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Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato.

Albacete A, Cantero-Navarro E, Großkinsky DK, Arias CL, Balibrea ME, Bru R, Fragner L, Ghanem ME, González Mde L, Hernández JA, Martínez-Andújar C, van der Graaff E, Weckwerth W, Zellnig G, Pérez-Alfocea F, Roitsch T - J. Exp. Bot. (2014)

Bottom Line: Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants.Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation.Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the senescence-delaying hormone trans-zeatin and decreases in the senescence-inducing ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in the leaves.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, CEBAS-CSIC, Campus de Espinardo, 30100 Murcia, Spain Institute of Plant Sciences, Department of Plant Physiology, University of Graz, 8010 Graz, Austria.

No MeSH data available.


Related in: MedlinePlus

trans-Zeatin (t-Z; A), the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC; B), indoleacetic acid (IAA; C), and abscisic acid (ABA; D) concentrations in tomato leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress. Data are presented as means ±SE, *P<0.05, one-way ANOVA, n=3.
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Figure 8: trans-Zeatin (t-Z; A), the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC; B), indoleacetic acid (IAA; C), and abscisic acid (ABA; D) concentrations in tomato leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress. Data are presented as means ±SE, *P<0.05, one-way ANOVA, n=3.

Mentions: Leaf concentrations of the active cytokinin (CK) trans-zeatin (tZ) and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) were analysed in control and water-stressed plants at the end of the experiment. Although tZ levels were reduced under drought stress, CIN1 lines that showed a strong phenotype also presented a significant increase in tZ compared with WT plants (Fig. 8A). Drought stress resulted in a general increase in the ACC concentrations in WT and CIN1 plants, but remained significantly lower (by 25%) in the CIN1 lines, except for the aphenotypic CIN1-8 line (Fig. 8B). While auxin levels were slightly increased in the transgenic plants (Fig. 8C), no clear trend was evident for the abscisic acid (ABA) levels (Fig. 8D).


Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato.

Albacete A, Cantero-Navarro E, Großkinsky DK, Arias CL, Balibrea ME, Bru R, Fragner L, Ghanem ME, González Mde L, Hernández JA, Martínez-Andújar C, van der Graaff E, Weckwerth W, Zellnig G, Pérez-Alfocea F, Roitsch T - J. Exp. Bot. (2014)

trans-Zeatin (t-Z; A), the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC; B), indoleacetic acid (IAA; C), and abscisic acid (ABA; D) concentrations in tomato leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress. Data are presented as means ±SE, *P<0.05, one-way ANOVA, n=3.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321548&req=5

Figure 8: trans-Zeatin (t-Z; A), the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC; B), indoleacetic acid (IAA; C), and abscisic acid (ABA; D) concentrations in tomato leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress. Data are presented as means ±SE, *P<0.05, one-way ANOVA, n=3.
Mentions: Leaf concentrations of the active cytokinin (CK) trans-zeatin (tZ) and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) were analysed in control and water-stressed plants at the end of the experiment. Although tZ levels were reduced under drought stress, CIN1 lines that showed a strong phenotype also presented a significant increase in tZ compared with WT plants (Fig. 8A). Drought stress resulted in a general increase in the ACC concentrations in WT and CIN1 plants, but remained significantly lower (by 25%) in the CIN1 lines, except for the aphenotypic CIN1-8 line (Fig. 8B). While auxin levels were slightly increased in the transgenic plants (Fig. 8C), no clear trend was evident for the abscisic acid (ABA) levels (Fig. 8D).

Bottom Line: Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants.Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation.Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the senescence-delaying hormone trans-zeatin and decreases in the senescence-inducing ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in the leaves.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, CEBAS-CSIC, Campus de Espinardo, 30100 Murcia, Spain Institute of Plant Sciences, Department of Plant Physiology, University of Graz, 8010 Graz, Austria.

No MeSH data available.


Related in: MedlinePlus