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Mécano-Stimulation™ of the skin improves sagging score and induces beneficial functional modification of the fibroblasts: clinical, biological, and histological evaluations.

Humbert P, Fanian F, Lihoreau T, Jeudy A, Elkhyat A, Robin S, Courderot-Masuyer C, Tauzin H, Lafforgue C, Haftek M - Clin Interv Aging (2015)

Bottom Line: A significant improvement of different clinical signs associated with skin aging and the satisfaction of the subjects were observed, correlated with an improvement of the sagging cheek.Mécano-Stimulation is a noninvasive and safe technique delivered by flaps microbeats at various frequencies, which can significantly improve the skin trophicity.Results observed with objective measurements, ie, in vitro assessments and electron microscopy, confirm the firming and restructuring effect clinically observed.

View Article: PubMed Central - PubMed

Affiliation: Research and Studies Center on the Integument (CERT), Department of Dermatology, Clinical Investigation Center (CIC 1431), Besançon University Hospital, Besançon, France ; INSERM UMR1098, FED4234 IBCT, University of Franche-Comté, Besançon, France.

ABSTRACT

Background: Loss of mechanical tension appears to be the major factor underlying decreased collagen synthesis in aged skin. Numerous in vitro studies have shown the impact of mechanical forces on fibroblasts through mechanotransduction, which consists of the conversion of mechanical signals to biochemical responses. Such responses are characterized by the modulation of gene expression coding not only for extracellular matrix components (collagens, elastin, etc.) but also for degradation enzymes (matrix metalloproteinases [MMPs]) and their inhibitors (tissue inhibitors of metalloproteinases [TIMPs]). A new device providing a mechanical stimulation of the cutaneous and subcutaneous tissue has been used in a simple, blinded, controlled, and randomized study.

Materials and methods: Thirty subjects (aged between 35 years and 50 years), with clinical signs of skin sagging, were randomly assigned to have a treatment on hemiface. After a total of 24 sessions with Mécano-Stimulation™, biopsies were performed on the treated side and control area for in vitro analysis (dosage of hyaluronic acid, elastin, type I collagen, MMP9; equivalent dermis retraction; GlaSbox(®); n=10) and electron microscopy (n=10). Furthermore, before and after the treatment, clinical evaluations and self-assessment questionnaire were done.

Results: In vitro analysis showed increases in hyaluronic acid, elastin, type I collagen, and MMP9 content along with an improvement of the migratory capacity of the fibroblasts on the treated side. Electron microscopy evaluations showed a clear dermal remodeling in relation with the activation of fibroblast activity. A significant improvement of different clinical signs associated with skin aging and the satisfaction of the subjects were observed, correlated with an improvement of the sagging cheek.

Conclusion: Mécano-Stimulation is a noninvasive and safe technique delivered by flaps microbeats at various frequencies, which can significantly improve the skin trophicity. Results observed with objective measurements, ie, in vitro assessments and electron microscopy, confirm the firming and restructuring effect clinically observed.

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Related in: MedlinePlus

Clinical scoring of skin radiance: relief surface irregularity.Notes: An analogical scale (from 0 to 9) was used to score the radiance through relief parameters: among those, surface irregularity was evaluated from small (0) to large (9) before (T0) and after (T1) the treatment. The figure represents a significant improvement (decrease) in terms of time/treatment evolution observed; surface irregularity score was improved by 20.7% on treated side (blue) against 2.5% on nontreated side (red). *P<0.05.Abbreviation: T, time.
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f5-cia-10-387: Clinical scoring of skin radiance: relief surface irregularity.Notes: An analogical scale (from 0 to 9) was used to score the radiance through relief parameters: among those, surface irregularity was evaluated from small (0) to large (9) before (T0) and after (T1) the treatment. The figure represents a significant improvement (decrease) in terms of time/treatment evolution observed; surface irregularity score was improved by 20.7% on treated side (blue) against 2.5% on nontreated side (red). *P<0.05.Abbreviation: T, time.

Mentions: At baseline (T0), no significant differences were observed in surface irregularity, fine lines, wrinkles and blemishes, skin texture, under-eye dark circles, buttons/microcysts, and relief scores between the treated and the untreated hemiface. Compared to the baseline, at T1 all scores decreased on the treated hemiface (P<0.05) (except for buttons/microcysts) while no change was observed on the nontreated hemiface. A significant improvement in terms of time/treatment evolution was observed for all the evaluated parameters (Table 3 and Figure 5). An improvement was observed for treated versus nontreated side on the following parameters:


Mécano-Stimulation™ of the skin improves sagging score and induces beneficial functional modification of the fibroblasts: clinical, biological, and histological evaluations.

