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Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice.

Wang Z, Wang Y, Hong X, Hu D, Liu C, Yang J, Li Y, Huang Y, Feng Y, Gong H, Li Y, Fang G, Tang H, Li Y - J. Exp. Bot. (2014)

Bottom Line: The SPL29 gene was identified by map-based cloning, and SPL29 was confirmed as UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) by enzymatic analysis.ROS and plant hormones probably play important roles in early leaf senescence and defence responses in the spl29 mutant.Based on these findings, it is suggested that UAP1 is involved in regulating leaf senescence and defence responses in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.

No MeSH data available.


Related in: MedlinePlus

Identification of leaf senescence in spl29 at the physiological level. (A, B) Chlorophyll contents. FW, fresh weight. (C, D) Relative expression of the senescence-induced SGR gene by qRT-PCR. The expression level of SGR in the wild type was normalised to 1. In A–D, wild-type and spl29 leaf samples were analysed at the seedling and tillering stages; data represent the mean ± SD of three or four biological replicates (Student’s t-test: ***, P < 0.0005). (E) Chlorophyll a fluorescence transients. (F) Analysis of fluorescence parameters (see also Supplementary Table S4). (E–F) Each data point is the mean value of ten independent plants.
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Figure 5: Identification of leaf senescence in spl29 at the physiological level. (A, B) Chlorophyll contents. FW, fresh weight. (C, D) Relative expression of the senescence-induced SGR gene by qRT-PCR. The expression level of SGR in the wild type was normalised to 1. In A–D, wild-type and spl29 leaf samples were analysed at the seedling and tillering stages; data represent the mean ± SD of three or four biological replicates (Student’s t-test: ***, P < 0.0005). (E) Chlorophyll a fluorescence transients. (F) Analysis of fluorescence parameters (see also Supplementary Table S4). (E–F) Each data point is the mean value of ten independent plants.

Mentions: The decrease of chlorophyll content is commonly used as a physiological indicator related to plant leaf senescence (Jiao et al., 2012). At the seedling stage, chlorophyll content decreased from 1240 μg g–l FW in wild-type leaves to 861 μg g–1 FW in spl29 leaves (Fig. 5A). A similar reduction was also observed at the tillering stage, when chlorophyll contents were 1362 μg g–1 FW in the wild type and 786 μg g–1 FW in spl29 (Fig. 5B). The senescence-induced STAY GREEN (SGR) gene plays an important role in regulating chlorophyll degradation (Park et al., 2007). Analysis by qRT-PCR showed that the expression of SGR was greatly upregulated in spl29 leaves at the seedling and tillering stages (Fig. 5C–D).


Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice.

Wang Z, Wang Y, Hong X, Hu D, Liu C, Yang J, Li Y, Huang Y, Feng Y, Gong H, Li Y, Fang G, Tang H, Li Y - J. Exp. Bot. (2014)

Identification of leaf senescence in spl29 at the physiological level. (A, B) Chlorophyll contents. FW, fresh weight. (C, D) Relative expression of the senescence-induced SGR gene by qRT-PCR. The expression level of SGR in the wild type was normalised to 1. In A–D, wild-type and spl29 leaf samples were analysed at the seedling and tillering stages; data represent the mean ± SD of three or four biological replicates (Student’s t-test: ***, P < 0.0005). (E) Chlorophyll a fluorescence transients. (F) Analysis of fluorescence parameters (see also Supplementary Table S4). (E–F) Each data point is the mean value of ten independent plants.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321554&req=5

Figure 5: Identification of leaf senescence in spl29 at the physiological level. (A, B) Chlorophyll contents. FW, fresh weight. (C, D) Relative expression of the senescence-induced SGR gene by qRT-PCR. The expression level of SGR in the wild type was normalised to 1. In A–D, wild-type and spl29 leaf samples were analysed at the seedling and tillering stages; data represent the mean ± SD of three or four biological replicates (Student’s t-test: ***, P < 0.0005). (E) Chlorophyll a fluorescence transients. (F) Analysis of fluorescence parameters (see also Supplementary Table S4). (E–F) Each data point is the mean value of ten independent plants.
Mentions: The decrease of chlorophyll content is commonly used as a physiological indicator related to plant leaf senescence (Jiao et al., 2012). At the seedling stage, chlorophyll content decreased from 1240 μg g–l FW in wild-type leaves to 861 μg g–1 FW in spl29 leaves (Fig. 5A). A similar reduction was also observed at the tillering stage, when chlorophyll contents were 1362 μg g–1 FW in the wild type and 786 μg g–1 FW in spl29 (Fig. 5B). The senescence-induced STAY GREEN (SGR) gene plays an important role in regulating chlorophyll degradation (Park et al., 2007). Analysis by qRT-PCR showed that the expression of SGR was greatly upregulated in spl29 leaves at the seedling and tillering stages (Fig. 5C–D).

Bottom Line: The SPL29 gene was identified by map-based cloning, and SPL29 was confirmed as UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) by enzymatic analysis.ROS and plant hormones probably play important roles in early leaf senescence and defence responses in the spl29 mutant.Based on these findings, it is suggested that UAP1 is involved in regulating leaf senescence and defence responses in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.

No MeSH data available.


Related in: MedlinePlus