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Sustained release of vancomycin from novel biodegradable nanofiber-loaded vascular prosthetic grafts: in vitro and in vivo study.

Liu KS, Lee CH, Wang YC, Liu SJ - Int J Nanomedicine (2015)

Bottom Line: Biodegradable nanofibers were prepared by first dissolving poly(D,L)-lactide-co-glycolide and vancomycin in 1,1,1,3,3,3-hexafluoro-2-propanol.The solution was then electrospun into nanofibers onto the surface of vascular prostheses.The in vitro release rates of the pharmaceutical from the nanofiber-loaded prostheses was characterized using an elution method and a high-performance liquid chromatography assay.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan.

ABSTRACT
This study describes novel biodegradable, drug-eluting nanofiber-loaded vascular prosthetic grafts that provide local and sustained delivery of vancomycin to surrounding tissues. Biodegradable nanofibers were prepared by first dissolving poly(D,L)-lactide-co-glycolide and vancomycin in 1,1,1,3,3,3-hexafluoro-2-propanol. The solution was then electrospun into nanofibers onto the surface of vascular prostheses. The in vitro release rates of the pharmaceutical from the nanofiber-loaded prostheses was characterized using an elution method and a high-performance liquid chromatography assay. Experimental results indicated that the drug-eluting prosthetic grafts released high concentrations of vancomycin in vitro (well above the minimum inhibitory concentration) for more than 30 days. In addition, the in vivo release behavior of the drug-eluting grafts implanted in the subcutaneous pocket of rabbits was also documented. The drug-eluting grafts developed in this work have potential applications in assisting the treatment of vascular prosthesis infection and resisting reinfection when an infected graft is to be exchanged.

No MeSH data available.


Related in: MedlinePlus

SEM photograph of electrospun drug-eluting nanofibers.Abbreviation: SEM, scanning electron microscope.
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f3-ijn-10-885: SEM photograph of electrospun drug-eluting nanofibers.Abbreviation: SEM, scanning electron microscope.

Mentions: In this study, by adopting appropriate process parameters (eg, solvent, polymer concentration, and flow rate), vancomycin-loaded vascular prosthetic grafts were successfully fabricated using the electrospinning procedure (Figure 2). Figure 3 shows the SEM micrographs of the electrospun drug-eluting nanofibers (under a magnification of 3,000×). The diameters of the spun PLGA/vancomycin nanofibers ranged from 70 to 1,200 nm. Additionally, the pore size of the nanofiber surface was approximately 5–10 μm.


Sustained release of vancomycin from novel biodegradable nanofiber-loaded vascular prosthetic grafts: in vitro and in vivo study.

Liu KS, Lee CH, Wang YC, Liu SJ - Int J Nanomedicine (2015)

SEM photograph of electrospun drug-eluting nanofibers.Abbreviation: SEM, scanning electron microscope.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321605&req=5

f3-ijn-10-885: SEM photograph of electrospun drug-eluting nanofibers.Abbreviation: SEM, scanning electron microscope.
Mentions: In this study, by adopting appropriate process parameters (eg, solvent, polymer concentration, and flow rate), vancomycin-loaded vascular prosthetic grafts were successfully fabricated using the electrospinning procedure (Figure 2). Figure 3 shows the SEM micrographs of the electrospun drug-eluting nanofibers (under a magnification of 3,000×). The diameters of the spun PLGA/vancomycin nanofibers ranged from 70 to 1,200 nm. Additionally, the pore size of the nanofiber surface was approximately 5–10 μm.

Bottom Line: Biodegradable nanofibers were prepared by first dissolving poly(D,L)-lactide-co-glycolide and vancomycin in 1,1,1,3,3,3-hexafluoro-2-propanol.The solution was then electrospun into nanofibers onto the surface of vascular prostheses.The in vitro release rates of the pharmaceutical from the nanofiber-loaded prostheses was characterized using an elution method and a high-performance liquid chromatography assay.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan.

ABSTRACT
This study describes novel biodegradable, drug-eluting nanofiber-loaded vascular prosthetic grafts that provide local and sustained delivery of vancomycin to surrounding tissues. Biodegradable nanofibers were prepared by first dissolving poly(D,L)-lactide-co-glycolide and vancomycin in 1,1,1,3,3,3-hexafluoro-2-propanol. The solution was then electrospun into nanofibers onto the surface of vascular prostheses. The in vitro release rates of the pharmaceutical from the nanofiber-loaded prostheses was characterized using an elution method and a high-performance liquid chromatography assay. Experimental results indicated that the drug-eluting prosthetic grafts released high concentrations of vancomycin in vitro (well above the minimum inhibitory concentration) for more than 30 days. In addition, the in vivo release behavior of the drug-eluting grafts implanted in the subcutaneous pocket of rabbits was also documented. The drug-eluting grafts developed in this work have potential applications in assisting the treatment of vascular prosthesis infection and resisting reinfection when an infected graft is to be exchanged.

No MeSH data available.


Related in: MedlinePlus