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Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice.

Wang Z, Wang Y, Hong X, Hu D, Liu C, Yang J, Li Y, Huang Y, Feng Y, Gong H, Li Y, Fang G, Tang H, Li Y - J. Exp. Bot. (2014)

Bottom Line: The SPL29 gene was identified by map-based cloning, and SPL29 was confirmed as UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) by enzymatic analysis.ROS and plant hormones probably play important roles in early leaf senescence and defence responses in the spl29 mutant.Based on these findings, it is suggested that UAP1 is involved in regulating leaf senescence and defence responses in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.

No MeSH data available.


Related in: MedlinePlus

Identification of leaf senescence in spl29 at the molecular level by qRT-PCR. (A, B) Relative expression of senescence-associated transcription factors. (C, D) Relative expression of SAGs. (E, F) Relative expression of photosynthesis-related genes. Wild-type and spl29 leaf samples were analysed at the seedling and tillering stages; the expression level of each gene in the wild type was normalised to 1. Data represent the mean ± SD of three biological replicates (Student’s t-test: **, P < 0.005; ***, P < 0.0005).
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Figure 7: Identification of leaf senescence in spl29 at the molecular level by qRT-PCR. (A, B) Relative expression of senescence-associated transcription factors. (C, D) Relative expression of SAGs. (E, F) Relative expression of photosynthesis-related genes. Wild-type and spl29 leaf samples were analysed at the seedling and tillering stages; the expression level of each gene in the wild type was normalised to 1. Data represent the mean ± SD of three biological replicates (Student’s t-test: **, P < 0.005; ***, P < 0.0005).

Mentions: Leaf senescence is a complex process controlled by a large number of different genes. Many genes that encode transcription factors are upregulated to induce leaf senescence (Zhou et al., 2013). To further confirm that senescence occurred in the spl29 plants, gene expression analysis of three senescence-associated transcription factors (OsWRKY23, OsWRKY72, and OsNAC2) was performed by qRT-PCR. At the seedling stage, transcripts of OsWRKY23, OsWRKY72, and OsNAC2 in spl29 leaves increased 40.7-, 8.5-, and 2.1-fold, respectively, in comparison to those of wild-type leaves (Fig. 7A); at the tillering stage their levels in spl29 leaves were increased 110.0-, 12.0-, and 1.5-fold, respectively, compared to the wild type (Fig. 7B). The expression levels of the senescence-associated transcription factors investigated here were all upregulated, according with the early leaf senescence of the spl29 mutant.


Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice.

Wang Z, Wang Y, Hong X, Hu D, Liu C, Yang J, Li Y, Huang Y, Feng Y, Gong H, Li Y, Fang G, Tang H, Li Y - J. Exp. Bot. (2014)

Identification of leaf senescence in spl29 at the molecular level by qRT-PCR. (A, B) Relative expression of senescence-associated transcription factors. (C, D) Relative expression of SAGs. (E, F) Relative expression of photosynthesis-related genes. Wild-type and spl29 leaf samples were analysed at the seedling and tillering stages; the expression level of each gene in the wild type was normalised to 1. Data represent the mean ± SD of three biological replicates (Student’s t-test: **, P < 0.005; ***, P < 0.0005).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321554&req=5

Figure 7: Identification of leaf senescence in spl29 at the molecular level by qRT-PCR. (A, B) Relative expression of senescence-associated transcription factors. (C, D) Relative expression of SAGs. (E, F) Relative expression of photosynthesis-related genes. Wild-type and spl29 leaf samples were analysed at the seedling and tillering stages; the expression level of each gene in the wild type was normalised to 1. Data represent the mean ± SD of three biological replicates (Student’s t-test: **, P < 0.005; ***, P < 0.0005).
Mentions: Leaf senescence is a complex process controlled by a large number of different genes. Many genes that encode transcription factors are upregulated to induce leaf senescence (Zhou et al., 2013). To further confirm that senescence occurred in the spl29 plants, gene expression analysis of three senescence-associated transcription factors (OsWRKY23, OsWRKY72, and OsNAC2) was performed by qRT-PCR. At the seedling stage, transcripts of OsWRKY23, OsWRKY72, and OsNAC2 in spl29 leaves increased 40.7-, 8.5-, and 2.1-fold, respectively, in comparison to those of wild-type leaves (Fig. 7A); at the tillering stage their levels in spl29 leaves were increased 110.0-, 12.0-, and 1.5-fold, respectively, compared to the wild type (Fig. 7B). The expression levels of the senescence-associated transcription factors investigated here were all upregulated, according with the early leaf senescence of the spl29 mutant.

Bottom Line: The SPL29 gene was identified by map-based cloning, and SPL29 was confirmed as UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) by enzymatic analysis.ROS and plant hormones probably play important roles in early leaf senescence and defence responses in the spl29 mutant.Based on these findings, it is suggested that UAP1 is involved in regulating leaf senescence and defence responses in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.

No MeSH data available.


Related in: MedlinePlus