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Nanocomplexation of thrombin with cationic amylose derivative for improved stability and hemostatic efficacy.

Zhuang B, Li Z, Pang J, Li W, Huang P, Wang J, Zhou Y, Lin Q, Zhou Q, Ye X, Ye H, Liu Y, Zhang LM, Chen R - Int J Nanomedicine (2015)

Bottom Line: In this work, a nanocomplexation strategy was developed for modifying the stability and hemostatic efficacy of thrombin, in which a water-soluble cationic amylose derivative containing poly(l-lysine) dendrons was prepared by a click reaction and then used to complex thrombin in an aqueous system.For resultant thrombin nanocomplexes, their morphology and particle size distribution were investigated.Via a rat hepatic hemorrhage model and a rat iliac artery hemorrhage model, these thrombin nanocomplexes were confirmed to have good tissue biocompatibility and in vivo hemostatic effectiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
As a topical hemostatic agent, thrombin has wide application for many surgical treatments. However, native thrombin always suffers from its physical and chemical instabilities. In this work, a nanocomplexation strategy was developed for modifying the stability and hemostatic efficacy of thrombin, in which a water-soluble cationic amylose derivative containing poly(l-lysine) dendrons was prepared by a click reaction and then used to complex thrombin in an aqueous system. For resultant thrombin nanocomplexes, their morphology and particle size distribution were investigated. Their stabilities were studied in terms of activity retention percentages under different storage time, pH values, and illumination time. In addition, their ability to achieve in vitro fibrinogen and blood coagulation were evaluated. Via a rat hepatic hemorrhage model and a rat iliac artery hemorrhage model, these thrombin nanocomplexes were confirmed to have good tissue biocompatibility and in vivo hemostatic effectiveness.

No MeSH data available.


Related in: MedlinePlus

(A) Photographs for the fibrinogen coagulation test in the case of the thrombin nanocomplexes. (B) Photographs for the fibrinogen coagulation test in the case of native thrombin. (C) The fibrinogen coagulation time of native thrombin and the thrombin nanocomplexes. (D) Photographs for the blood coagulation test in the case of the thrombin nanocomplexes. (E) Photographs for the blood coagulation test in the case of native thrombin. (F) The clotting time of native thrombin and the thrombin nanocomplexes.
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f5-ijn-10-939: (A) Photographs for the fibrinogen coagulation test in the case of the thrombin nanocomplexes. (B) Photographs for the fibrinogen coagulation test in the case of native thrombin. (C) The fibrinogen coagulation time of native thrombin and the thrombin nanocomplexes. (D) Photographs for the blood coagulation test in the case of the thrombin nanocomplexes. (E) Photographs for the blood coagulation test in the case of native thrombin. (F) The clotting time of native thrombin and the thrombin nanocomplexes.

Mentions: Further investigation dealt with the in vitro hemostatic efficacy of thrombin nanocomplexes by fibrinogen and blood coagulation tests. For a comparison study, native thrombin was also investigated in the same way. As shown in Figure 5, the thrombin nanocomplexes could rapidly induce the fibrinogen and blood coagulations, similar to native thrombin. There are no obvious differences between the fibrinogen coagulation/clotting time of thrombin nanocomplexes and the fibrinogen coagulation/clotting time of native thrombin. The thrombin nanocomplexes still have a strong fibrinogen and blood coagulation ability, similar to native thrombin. These results demonstrate that the nanocomplexation of thrombin with the CAD did not affect significantly the biofunctionality of thrombin.


Nanocomplexation of thrombin with cationic amylose derivative for improved stability and hemostatic efficacy.

Zhuang B, Li Z, Pang J, Li W, Huang P, Wang J, Zhou Y, Lin Q, Zhou Q, Ye X, Ye H, Liu Y, Zhang LM, Chen R - Int J Nanomedicine (2015)

(A) Photographs for the fibrinogen coagulation test in the case of the thrombin nanocomplexes. (B) Photographs for the fibrinogen coagulation test in the case of native thrombin. (C) The fibrinogen coagulation time of native thrombin and the thrombin nanocomplexes. (D) Photographs for the blood coagulation test in the case of the thrombin nanocomplexes. (E) Photographs for the blood coagulation test in the case of native thrombin. (F) The clotting time of native thrombin and the thrombin nanocomplexes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321601&req=5

f5-ijn-10-939: (A) Photographs for the fibrinogen coagulation test in the case of the thrombin nanocomplexes. (B) Photographs for the fibrinogen coagulation test in the case of native thrombin. (C) The fibrinogen coagulation time of native thrombin and the thrombin nanocomplexes. (D) Photographs for the blood coagulation test in the case of the thrombin nanocomplexes. (E) Photographs for the blood coagulation test in the case of native thrombin. (F) The clotting time of native thrombin and the thrombin nanocomplexes.
Mentions: Further investigation dealt with the in vitro hemostatic efficacy of thrombin nanocomplexes by fibrinogen and blood coagulation tests. For a comparison study, native thrombin was also investigated in the same way. As shown in Figure 5, the thrombin nanocomplexes could rapidly induce the fibrinogen and blood coagulations, similar to native thrombin. There are no obvious differences between the fibrinogen coagulation/clotting time of thrombin nanocomplexes and the fibrinogen coagulation/clotting time of native thrombin. The thrombin nanocomplexes still have a strong fibrinogen and blood coagulation ability, similar to native thrombin. These results demonstrate that the nanocomplexation of thrombin with the CAD did not affect significantly the biofunctionality of thrombin.

Bottom Line: In this work, a nanocomplexation strategy was developed for modifying the stability and hemostatic efficacy of thrombin, in which a water-soluble cationic amylose derivative containing poly(l-lysine) dendrons was prepared by a click reaction and then used to complex thrombin in an aqueous system.For resultant thrombin nanocomplexes, their morphology and particle size distribution were investigated.Via a rat hepatic hemorrhage model and a rat iliac artery hemorrhage model, these thrombin nanocomplexes were confirmed to have good tissue biocompatibility and in vivo hemostatic effectiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
As a topical hemostatic agent, thrombin has wide application for many surgical treatments. However, native thrombin always suffers from its physical and chemical instabilities. In this work, a nanocomplexation strategy was developed for modifying the stability and hemostatic efficacy of thrombin, in which a water-soluble cationic amylose derivative containing poly(l-lysine) dendrons was prepared by a click reaction and then used to complex thrombin in an aqueous system. For resultant thrombin nanocomplexes, their morphology and particle size distribution were investigated. Their stabilities were studied in terms of activity retention percentages under different storage time, pH values, and illumination time. In addition, their ability to achieve in vitro fibrinogen and blood coagulation were evaluated. Via a rat hepatic hemorrhage model and a rat iliac artery hemorrhage model, these thrombin nanocomplexes were confirmed to have good tissue biocompatibility and in vivo hemostatic effectiveness.

No MeSH data available.


Related in: MedlinePlus