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Natural variation in the expression of ORGANIC CATION TRANSPORTER 1 affects root length responses to cadaverine in Arabidopsis.

Strohm AK, Vaughn LM, Masson PH - J. Exp. Bot. (2014)

Bottom Line: Recombinant inbred lines, near isogenic lines, and a microarray were used to show that variation in ORGANIC CATION TRANSPORTER 1 (OCT1) is at least partially responsible for this difference.OCT1 expression was higher in Cvi than in Ler, and oct1 mutants were more sensitive to cadaverine than wild-type plants.These results suggest that decreased OCT1 expression confers cadaverine sensitivity in some accessions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Genetics, University of Wisconsin Madison, 425G Henry Mall, Madison, WI 53706, USA.

No MeSH data available.


Related in: MedlinePlus

OCT1 expression relative to the reference gene At1G58050 is higher in Cvi roots than in Ler roots. Seedlings were grown on 0 μM or 50 μM cadaverine. Error bars indicate ±SE among biological replicates, and OCT1 expression for Ler on control medium was set to 1. The asterisk indicates a significant difference in expression compared with Ler for the given condition (P<0.05, Student’s t-test). Differences within each accession between cadaverine treatments are not significant.
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Figure 4: OCT1 expression relative to the reference gene At1G58050 is higher in Cvi roots than in Ler roots. Seedlings were grown on 0 μM or 50 μM cadaverine. Error bars indicate ±SE among biological replicates, and OCT1 expression for Ler on control medium was set to 1. The asterisk indicates a significant difference in expression compared with Ler for the given condition (P<0.05, Student’s t-test). Differences within each accession between cadaverine treatments are not significant.

Mentions: OCT1 from Cvi and Ler genomic DNA was sequenced. No large insertions or deletions were found, and only one amino acid change was found: residue 431 in the second exon is serine in Cvi and alanine in Ler (Supplementary Table S2 at JXB online). Analysing the 2kb of sequence upstream of the start codon revealed numerous single nucleotide polymorphisms and small insertions but no large insertions or deletions (Supplementary Table S2). Based on these data, it was hypothesized that OCT1 expression differences contribute to root length responses to exogenous cadaverine. qRT-PCR data confirmed the microarray result and showed that OCT1 expression was ~14 times as high in Cvi roots as in Ler roots (Fig. 4). Cadaverine treatment did not significantly change OCT1 expression in either accession (Fig. 4).


Natural variation in the expression of ORGANIC CATION TRANSPORTER 1 affects root length responses to cadaverine in Arabidopsis.

Strohm AK, Vaughn LM, Masson PH - J. Exp. Bot. (2014)

OCT1 expression relative to the reference gene At1G58050 is higher in Cvi roots than in Ler roots. Seedlings were grown on 0 μM or 50 μM cadaverine. Error bars indicate ±SE among biological replicates, and OCT1 expression for Ler on control medium was set to 1. The asterisk indicates a significant difference in expression compared with Ler for the given condition (P<0.05, Student’s t-test). Differences within each accession between cadaverine treatments are not significant.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321547&req=5

Figure 4: OCT1 expression relative to the reference gene At1G58050 is higher in Cvi roots than in Ler roots. Seedlings were grown on 0 μM or 50 μM cadaverine. Error bars indicate ±SE among biological replicates, and OCT1 expression for Ler on control medium was set to 1. The asterisk indicates a significant difference in expression compared with Ler for the given condition (P<0.05, Student’s t-test). Differences within each accession between cadaverine treatments are not significant.
Mentions: OCT1 from Cvi and Ler genomic DNA was sequenced. No large insertions or deletions were found, and only one amino acid change was found: residue 431 in the second exon is serine in Cvi and alanine in Ler (Supplementary Table S2 at JXB online). Analysing the 2kb of sequence upstream of the start codon revealed numerous single nucleotide polymorphisms and small insertions but no large insertions or deletions (Supplementary Table S2). Based on these data, it was hypothesized that OCT1 expression differences contribute to root length responses to exogenous cadaverine. qRT-PCR data confirmed the microarray result and showed that OCT1 expression was ~14 times as high in Cvi roots as in Ler roots (Fig. 4). Cadaverine treatment did not significantly change OCT1 expression in either accession (Fig. 4).

Bottom Line: Recombinant inbred lines, near isogenic lines, and a microarray were used to show that variation in ORGANIC CATION TRANSPORTER 1 (OCT1) is at least partially responsible for this difference.OCT1 expression was higher in Cvi than in Ler, and oct1 mutants were more sensitive to cadaverine than wild-type plants.These results suggest that decreased OCT1 expression confers cadaverine sensitivity in some accessions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Genetics, University of Wisconsin Madison, 425G Henry Mall, Madison, WI 53706, USA.

No MeSH data available.


Related in: MedlinePlus