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Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato.

Albacete A, Cantero-Navarro E, Großkinsky DK, Arias CL, Balibrea ME, Bru R, Fragner L, Ghanem ME, González Mde L, Hernández JA, Martínez-Andújar C, van der Graaff E, Weckwerth W, Zellnig G, Pérez-Alfocea F, Roitsch T - J. Exp. Bot. (2014)

Bottom Line: Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants.Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation.Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the senescence-delaying hormone trans-zeatin and decreases in the senescence-inducing ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in the leaves.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, CEBAS-CSIC, Campus de Espinardo, 30100 Murcia, Spain Institute of Plant Sciences, Department of Plant Physiology, University of Graz, 8010 Graz, Austria.

No MeSH data available.


Related in: MedlinePlus

Chlorophyll fluorescence imaging indicating the maximum quantum yield (Fv/Fm) of photosystem II in leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress (A), and evolution of leaf Fv/Fm during the drought period (B). Photosynthetic rate in leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress (C). Transmission electron microscopy images of tomato leaves subjected to 9 d of drought stress showing starch formation (D). Data are presented as means ±SE, *P<0.05, one-way ANOVA, n=3.
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Figure 3: Chlorophyll fluorescence imaging indicating the maximum quantum yield (Fv/Fm) of photosystem II in leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress (A), and evolution of leaf Fv/Fm during the drought period (B). Photosynthetic rate in leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress (C). Transmission electron microscopy images of tomato leaves subjected to 9 d of drought stress showing starch formation (D). Data are presented as means ±SE, *P<0.05, one-way ANOVA, n=3.

Mentions: Furthermore, regarding photosynthetic parameters, analyses of the spatio-temporal changes in (Fig. 3A) and absolute values of (Fig. 3B) Fv/Fm revealed that chlorophyll fluorescence was higher in the CIN1 plants than in the WT throughout the drought period. In agreement with this, the photosynthetic rate (A) was less reduced in CIN1 plants (30%) than in the WT plants (75%) and the aphenotypic line CIN1-8 (60%) (Fig. 3C). Interestingly, ultrastructural analysis by transmission electron microscopy revealed an increase in sugar storage since CIN1-91 leaves presented distinct and larger starch grains than the WT under drought stress (Fig. 3D).


Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato.

Albacete A, Cantero-Navarro E, Großkinsky DK, Arias CL, Balibrea ME, Bru R, Fragner L, Ghanem ME, González Mde L, Hernández JA, Martínez-Andújar C, van der Graaff E, Weckwerth W, Zellnig G, Pérez-Alfocea F, Roitsch T - J. Exp. Bot. (2014)

Chlorophyll fluorescence imaging indicating the maximum quantum yield (Fv/Fm) of photosystem II in leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress (A), and evolution of leaf Fv/Fm during the drought period (B). Photosynthetic rate in leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress (C). Transmission electron microscopy images of tomato leaves subjected to 9 d of drought stress showing starch formation (D). Data are presented as means ±SE, *P<0.05, one-way ANOVA, n=3.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321548&req=5

Figure 3: Chlorophyll fluorescence imaging indicating the maximum quantum yield (Fv/Fm) of photosystem II in leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress (A), and evolution of leaf Fv/Fm during the drought period (B). Photosynthetic rate in leaves of WT and CIN1 plants under normal watering regimes and after 9 d of drought stress (C). Transmission electron microscopy images of tomato leaves subjected to 9 d of drought stress showing starch formation (D). Data are presented as means ±SE, *P<0.05, one-way ANOVA, n=3.
Mentions: Furthermore, regarding photosynthetic parameters, analyses of the spatio-temporal changes in (Fig. 3A) and absolute values of (Fig. 3B) Fv/Fm revealed that chlorophyll fluorescence was higher in the CIN1 plants than in the WT throughout the drought period. In agreement with this, the photosynthetic rate (A) was less reduced in CIN1 plants (30%) than in the WT plants (75%) and the aphenotypic line CIN1-8 (60%) (Fig. 3C). Interestingly, ultrastructural analysis by transmission electron microscopy revealed an increase in sugar storage since CIN1-91 leaves presented distinct and larger starch grains than the WT under drought stress (Fig. 3D).

Bottom Line: Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants.Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation.Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the senescence-delaying hormone trans-zeatin and decreases in the senescence-inducing ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in the leaves.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Nutrition, CEBAS-CSIC, Campus de Espinardo, 30100 Murcia, Spain Institute of Plant Sciences, Department of Plant Physiology, University of Graz, 8010 Graz, Austria.

No MeSH data available.


Related in: MedlinePlus