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Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato.

Albacete A, Cantero-Navarro E, Großkinsky DK, Arias CL, Balibrea ME, Bru R, Fragner L, Ghanem ME, González Mde L, Hernández JA, Martínez-Andújar C, van der Graaff E, Weckwerth W, Zellnig G, Pérez-Alfocea F, Roitsch T - J. Exp. Bot. (2014)

Bottom Line: Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants.Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation.Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the senescence-delaying hormone trans-zeatin and decreases in the senescence-inducing ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in the leaves.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, CEBAS-CSIC, Campus de Espinardo, 30100 Murcia, Spain Institute of Plant Sciences, Department of Plant Physiology, University of Graz, 8010 Graz, Austria.

No MeSH data available.


Related in: MedlinePlus

Reference 2D electrophoretic pattern of mature leaf apoplast from WT plants and the CIN1-91 line growing under normal watering regimes and after 9 d of drought stress. Proteins were resolved using a linear pH gradient of 4–7 in the first dimension and 12% SDS–PAGE in the second dimension. Selected spots are indicated with arrows.
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Figure 7: Reference 2D electrophoretic pattern of mature leaf apoplast from WT plants and the CIN1-91 line growing under normal watering regimes and after 9 d of drought stress. Proteins were resolved using a linear pH gradient of 4–7 in the first dimension and 12% SDS–PAGE in the second dimension. Selected spots are indicated with arrows.

Mentions: During stress, disruption of cellular homeostasis is accompanied by the generation of reactive oxygen species (ROS), and the extent of stress-induced damage can be attenuated by the action of the cell’s antioxidant systems, including glutathione and enzymes capable of scavenging ROS. The oxidized state of glutathione increased during drought in the WT and the aphenotypic line CIN1-8, but decreased in the other transgenic CIN1 lines (Fig. 6A). Apoplastic POX and SOD activities were strongly reduced by drought stress in the WT but were maintained or induced in CIN1 plants (Table 1). This was related to the performance of the different CIN1 lines and the electrolyte leakage (membrane stability) during the drought period. In this sense, cell membrane damage under drought stress was more severe in the WT and the aphenotypic CIN1-8 line than in the other CIN1 lines analysed (Fig. 6B). Proteomic analysis (Fig. 7; Table 2) confirmed a high expression for one POX isoform (spot 618) in CIN1 plants and other proteins related to plant stress defence responses: chitinase (spot 845) and proteases of the subtilisin-like clan (spots 393 and 414).


Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato.

Albacete A, Cantero-Navarro E, Großkinsky DK, Arias CL, Balibrea ME, Bru R, Fragner L, Ghanem ME, González Mde L, Hernández JA, Martínez-Andújar C, van der Graaff E, Weckwerth W, Zellnig G, Pérez-Alfocea F, Roitsch T - J. Exp. Bot. (2014)

Reference 2D electrophoretic pattern of mature leaf apoplast from WT plants and the CIN1-91 line growing under normal watering regimes and after 9 d of drought stress. Proteins were resolved using a linear pH gradient of 4–7 in the first dimension and 12% SDS–PAGE in the second dimension. Selected spots are indicated with arrows.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321548&req=5

Figure 7: Reference 2D electrophoretic pattern of mature leaf apoplast from WT plants and the CIN1-91 line growing under normal watering regimes and after 9 d of drought stress. Proteins were resolved using a linear pH gradient of 4–7 in the first dimension and 12% SDS–PAGE in the second dimension. Selected spots are indicated with arrows.
Mentions: During stress, disruption of cellular homeostasis is accompanied by the generation of reactive oxygen species (ROS), and the extent of stress-induced damage can be attenuated by the action of the cell’s antioxidant systems, including glutathione and enzymes capable of scavenging ROS. The oxidized state of glutathione increased during drought in the WT and the aphenotypic line CIN1-8, but decreased in the other transgenic CIN1 lines (Fig. 6A). Apoplastic POX and SOD activities were strongly reduced by drought stress in the WT but were maintained or induced in CIN1 plants (Table 1). This was related to the performance of the different CIN1 lines and the electrolyte leakage (membrane stability) during the drought period. In this sense, cell membrane damage under drought stress was more severe in the WT and the aphenotypic CIN1-8 line than in the other CIN1 lines analysed (Fig. 6B). Proteomic analysis (Fig. 7; Table 2) confirmed a high expression for one POX isoform (spot 618) in CIN1 plants and other proteins related to plant stress defence responses: chitinase (spot 845) and proteases of the subtilisin-like clan (spots 393 and 414).

Bottom Line: Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants.Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation.Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the senescence-delaying hormone trans-zeatin and decreases in the senescence-inducing ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in the leaves.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, CEBAS-CSIC, Campus de Espinardo, 30100 Murcia, Spain Institute of Plant Sciences, Department of Plant Physiology, University of Graz, 8010 Graz, Austria.

No MeSH data available.


Related in: MedlinePlus