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Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato.

Albacete A, Cantero-Navarro E, Großkinsky DK, Arias CL, Balibrea ME, Bru R, Fragner L, Ghanem ME, González Mde L, Hernández JA, Martínez-Andújar C, van der Graaff E, Weckwerth W, Zellnig G, Pérez-Alfocea F, Roitsch T - J. Exp. Bot. (2014)

Bottom Line: Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants.Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation.Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the senescence-delaying hormone trans-zeatin and decreases in the senescence-inducing ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in the leaves.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, CEBAS-CSIC, Campus de Espinardo, 30100 Murcia, Spain Institute of Plant Sciences, Department of Plant Physiology, University of Graz, 8010 Graz, Austria.

No MeSH data available.


Related in: MedlinePlus

Principal component analysis of those metabolites analysed in mature leaves of the WT and two CIN1 lines (CIN1-91 and CIN1-93) which showed significant differences between control (C) and drought stress (DS) conditions. Circles indicate samples that cluster together. The arrow indicates a shift of the WT compared with CIN1 plants following 9 d of drought stress.
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Figure 9: Principal component analysis of those metabolites analysed in mature leaves of the WT and two CIN1 lines (CIN1-91 and CIN1-93) which showed significant differences between control (C) and drought stress (DS) conditions. Circles indicate samples that cluster together. The arrow indicates a shift of the WT compared with CIN1 plants following 9 d of drought stress.

Mentions: To characterize further the mechanism of drought stress tolerance in CIN1 plants, metabolite profiling was performed. This identified 100 compounds, of which 22 showed significant changes due to drought stress conditions (Table 3). CIN1 plants showed specific accumulation of phosphorylated sugar intermediates glucose-6-phosphate and fructose-6-phosphate, organic acids, and phenolic compounds. Five out of six mono- and disaccharides and a sugar-alcohol were reduced in the leaves of transgenic lines under drought conditions, whereas amino acids showed a differential response (Table 3). This set of metabolites was subjected to principal component analysis (PCA). Although the CIN1 plants clustered together with the WT plants under control conditions, at the end of the drought period CIN1 plants clearly clustered separately from WT plants (Fig. 9). The fact that the stressed CIN1 plants clustered in between plants under control conditions and stressed WT plants for the principal component 1 (PC1) indicates that CIN1 plants are less affected by drought stress conditions (Fig. 8).


Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato.

Albacete A, Cantero-Navarro E, Großkinsky DK, Arias CL, Balibrea ME, Bru R, Fragner L, Ghanem ME, González Mde L, Hernández JA, Martínez-Andújar C, van der Graaff E, Weckwerth W, Zellnig G, Pérez-Alfocea F, Roitsch T - J. Exp. Bot. (2014)

Principal component analysis of those metabolites analysed in mature leaves of the WT and two CIN1 lines (CIN1-91 and CIN1-93) which showed significant differences between control (C) and drought stress (DS) conditions. Circles indicate samples that cluster together. The arrow indicates a shift of the WT compared with CIN1 plants following 9 d of drought stress.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321548&req=5

Figure 9: Principal component analysis of those metabolites analysed in mature leaves of the WT and two CIN1 lines (CIN1-91 and CIN1-93) which showed significant differences between control (C) and drought stress (DS) conditions. Circles indicate samples that cluster together. The arrow indicates a shift of the WT compared with CIN1 plants following 9 d of drought stress.
Mentions: To characterize further the mechanism of drought stress tolerance in CIN1 plants, metabolite profiling was performed. This identified 100 compounds, of which 22 showed significant changes due to drought stress conditions (Table 3). CIN1 plants showed specific accumulation of phosphorylated sugar intermediates glucose-6-phosphate and fructose-6-phosphate, organic acids, and phenolic compounds. Five out of six mono- and disaccharides and a sugar-alcohol were reduced in the leaves of transgenic lines under drought conditions, whereas amino acids showed a differential response (Table 3). This set of metabolites was subjected to principal component analysis (PCA). Although the CIN1 plants clustered together with the WT plants under control conditions, at the end of the drought period CIN1 plants clearly clustered separately from WT plants (Fig. 9). The fact that the stressed CIN1 plants clustered in between plants under control conditions and stressed WT plants for the principal component 1 (PC1) indicates that CIN1 plants are less affected by drought stress conditions (Fig. 8).

Bottom Line: Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants.Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation.Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the senescence-delaying hormone trans-zeatin and decreases in the senescence-inducing ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in the leaves.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, CEBAS-CSIC, Campus de Espinardo, 30100 Murcia, Spain Institute of Plant Sciences, Department of Plant Physiology, University of Graz, 8010 Graz, Austria.

No MeSH data available.


Related in: MedlinePlus