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Semi-dwarfism and lodging tolerance in tef (Eragrostis tef) is linked to a mutation in the α-Tubulin 1 gene.

Jöst M, Esfeld K, Burian A, Cannarozzi G, Chanyalew S, Kuhlemeier C, Assefa K, Tadele Z - J. Exp. Bot. (2014)

Bottom Line: The response of kegne to microtubule-depolymerizing and -stabilizing drugs, as well as subsequent gene sequencing and segregation analysis, suggests that a defect in the α-Tubulin gene is functionally and genetically tightly linked to the kegne phenotype.In diploid species such as rice, homozygous mutations in α-Tubulin genes result in extreme dwarfism and weak stems.In the allotetraploid tef, only one homeologue is mutated, and the presence of the second intact α-Tubulin gene copy confers the agriculturally beneficial semi-dwarf and lodging-tolerant phenotype.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Sciences, University of Bern, Altenbergrain 21, CH-3013 Bern, Switzerland.

No MeSH data available.


Related in: MedlinePlus

Kegne plants twist in the right-hand direction, and are semi-dwarf and lodging tolerant. Nine-day-old shoots of (A) WT and (B) kegne, and 3-day-old coleoptiles of (C) WT and (D) kegne in a growth room. (E) The angle of tilting was significantly higher for kegne compared with the WT (P=1.83 E-76 after ANOVA, n=96, error bars indicate 1 SD). (F) The height of both genotypes from germination to harvest maturity; error bars indicate 1 SD (n=10). Two-month-old (G) WT and (H) kegne plants in the greenhouse. Scale bars in A and B=1cm, in C and D=500 μM, in G and H=10cm.
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Figure 1: Kegne plants twist in the right-hand direction, and are semi-dwarf and lodging tolerant. Nine-day-old shoots of (A) WT and (B) kegne, and 3-day-old coleoptiles of (C) WT and (D) kegne in a growth room. (E) The angle of tilting was significantly higher for kegne compared with the WT (P=1.83 E-76 after ANOVA, n=96, error bars indicate 1 SD). (F) The height of both genotypes from germination to harvest maturity; error bars indicate 1 SD (n=10). Two-month-old (G) WT and (H) kegne plants in the greenhouse. Scale bars in A and B=1cm, in C and D=500 μM, in G and H=10cm.

Mentions: A tef mutant with a helical phenotype was identified from an EMS-mutagenized M2 population of the improved tef cultivar DZ-Cr-37 (also known as Tsedey; here referred to as the WT). The mutant was named kegne, meaning ‘right hand’ in the Ethiopian Amharic language, for the unique turning of its first leaves towards the right-hand direction (Fig. 1A, B). The helical phenotype of kegne was also pronounced in dark-grown coleoptiles (Fig. 1C, D), with cells tilting off the vertical axis on average by ~12 ° compared with only 2 ° in the WT (Fig. 1E). Strikingly, the cells of kegne coleoptiles twisted in all cases in the right-hand (or clock-wise) direction, whereas those in the WT tilted off the central axis in either orientation.


Semi-dwarfism and lodging tolerance in tef (Eragrostis tef) is linked to a mutation in the α-Tubulin 1 gene.

Jöst M, Esfeld K, Burian A, Cannarozzi G, Chanyalew S, Kuhlemeier C, Assefa K, Tadele Z - J. Exp. Bot. (2014)

Kegne plants twist in the right-hand direction, and are semi-dwarf and lodging tolerant. Nine-day-old shoots of (A) WT and (B) kegne, and 3-day-old coleoptiles of (C) WT and (D) kegne in a growth room. (E) The angle of tilting was significantly higher for kegne compared with the WT (P=1.83 E-76 after ANOVA, n=96, error bars indicate 1 SD). (F) The height of both genotypes from germination to harvest maturity; error bars indicate 1 SD (n=10). Two-month-old (G) WT and (H) kegne plants in the greenhouse. Scale bars in A and B=1cm, in C and D=500 μM, in G and H=10cm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 1: Kegne plants twist in the right-hand direction, and are semi-dwarf and lodging tolerant. Nine-day-old shoots of (A) WT and (B) kegne, and 3-day-old coleoptiles of (C) WT and (D) kegne in a growth room. (E) The angle of tilting was significantly higher for kegne compared with the WT (P=1.83 E-76 after ANOVA, n=96, error bars indicate 1 SD). (F) The height of both genotypes from germination to harvest maturity; error bars indicate 1 SD (n=10). Two-month-old (G) WT and (H) kegne plants in the greenhouse. Scale bars in A and B=1cm, in C and D=500 μM, in G and H=10cm.
Mentions: A tef mutant with a helical phenotype was identified from an EMS-mutagenized M2 population of the improved tef cultivar DZ-Cr-37 (also known as Tsedey; here referred to as the WT). The mutant was named kegne, meaning ‘right hand’ in the Ethiopian Amharic language, for the unique turning of its first leaves towards the right-hand direction (Fig. 1A, B). The helical phenotype of kegne was also pronounced in dark-grown coleoptiles (Fig. 1C, D), with cells tilting off the vertical axis on average by ~12 ° compared with only 2 ° in the WT (Fig. 1E). Strikingly, the cells of kegne coleoptiles twisted in all cases in the right-hand (or clock-wise) direction, whereas those in the WT tilted off the central axis in either orientation.

Bottom Line: The response of kegne to microtubule-depolymerizing and -stabilizing drugs, as well as subsequent gene sequencing and segregation analysis, suggests that a defect in the α-Tubulin gene is functionally and genetically tightly linked to the kegne phenotype.In diploid species such as rice, homozygous mutations in α-Tubulin genes result in extreme dwarfism and weak stems.In the allotetraploid tef, only one homeologue is mutated, and the presence of the second intact α-Tubulin gene copy confers the agriculturally beneficial semi-dwarf and lodging-tolerant phenotype.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Sciences, University of Bern, Altenbergrain 21, CH-3013 Bern, Switzerland.

No MeSH data available.


Related in: MedlinePlus