Humbert P, Fanian F, Lihoreau T, Jeudy A, Elkhyat A, Robin S, Courderot-Masuyer C, Tauzin H, Lafforgue C, Haftek M - Clin Interv Aging (2015)

Clinical scoring of skin radiance: relief surface irregularity.Notes: An analogical scale (from 0 to 9) was used to score the radiance through relief parameters: among those, surface irregularity was evaluated from small (0) to large (9) before (T0) and after (T1) the treatment. The figure represents a significant improvement (decrease) in terms of time/treatment evolution observed; surface irregularity score was improved by 20.7% on treated side (blue) against 2.5% on nontreated side (red). *P<0.05.Abbreviation: T, time.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321566&req=5

f5-cia-10-387: Clinical scoring of skin radiance: relief surface irregularity.Notes: An analogical scale (from 0 to 9) was used to score the radiance through relief parameters: among those, surface irregularity was evaluated from small (0) to large (9) before (T0) and after (T1) the treatment. The figure represents a significant improvement (decrease) in terms of time/treatment evolution observed; surface irregularity score was improved by 20.7% on treated side (blue) against 2.5% on nontreated side (red). *P<0.05.Abbreviation: T, time.
Mentions: At baseline (T0), no significant differences were observed in surface irregularity, fine lines, wrinkles and blemishes, skin texture, under-eye dark circles, buttons/microcysts, and relief scores between the treated and the untreated hemiface. Compared to the baseline, at T1 all scores decreased on the treated hemiface (P<0.05) (except for buttons/microcysts) while no change was observed on the nontreated hemiface. A significant improvement in terms of time/treatment evolution was observed for all the evaluated parameters (Table 3 and Figure 5). An improvement was observed for treated versus nontreated side on the following parameters:

Bottom Line: A significant improvement of different clinical signs associated with skin aging and the satisfaction of the subjects were observed, correlated with an improvement of the sagging cheek.Mécano-Stimulation is a noninvasive and safe technique delivered by flaps microbeats at various frequencies, which can significantly improve the skin trophicity.Results observed with objective measurements, ie, in vitro assessments and electron microscopy, confirm the firming and restructuring effect clinically observed.

View Article: PubMed Central - PubMed

Affiliation: Research and Studies Center on the Integument (CERT), Department of Dermatology, Clinical Investigation Center (CIC 1431), Besançon University Hospital, Besançon, France ; INSERM UMR1098, FED4234 IBCT, University of Franche-Comté, Besançon, France.

ABSTRACT

Background: Loss of mechanical tension appears to be the major factor underlying decreased collagen synthesis in aged skin. Numerous in vitro studies have shown the impact of mechanical forces on fibroblasts through mechanotransduction, which consists of the conversion of mechanical signals to biochemical responses. Such responses are characterized by the modulation of gene expression coding not only for extracellular matrix components (collagens, elastin, etc.) but also for degradation enzymes (matrix metalloproteinases [MMPs]) and their inhibitors (tissue inhibitors of metalloproteinases [TIMPs]). A new device providing a mechanical stimulation of the cutaneous and subcutaneous tissue has been used in a simple, blinded, controlled, and randomized study.

Materials and methods: Thirty subjects (aged between 35 years and 50 years), with clinical signs of skin sagging, were randomly assigned to have a treatment on hemiface. After a total of 24 sessions with Mécano-Stimulation™, biopsies were performed on the treated side and control area for in vitro analysis (dosage of hyaluronic acid, elastin, type I collagen, MMP9; equivalent dermis retraction; GlaSbox(®); n=10) and electron microscopy (n=10). Furthermore, before and after the treatment, clinical evaluations and self-assessment questionnaire were done.

Results: In vitro analysis showed increases in hyaluronic acid, elastin, type I collagen, and MMP9 content along with an improvement of the migratory capacity of the fibroblasts on the treated side. Electron microscopy evaluations showed a clear dermal remodeling in relation with the activation of fibroblast activity. A significant improvement of different clinical signs associated with skin aging and the satisfaction of the subjects were observed, correlated with an improvement of the sagging cheek.

Conclusion: Mécano-Stimulation is a noninvasive and safe technique delivered by flaps microbeats at various frequencies, which can significantly improve the skin trophicity. Results observed with objective measurements, ie, in vitro assessments and electron microscopy, confirm the firming and restructuring effect clinically observed.

Show MeSH
Related in: MedlinePlus