For proper analysis and understanding of cell behavior after administration of MagAlg-DOX compared with free DOX, a complex set of in vitro tests, including production of reactive oxygen species, comet assay, cell cycle determination, gene expression, and cellular uptake, were utilized.It was found that the cytotoxic effect of MagAlg-DOX system is delayed compared to free DOX in both cell lines.We discovered that nanoparticles can attenuate or even inhibit the effect of DOX, particularly in the tumor MCF7 cell line.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biophysics, Institute of Translation Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.

ABSTRACT
One of the promising strategies for improvement of cancer treatment is based on magnetic drug delivery systems, thus avoiding side effects of standard chemotherapies. Superparamagnetic iron oxide (SPIO) nanoparticles have ideal properties to become a targeted magnetic drug delivery contrast probes, named theranostics. We worked with SPIO condensed colloidal nanocrystal clusters (MagAlg) prepared through a new soft biomineralization route in the presence of alginate as the polymeric shell and loaded with doxorubicin (DOX). The aim of this work was to study the in vitro cytotoxicity of these new MagAlg-DOX systems on mouse fibroblast and breast carcinoma cell lines. For proper analysis and understanding of cell behavior after administration of MagAlg-DOX compared with free DOX, a complex set of in vitro tests, including production of reactive oxygen species, comet assay, cell cycle determination, gene expression, and cellular uptake, were utilized. It was found that the cytotoxic effect of MagAlg-DOX system is delayed compared to free DOX in both cell lines. This was attributed to the different mechanism of internalization of DOX and MagAlg-DOX into the cells, together with the fact that the drug is strongly bound on the drug nanocarriers. We discovered that nanoparticles can attenuate or even inhibit the effect of DOX, particularly in the tumor MCF7 cell line. This is a first comprehensive study on the cytotoxic effect of DOX-loaded SPIO compared with free DOX on healthy and cancer cell lines, as well as on the induced changes in gene expression.

No MeSH data available.


Related in: MedlinePlus