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Nanocomplexation of thrombin with cationic amylose derivative for improved stability and hemostatic efficacy.

Zhuang B, Li Z, Pang J, Li W, Huang P, Wang J, Zhou Y, Lin Q, Zhou Q, Ye X, Ye H, Liu Y, Zhang LM, Chen R - Int J Nanomedicine (2015)

Bottom Line: In this work, a nanocomplexation strategy was developed for modifying the stability and hemostatic efficacy of thrombin, in which a water-soluble cationic amylose derivative containing poly(l-lysine) dendrons was prepared by a click reaction and then used to complex thrombin in an aqueous system.For resultant thrombin nanocomplexes, their morphology and particle size distribution were investigated.Via a rat hepatic hemorrhage model and a rat iliac artery hemorrhage model, these thrombin nanocomplexes were confirmed to have good tissue biocompatibility and in vivo hemostatic effectiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
As a topical hemostatic agent, thrombin has wide application for many surgical treatments. However, native thrombin always suffers from its physical and chemical instabilities. In this work, a nanocomplexation strategy was developed for modifying the stability and hemostatic efficacy of thrombin, in which a water-soluble cationic amylose derivative containing poly(l-lysine) dendrons was prepared by a click reaction and then used to complex thrombin in an aqueous system. For resultant thrombin nanocomplexes, their morphology and particle size distribution were investigated. Their stabilities were studied in terms of activity retention percentages under different storage time, pH values, and illumination time. In addition, their ability to achieve in vitro fibrinogen and blood coagulation were evaluated. Via a rat hepatic hemorrhage model and a rat iliac artery hemorrhage model, these thrombin nanocomplexes were confirmed to have good tissue biocompatibility and in vivo hemostatic effectiveness.

No MeSH data available.


Related in: MedlinePlus

Fourier transform infrared spectra of azidized amylose, propargyl focal point poly(l-lysine) dendron of the third generation and their click conjugate (CAD III).
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f2-ijn-10-939: Fourier transform infrared spectra of azidized amylose, propargyl focal point poly(l-lysine) dendron of the third generation and their click conjugate (CAD III).

Mentions: The water-soluble CAD containing poly(l-lysine) dendrons was prepared for the first time by a click conjugation reaction between azidized amylose (Amy-N3) and propargyl focal point poly(l-lysine) dendron of third-generation (PLLD-G3), as shown in Figure 1. To confirm the preparation of CAD, FTIR analyses were performed. Figure 2 gives the FTIR spectra of Amy-N3, PLLD-G3, and CAD. As seen, the characteristic absorption bands of Amy-N3 appeared at 3,460 cm−1 (νO-H, pyranose), 2,106 cm−1 (azido groups), and 1,022 cm−1 (νC-O, pyranose). The characteristic absorption bands of PLLD-G3 appeared at 2,917 cm−1, 2,842 cm−1 (νC-H), 1,604 cm−1 (νC=O), and 1,396 cm−1 (νCO-NH). After the click conjugation, the spectrum of CAD did not show the characteristic absorption bands of the azido group (2,106 cm−1) but did exhibit the main characteristic bands of Amy-N3 and PLLD-G3. These results indicate the success of the click conjugation between Amy-N3 and PLLD-G3. On the basis of elemental analysis, the degree of substitution of PLLD-G3 on the amylose, which is defined as the number of PLLD-G3 per 100 anhydroglucose units of amylose, was determined to be 9.35.


Nanocomplexation of thrombin with cationic amylose derivative for improved stability and hemostatic efficacy.

Zhuang B, Li Z, Pang J, Li W, Huang P, Wang J, Zhou Y, Lin Q, Zhou Q, Ye X, Ye H, Liu Y, Zhang LM, Chen R - Int J Nanomedicine (2015)

Fourier transform infrared spectra of azidized amylose, propargyl focal point poly(l-lysine) dendron of the third generation and their click conjugate (CAD III).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321601&req=5

f2-ijn-10-939: Fourier transform infrared spectra of azidized amylose, propargyl focal point poly(l-lysine) dendron of the third generation and their click conjugate (CAD III).
Mentions: The water-soluble CAD containing poly(l-lysine) dendrons was prepared for the first time by a click conjugation reaction between azidized amylose (Amy-N3) and propargyl focal point poly(l-lysine) dendron of third-generation (PLLD-G3), as shown in Figure 1. To confirm the preparation of CAD, FTIR analyses were performed. Figure 2 gives the FTIR spectra of Amy-N3, PLLD-G3, and CAD. As seen, the characteristic absorption bands of Amy-N3 appeared at 3,460 cm−1 (νO-H, pyranose), 2,106 cm−1 (azido groups), and 1,022 cm−1 (νC-O, pyranose). The characteristic absorption bands of PLLD-G3 appeared at 2,917 cm−1, 2,842 cm−1 (νC-H), 1,604 cm−1 (νC=O), and 1,396 cm−1 (νCO-NH). After the click conjugation, the spectrum of CAD did not show the characteristic absorption bands of the azido group (2,106 cm−1) but did exhibit the main characteristic bands of Amy-N3 and PLLD-G3. These results indicate the success of the click conjugation between Amy-N3 and PLLD-G3. On the basis of elemental analysis, the degree of substitution of PLLD-G3 on the amylose, which is defined as the number of PLLD-G3 per 100 anhydroglucose units of amylose, was determined to be 9.35.

Bottom Line: In this work, a nanocomplexation strategy was developed for modifying the stability and hemostatic efficacy of thrombin, in which a water-soluble cationic amylose derivative containing poly(l-lysine) dendrons was prepared by a click reaction and then used to complex thrombin in an aqueous system.For resultant thrombin nanocomplexes, their morphology and particle size distribution were investigated.Via a rat hepatic hemorrhage model and a rat iliac artery hemorrhage model, these thrombin nanocomplexes were confirmed to have good tissue biocompatibility and in vivo hemostatic effectiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepato-Pancreato-Billiary Surgery, Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
As a topical hemostatic agent, thrombin has wide application for many surgical treatments. However, native thrombin always suffers from its physical and chemical instabilities. In this work, a nanocomplexation strategy was developed for modifying the stability and hemostatic efficacy of thrombin, in which a water-soluble cationic amylose derivative containing poly(l-lysine) dendrons was prepared by a click reaction and then used to complex thrombin in an aqueous system. For resultant thrombin nanocomplexes, their morphology and particle size distribution were investigated. Their stabilities were studied in terms of activity retention percentages under different storage time, pH values, and illumination time. In addition, their ability to achieve in vitro fibrinogen and blood coagulation were evaluated. Via a rat hepatic hemorrhage model and a rat iliac artery hemorrhage model, these thrombin nanocomplexes were confirmed to have good tissue biocompatibility and in vivo hemostatic effectiveness.

No MeSH data available.


Related in: MedlinePlus