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Differential inhibition of Arabidopsis superoxide dismutases by peroxynitrite-mediated tyrosine nitration.

Holzmeister C, Gaupels F, Geerlof A, Sarioglu H, Sattler M, Durner J, Lindermayr C - J. Exp. Bot. (2014)

Bottom Line: Here, we investigated the in vitro effects of nitric oxide derivatives on the seven SOD isoforms of Arabidopsis thaliana.S-nitrosoglutathione, which causes S-nitrosylation of cysteine residues, did not influence SOD activities.The corresponding Tyr34 of human manganese SOD is also nitrated, suggesting that this might be an evolutionarily conserved mechanism for regulation of manganese SODs.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical Plant Pathology, Helmholtz Zentrum München-German Research Center for Environmental Health, 85764 München/Neuherberg, Germany.

No MeSH data available.


Enzyme activities of purified, recombinant SODs. Shown is the inhibition of formazan formation by MSD1 (A), FSDs (B), and CSDs (C). Formazan formation with heat-inactivated protein extracts was set to 100%. To distinguish between the different SOD types specific inhibitors (H2O2 for FSDs and NaCN for CSDs) were used. MSD1 is insensitive to both inhibitors.
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Figure 2: Enzyme activities of purified, recombinant SODs. Shown is the inhibition of formazan formation by MSD1 (A), FSDs (B), and CSDs (C). Formazan formation with heat-inactivated protein extracts was set to 100%. To distinguish between the different SOD types specific inhibitors (H2O2 for FSDs and NaCN for CSDs) were used. MSD1 is insensitive to both inhibitors.

Mentions: The activity of the purified, recombinant SODs was determined using the nitroblue tetrazolium (NBT)–formazan method (Fig. 2). In this assay, O2– ions are generated from the conversion of xanthine and O2 to uric acid and H2O2 by xanthine oxidase. The O2– anion then converts a NBT into a formazan dye. Addition of SOD to this reaction reduces O2– ion levels, thereby lowering the rate of formazan dye formation. SOD activity is monitored at a wavelength of 570nm and determined as the percent inhibition of the rate of formazan dye formation. The different types of SODs were verified using specific inhibitors (H2O2 for FSDs and NaCN for CSDs). MSD1 is insensitive to both inhibitors (Fig. 2).


Differential inhibition of Arabidopsis superoxide dismutases by peroxynitrite-mediated tyrosine nitration.

Holzmeister C, Gaupels F, Geerlof A, Sarioglu H, Sattler M, Durner J, Lindermayr C - J. Exp. Bot. (2014)

Enzyme activities of purified, recombinant SODs. Shown is the inhibition of formazan formation by MSD1 (A), FSDs (B), and CSDs (C). Formazan formation with heat-inactivated protein extracts was set to 100%. To distinguish between the different SOD types specific inhibitors (H2O2 for FSDs and NaCN for CSDs) were used. MSD1 is insensitive to both inhibitors.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321555&req=5

Figure 2: Enzyme activities of purified, recombinant SODs. Shown is the inhibition of formazan formation by MSD1 (A), FSDs (B), and CSDs (C). Formazan formation with heat-inactivated protein extracts was set to 100%. To distinguish between the different SOD types specific inhibitors (H2O2 for FSDs and NaCN for CSDs) were used. MSD1 is insensitive to both inhibitors.
Mentions: The activity of the purified, recombinant SODs was determined using the nitroblue tetrazolium (NBT)–formazan method (Fig. 2). In this assay, O2– ions are generated from the conversion of xanthine and O2 to uric acid and H2O2 by xanthine oxidase. The O2– anion then converts a NBT into a formazan dye. Addition of SOD to this reaction reduces O2– ion levels, thereby lowering the rate of formazan dye formation. SOD activity is monitored at a wavelength of 570nm and determined as the percent inhibition of the rate of formazan dye formation. The different types of SODs were verified using specific inhibitors (H2O2 for FSDs and NaCN for CSDs). MSD1 is insensitive to both inhibitors (Fig. 2).

Bottom Line: Here, we investigated the in vitro effects of nitric oxide derivatives on the seven SOD isoforms of Arabidopsis thaliana.S-nitrosoglutathione, which causes S-nitrosylation of cysteine residues, did not influence SOD activities.The corresponding Tyr34 of human manganese SOD is also nitrated, suggesting that this might be an evolutionarily conserved mechanism for regulation of manganese SODs.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemical Plant Pathology, Helmholtz Zentrum München-German Research Center for Environmental Health, 85764 München/Neuherberg, Germany.

No MeSH data available.