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TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets.

Xue GP, Drenth J, McIntyre CL - J. Exp. Bot. (2014)

Bottom Line: Transgenic wheat plants overexpressing TaHsfA6f showed improved thermotolerance.Promoter truncation and mutagenesis analyses identified TaHsfA6f-binding elements that were responsible for transactivation of TaHSP90.1-A1 and TaGAAP by TaHsfA6f.These data suggest that TaHsfA6f is a transcriptional activator that directly regulates TaHSP, TaGAAP, and TaRof1 genes in wheat and its gene regulatory network has a positive impact on thermotolerance.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Plant Industry, 306 Carmody Road, St Lucia, Qld 4067, Australia gang-ping.xue@csiro.au.

No MeSH data available.


Related in: MedlinePlus

Relative mRNA abundance of TaHsfA6f in wheat organs and its expression response to heat stress. Values are means+SD of three biological replicates and are expressed as apparent expression levels relative to a house-keeping gene TaRP15. (A) Expression of TaHsfA6f in various organs of wheat grown under normal conditions. 1Mo, 1-month-old; 20–42DAA, 20–42 d after anthesis. (B) Expression response of TaHsfA6f to heat stress in one-month-old plants. Statistical significance of differences between control and heat-treated groups (36 °C for 1.5h, 5h, or 3 d with 5-h heat exposure per day) was analysed using Student’s t-test and is indicated by asterisks (*P<0.05; **P<0.01).
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Figure 1: Relative mRNA abundance of TaHsfA6f in wheat organs and its expression response to heat stress. Values are means+SD of three biological replicates and are expressed as apparent expression levels relative to a house-keeping gene TaRP15. (A) Expression of TaHsfA6f in various organs of wheat grown under normal conditions. 1Mo, 1-month-old; 20–42DAA, 20–42 d after anthesis. (B) Expression response of TaHsfA6f to heat stress in one-month-old plants. Statistical significance of differences between control and heat-treated groups (36 °C for 1.5h, 5h, or 3 d with 5-h heat exposure per day) was analysed using Student’s t-test and is indicated by asterisks (*P<0.05; **P<0.01).

Mentions: A full-length Hsf cDNA was isolated based on the EST sequence that contains the N-terminal half of the Hsf DNA-binding domain. Phylogenetic analysis based on conserved DNA-binding domain and HR region sequences showed that this cDNA encoded a Hsf protein that clustered with subclass HsfA6 members and was therefore designated as TaHsfA6f (Supplementary Fig. S2a). A full-length sequence alignment of TaHsfA6f with other TaHsfA members is shown in Supplementary Figure S2b. The TaHsfA6f transcript was constitutively expressed in various wheat organs with the highest expression in the mature flag leaf (Fig. 1A). Upon heat stress, the TaHsfA6f mRNA level was markedly up-regulated in both wheat leaves and roots (Fig. 1B). The heat up-regulation of TaHsfA6f was attenuated in response to prolonged heat treatment (Fig. 1B), in a similar pattern with those of other TaHsfA members (Xue et al., 2014).


TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets.

Xue GP, Drenth J, McIntyre CL - J. Exp. Bot. (2014)

Relative mRNA abundance of TaHsfA6f in wheat organs and its expression response to heat stress. Values are means+SD of three biological replicates and are expressed as apparent expression levels relative to a house-keeping gene TaRP15. (A) Expression of TaHsfA6f in various organs of wheat grown under normal conditions. 1Mo, 1-month-old; 20–42DAA, 20–42 d after anthesis. (B) Expression response of TaHsfA6f to heat stress in one-month-old plants. Statistical significance of differences between control and heat-treated groups (36 °C for 1.5h, 5h, or 3 d with 5-h heat exposure per day) was analysed using Student’s t-test and is indicated by asterisks (*P<0.05; **P<0.01).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321556&req=5

Figure 1: Relative mRNA abundance of TaHsfA6f in wheat organs and its expression response to heat stress. Values are means+SD of three biological replicates and are expressed as apparent expression levels relative to a house-keeping gene TaRP15. (A) Expression of TaHsfA6f in various organs of wheat grown under normal conditions. 1Mo, 1-month-old; 20–42DAA, 20–42 d after anthesis. (B) Expression response of TaHsfA6f to heat stress in one-month-old plants. Statistical significance of differences between control and heat-treated groups (36 °C for 1.5h, 5h, or 3 d with 5-h heat exposure per day) was analysed using Student’s t-test and is indicated by asterisks (*P<0.05; **P<0.01).
Mentions: A full-length Hsf cDNA was isolated based on the EST sequence that contains the N-terminal half of the Hsf DNA-binding domain. Phylogenetic analysis based on conserved DNA-binding domain and HR region sequences showed that this cDNA encoded a Hsf protein that clustered with subclass HsfA6 members and was therefore designated as TaHsfA6f (Supplementary Fig. S2a). A full-length sequence alignment of TaHsfA6f with other TaHsfA members is shown in Supplementary Figure S2b. The TaHsfA6f transcript was constitutively expressed in various wheat organs with the highest expression in the mature flag leaf (Fig. 1A). Upon heat stress, the TaHsfA6f mRNA level was markedly up-regulated in both wheat leaves and roots (Fig. 1B). The heat up-regulation of TaHsfA6f was attenuated in response to prolonged heat treatment (Fig. 1B), in a similar pattern with those of other TaHsfA members (Xue et al., 2014).

Bottom Line: Transgenic wheat plants overexpressing TaHsfA6f showed improved thermotolerance.Promoter truncation and mutagenesis analyses identified TaHsfA6f-binding elements that were responsible for transactivation of TaHSP90.1-A1 and TaGAAP by TaHsfA6f.These data suggest that TaHsfA6f is a transcriptional activator that directly regulates TaHSP, TaGAAP, and TaRof1 genes in wheat and its gene regulatory network has a positive impact on thermotolerance.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Plant Industry, 306 Carmody Road, St Lucia, Qld 4067, Australia gang-ping.xue@csiro.au.

No MeSH data available.


Related in: MedlinePlus