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Sustained release of vancomycin from novel biodegradable nanofiber-loaded vascular prosthetic grafts: in vitro and in vivo study.

Liu KS, Lee CH, Wang YC, Liu SJ - Int J Nanomedicine (2015)

Bottom Line: Biodegradable nanofibers were prepared by first dissolving poly(D,L)-lactide-co-glycolide and vancomycin in 1,1,1,3,3,3-hexafluoro-2-propanol.The solution was then electrospun into nanofibers onto the surface of vascular prostheses.The in vitro release rates of the pharmaceutical from the nanofiber-loaded prostheses was characterized using an elution method and a high-performance liquid chromatography assay.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan.

ABSTRACT
This study describes novel biodegradable, drug-eluting nanofiber-loaded vascular prosthetic grafts that provide local and sustained delivery of vancomycin to surrounding tissues. Biodegradable nanofibers were prepared by first dissolving poly(D,L)-lactide-co-glycolide and vancomycin in 1,1,1,3,3,3-hexafluoro-2-propanol. The solution was then electrospun into nanofibers onto the surface of vascular prostheses. The in vitro release rates of the pharmaceutical from the nanofiber-loaded prostheses was characterized using an elution method and a high-performance liquid chromatography assay. Experimental results indicated that the drug-eluting prosthetic grafts released high concentrations of vancomycin in vitro (well above the minimum inhibitory concentration) for more than 30 days. In addition, the in vivo release behavior of the drug-eluting grafts implanted in the subcutaneous pocket of rabbits was also documented. The drug-eluting grafts developed in this work have potential applications in assisting the treatment of vascular prosthesis infection and resisting reinfection when an infected graft is to be exchanged.

No MeSH data available.


Related in: MedlinePlus

Photograph of the vascular prosthetic grafts (top, loaded with nanofibers; bottom, bare graft).
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f2-ijn-10-885: Photograph of the vascular prosthetic grafts (top, loaded with nanofibers; bottom, bare graft).

Mentions: Biodegradable drug-eluting nanofibers were coated onto the external surface of prosthetic grafts (IMPRA® 6 mm ×40 cm; Bard Peripheral Vascular, Inc., Tempe, AZ, USA), using an electrospinning process. The nanofibers were prepared by first dissolving PLGA and vancomycin (240 mg and 40 mg, respectively) into 1 mL of HFIP. The solution was then delivered and electrospun by a syringe pump with a volumetric flow rate of 3.6 mL/hr to create nanofibers, onto the prosthetic grafts. The electrospinning setup of this study consisted of a syringe and needle (internal diameter of 0.42 mm), a ground electrode, a prosthetic graft mounted on a motor, a collection plate, and a high-voltage supply, as shown schematically in Figure 1. The needle was connected to the high-voltage supply, which generated positive direct current (DC) voltages and currents, up to 35 kV and 4.16mA/125W, respectively. The rotational speed of the motor was 300 rpm. The distance between the needle tip and the ground electrode was 10 cm, and the positive voltage applied to the polymer solutions was 17 kV. All electrospinning experiments were performed at room temperature. Figure 2 displays the electrospun nanofiber prosthetic grafts (top) and the bare grafts (bottom). All nanofiber-mounted prosthetic grafts were placed in a vacuum oven at 40°C for 72 hours for solvent evaporation.


Sustained release of vancomycin from novel biodegradable nanofiber-loaded vascular prosthetic grafts: in vitro and in vivo study.

Liu KS, Lee CH, Wang YC, Liu SJ - Int J Nanomedicine (2015)

Photograph of the vascular prosthetic grafts (top, loaded with nanofibers; bottom, bare graft).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321605&req=5

f2-ijn-10-885: Photograph of the vascular prosthetic grafts (top, loaded with nanofibers; bottom, bare graft).
Mentions: Biodegradable drug-eluting nanofibers were coated onto the external surface of prosthetic grafts (IMPRA® 6 mm ×40 cm; Bard Peripheral Vascular, Inc., Tempe, AZ, USA), using an electrospinning process. The nanofibers were prepared by first dissolving PLGA and vancomycin (240 mg and 40 mg, respectively) into 1 mL of HFIP. The solution was then delivered and electrospun by a syringe pump with a volumetric flow rate of 3.6 mL/hr to create nanofibers, onto the prosthetic grafts. The electrospinning setup of this study consisted of a syringe and needle (internal diameter of 0.42 mm), a ground electrode, a prosthetic graft mounted on a motor, a collection plate, and a high-voltage supply, as shown schematically in Figure 1. The needle was connected to the high-voltage supply, which generated positive direct current (DC) voltages and currents, up to 35 kV and 4.16mA/125W, respectively. The rotational speed of the motor was 300 rpm. The distance between the needle tip and the ground electrode was 10 cm, and the positive voltage applied to the polymer solutions was 17 kV. All electrospinning experiments were performed at room temperature. Figure 2 displays the electrospun nanofiber prosthetic grafts (top) and the bare grafts (bottom). All nanofiber-mounted prosthetic grafts were placed in a vacuum oven at 40°C for 72 hours for solvent evaporation.

Bottom Line: Biodegradable nanofibers were prepared by first dissolving poly(D,L)-lactide-co-glycolide and vancomycin in 1,1,1,3,3,3-hexafluoro-2-propanol.The solution was then electrospun into nanofibers onto the surface of vascular prostheses.The in vitro release rates of the pharmaceutical from the nanofiber-loaded prostheses was characterized using an elution method and a high-performance liquid chromatography assay.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan.

ABSTRACT
This study describes novel biodegradable, drug-eluting nanofiber-loaded vascular prosthetic grafts that provide local and sustained delivery of vancomycin to surrounding tissues. Biodegradable nanofibers were prepared by first dissolving poly(D,L)-lactide-co-glycolide and vancomycin in 1,1,1,3,3,3-hexafluoro-2-propanol. The solution was then electrospun into nanofibers onto the surface of vascular prostheses. The in vitro release rates of the pharmaceutical from the nanofiber-loaded prostheses was characterized using an elution method and a high-performance liquid chromatography assay. Experimental results indicated that the drug-eluting prosthetic grafts released high concentrations of vancomycin in vitro (well above the minimum inhibitory concentration) for more than 30 days. In addition, the in vivo release behavior of the drug-eluting grafts implanted in the subcutaneous pocket of rabbits was also documented. The drug-eluting grafts developed in this work have potential applications in assisting the treatment of vascular prosthesis infection and resisting reinfection when an infected graft is to be exchanged.

No MeSH data available.


Related in: MedlinePlus