Limits...
Semi-dwarfism and lodging tolerance in tef (Eragrostis tef) is linked to a mutation in the α-Tubulin 1 gene.

Jöst M, Esfeld K, Burian A, Cannarozzi G, Chanyalew S, Kuhlemeier C, Assefa K, Tadele Z - J. Exp. Bot. (2014)

Bottom Line: The response of kegne to microtubule-depolymerizing and -stabilizing drugs, as well as subsequent gene sequencing and segregation analysis, suggests that a defect in the α-Tubulin gene is functionally and genetically tightly linked to the kegne phenotype.In diploid species such as rice, homozygous mutations in α-Tubulin genes result in extreme dwarfism and weak stems.In the allotetraploid tef, only one homeologue is mutated, and the presence of the second intact α-Tubulin gene copy confers the agriculturally beneficial semi-dwarf and lodging-tolerant phenotype.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Sciences, University of Bern, Altenbergrain 21, CH-3013 Bern, Switzerland.

No MeSH data available.


Related in: MedlinePlus

The response of kegne to propyzamide and oryzalin. (A) The twisting phenotype of kegne was induced in 3-day-old WT plants treated with 2 μM propyzamide. Scale bar=1cm. Effect of different concentrations of oryzalin on the height of (B) WT and (C) kegne plants grown for 10 d in the light. Values with the same letters are not significantly different at the P<0.05 level after Tukey test (n=15). Error bars indicate 1 SD. The height of seedlings transferred to the medium without oryzalin and grown for an additional 6 d to recover from (D) 0.05 μM (n=15) and (E) 0.1 μM oryzalin (n=15).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4321551&req=5

Figure 3: The response of kegne to propyzamide and oryzalin. (A) The twisting phenotype of kegne was induced in 3-day-old WT plants treated with 2 μM propyzamide. Scale bar=1cm. Effect of different concentrations of oryzalin on the height of (B) WT and (C) kegne plants grown for 10 d in the light. Values with the same letters are not significantly different at the P<0.05 level after Tukey test (n=15). Error bars indicate 1 SD. The height of seedlings transferred to the medium without oryzalin and grown for an additional 6 d to recover from (D) 0.05 μM (n=15) and (E) 0.1 μM oryzalin (n=15).

Mentions: Propyzamide also converted WT plants to the helical phenotype observed in the kegne mutant (Fig. 3A). The fact that twisting was induced in WT plants treated with a microtubule-destabilizing drug strongly suggests that this phenotype of kegne is caused by defects associated with microtubules.


Semi-dwarfism and lodging tolerance in tef (Eragrostis tef) is linked to a mutation in the α-Tubulin 1 gene.

Jöst M, Esfeld K, Burian A, Cannarozzi G, Chanyalew S, Kuhlemeier C, Assefa K, Tadele Z - J. Exp. Bot. (2014)

The response of kegne to propyzamide and oryzalin. (A) The twisting phenotype of kegne was induced in 3-day-old WT plants treated with 2 μM propyzamide. Scale bar=1cm. Effect of different concentrations of oryzalin on the height of (B) WT and (C) kegne plants grown for 10 d in the light. Values with the same letters are not significantly different at the P<0.05 level after Tukey test (n=15). Error bars indicate 1 SD. The height of seedlings transferred to the medium without oryzalin and grown for an additional 6 d to recover from (D) 0.05 μM (n=15) and (E) 0.1 μM oryzalin (n=15).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321551&req=5

Figure 3: The response of kegne to propyzamide and oryzalin. (A) The twisting phenotype of kegne was induced in 3-day-old WT plants treated with 2 μM propyzamide. Scale bar=1cm. Effect of different concentrations of oryzalin on the height of (B) WT and (C) kegne plants grown for 10 d in the light. Values with the same letters are not significantly different at the P<0.05 level after Tukey test (n=15). Error bars indicate 1 SD. The height of seedlings transferred to the medium without oryzalin and grown for an additional 6 d to recover from (D) 0.05 μM (n=15) and (E) 0.1 μM oryzalin (n=15).
Mentions: Propyzamide also converted WT plants to the helical phenotype observed in the kegne mutant (Fig. 3A). The fact that twisting was induced in WT plants treated with a microtubule-destabilizing drug strongly suggests that this phenotype of kegne is caused by defects associated with microtubules.

Bottom Line: The response of kegne to microtubule-depolymerizing and -stabilizing drugs, as well as subsequent gene sequencing and segregation analysis, suggests that a defect in the α-Tubulin gene is functionally and genetically tightly linked to the kegne phenotype.In diploid species such as rice, homozygous mutations in α-Tubulin genes result in extreme dwarfism and weak stems.In the allotetraploid tef, only one homeologue is mutated, and the presence of the second intact α-Tubulin gene copy confers the agriculturally beneficial semi-dwarf and lodging-tolerant phenotype.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Sciences, University of Bern, Altenbergrain 21, CH-3013 Bern, Switzerland.

No MeSH data available.


Related in: MedlinePlus