Limits...
Higher sterol content regulated by CYP51 with concomitant lower phospholipid content in membranes is a common strategy for aluminium tolerance in several plant species.

Wagatsuma T, Khan MS, Watanabe T, Maejima E, Sekimoto H, Yokota T, Nakano T, Toyomasu T, Tawaraya K, Koyama H, Uemura M, Ishikawa S, Ikka T, Ishikawa A, Kawamura T, Murakami S, Ueki N, Umetsu A, Kannari T - J. Exp. Bot. (2014)

Bottom Line: Several studies have shown that differences in lipid composition and in the lipid biosynthetic pathway affect the aluminium (Al) tolerance of plants, but little is known about the molecular mechanisms underlying these differences.Currency).This appears to be a common strategy for Al tolerance among several plant species.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Agriculture, Yamagata University, Tsuruoka 997-8555, Japan wagatuma@tds1.tr.yamagata-u.ac.jp.

No MeSH data available.


Relationship between relative transcript level of CYP51 and phospholipid (PL)/sterol (S) ratio or sterols. Total RNA was extracted from frozen 1-cm root tips of 5-day-old pea seedlings and used for real-time qRT-PCR. The relative transcript level of CYP51 was normalized to that of 18S rRNA (internal control). PL and S were extracted from 1-cm root tips of 5-day-old pea seedlings. The dotted line shows the relationship between S and relative transcript levels of CYP51. The solid line shows the relationship between relative transcript level of CYP51 and PL/S ratio. HC, cv. Harunoka (control); HA, cv. Harunoka (Al treatment); lC, lh mutant (control); lA, lh mutant (Al treatment). Values are means of two independent replicates ± standard error.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4321553&req=5

Figure 4: Relationship between relative transcript level of CYP51 and phospholipid (PL)/sterol (S) ratio or sterols. Total RNA was extracted from frozen 1-cm root tips of 5-day-old pea seedlings and used for real-time qRT-PCR. The relative transcript level of CYP51 was normalized to that of 18S rRNA (internal control). PL and S were extracted from 1-cm root tips of 5-day-old pea seedlings. The dotted line shows the relationship between S and relative transcript levels of CYP51. The solid line shows the relationship between relative transcript level of CYP51 and PL/S ratio. HC, cv. Harunoka (control); HA, cv. Harunoka (Al treatment); lC, lh mutant (control); lA, lh mutant (Al treatment). Values are means of two independent replicates ± standard error.

Mentions: Next, the lipid composition and expression levels of CYP51 were evaluated in the Al-sensitive and Al-tolerant pea genotypes. The CYP51 homologue in pea was cloned by degenerate PCR using primers designed from CYP51 sequences in other legumes [barrel clover (Medicago truncatula L.) and soybean (Glycine max Merr.)]. A full-length cDNA, which showed high homology to orthologues in other legumes, encoded 489 amino acids. The nucleotide sequence of PsCYP51 has been deposited in the GenBank/EMBL database [Accession number AB633330]. The PsCYP51 transcript levels in the cultivar and the mutant were determined by qRT-PCR with specific primers. The highest concentration of sterols in the root-tip portion was in the Al-tolerant cv. Harunoka, while the highest concentration of phospholipids was in the most Al-sensitive line, the lh mutant (Fig. 3). In general, Al treatment decreased the sterol content (Fig. 3, open bars) and increased the phospholipid content (Fig. 3, closed bars). The highest ratio of phospholipids to sterols was in the most Al-sensitive line, the lh mutant. The relative level of CYP51 mRNA in the root tip of pea was positively correlated with sterol content (R2 = 0.915, P < 0.05), but negatively correlated with the ratio of phospholipids to sterols (R2 = 0.907, P < 0.05) (Fig. 4). These results suggest that the sterol/phospholipid ratio, which is regulated by the biosynthesis of sterols, plays a role in determining Al tolerance.


Higher sterol content regulated by CYP51 with concomitant lower phospholipid content in membranes is a common strategy for aluminium tolerance in several plant species.

Wagatsuma T, Khan MS, Watanabe T, Maejima E, Sekimoto H, Yokota T, Nakano T, Toyomasu T, Tawaraya K, Koyama H, Uemura M, Ishikawa S, Ikka T, Ishikawa A, Kawamura T, Murakami S, Ueki N, Umetsu A, Kannari T - J. Exp. Bot. (2014)

Relationship between relative transcript level of CYP51 and phospholipid (PL)/sterol (S) ratio or sterols. Total RNA was extracted from frozen 1-cm root tips of 5-day-old pea seedlings and used for real-time qRT-PCR. The relative transcript level of CYP51 was normalized to that of 18S rRNA (internal control). PL and S were extracted from 1-cm root tips of 5-day-old pea seedlings. The dotted line shows the relationship between S and relative transcript levels of CYP51. The solid line shows the relationship between relative transcript level of CYP51 and PL/S ratio. HC, cv. Harunoka (control); HA, cv. Harunoka (Al treatment); lC, lh mutant (control); lA, lh mutant (Al treatment). Values are means of two independent replicates ± standard error.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321553&req=5

Figure 4: Relationship between relative transcript level of CYP51 and phospholipid (PL)/sterol (S) ratio or sterols. Total RNA was extracted from frozen 1-cm root tips of 5-day-old pea seedlings and used for real-time qRT-PCR. The relative transcript level of CYP51 was normalized to that of 18S rRNA (internal control). PL and S were extracted from 1-cm root tips of 5-day-old pea seedlings. The dotted line shows the relationship between S and relative transcript levels of CYP51. The solid line shows the relationship between relative transcript level of CYP51 and PL/S ratio. HC, cv. Harunoka (control); HA, cv. Harunoka (Al treatment); lC, lh mutant (control); lA, lh mutant (Al treatment). Values are means of two independent replicates ± standard error.
Mentions: Next, the lipid composition and expression levels of CYP51 were evaluated in the Al-sensitive and Al-tolerant pea genotypes. The CYP51 homologue in pea was cloned by degenerate PCR using primers designed from CYP51 sequences in other legumes [barrel clover (Medicago truncatula L.) and soybean (Glycine max Merr.)]. A full-length cDNA, which showed high homology to orthologues in other legumes, encoded 489 amino acids. The nucleotide sequence of PsCYP51 has been deposited in the GenBank/EMBL database [Accession number AB633330]. The PsCYP51 transcript levels in the cultivar and the mutant were determined by qRT-PCR with specific primers. The highest concentration of sterols in the root-tip portion was in the Al-tolerant cv. Harunoka, while the highest concentration of phospholipids was in the most Al-sensitive line, the lh mutant (Fig. 3). In general, Al treatment decreased the sterol content (Fig. 3, open bars) and increased the phospholipid content (Fig. 3, closed bars). The highest ratio of phospholipids to sterols was in the most Al-sensitive line, the lh mutant. The relative level of CYP51 mRNA in the root tip of pea was positively correlated with sterol content (R2 = 0.915, P < 0.05), but negatively correlated with the ratio of phospholipids to sterols (R2 = 0.907, P < 0.05) (Fig. 4). These results suggest that the sterol/phospholipid ratio, which is regulated by the biosynthesis of sterols, plays a role in determining Al tolerance.

Bottom Line: Several studies have shown that differences in lipid composition and in the lipid biosynthetic pathway affect the aluminium (Al) tolerance of plants, but little is known about the molecular mechanisms underlying these differences.Currency).This appears to be a common strategy for Al tolerance among several plant species.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Agriculture, Yamagata University, Tsuruoka 997-8555, Japan wagatuma@tds1.tr.yamagata-u.ac.jp.

No MeSH data available.