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Distinct patterns of the histone marks associated with recruitment of the methionine chain-elongation pathway from leucine biosynthesis.

Xue M, Long J, Jiang Q, Wang M, Chen S, Pang Q, He Y - J. Exp. Bot. (2014)

Bottom Line: In general, genes involved in leucine biosynthesis were robustly associated with H3k4me2 and H3K4me3.This H3K4m3-depleted pattern had no effect on gene transcription, whereas it seemingly co-evolved with the entire pathway of aliphatic GLS biosynthesis.The results reveal a novel association of the epigenetic marks with plant secondary metabolism, and may help to understand the recruitment of the methionine chain-elongation pathway from leucine biosynthesis.

View Article: PubMed Central - PubMed

Affiliation: National Maize Improvement Center of China, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China.

No MeSH data available.


Levels of H3K4m2 and H3K4me3 for genes in the aliphatic (A) and indolic (B) GLS pathway were determined by ChIP-qPCR analysis. The y-axis shows the fold ChIP enrichment over input. The data were from three biological replicates and are shown as mean values ±SD. (This figure is available in colour at JXB online.)
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Figure 6: Levels of H3K4m2 and H3K4me3 for genes in the aliphatic (A) and indolic (B) GLS pathway were determined by ChIP-qPCR analysis. The y-axis shows the fold ChIP enrichment over input. The data were from three biological replicates and are shown as mean values ±SD. (This figure is available in colour at JXB online.)

Mentions: The end-products of the methionine chain-elongation pathway serve as the precursors entering aliphatic GLS biosynthesis. To test the likelihood that the absence of H3K4me3 represents a common character co-ordinated in the aliphatic GLS biosynthetic pathway, the levels of H3K4me2 and H3K4me3 were assayed for all the genes involved in the formation of the aliphatic GLS core structure. As shown in Fig. 6A, no detectable H3K4me3 could be identified in most of these genes except for SUR1 and UGT74B1, two genes playing roles in both aliphatic and indolic GLS pathways. In contrast, a moderate level of occurrence of H3K4me2 was found in most of the genes (Fig. 6A). Three transcription factors (TFs), MYB28, MYB29, and MYB76, have been reported as master regulators modulating aliphatic GLS biosynthesis (Gigolashvili et al., 20072008; Hirai et al., 2007; Sonderby et al., 2007 2010b; Li et al., 2013). Previous studies have shown that these TFs were synergistically co-regulated with other biosynthetic genes at the transcriptional level (Hirai et al., 2007). Here it was found that the H3K4me3-depleted pattern was also conserved in these TFs (Fig. 6A), indicating that the genes involved in aliphatic GLS biosynthesis and the corresponding transcriptional regulation were co-ordinated at both the genomic and transcriptional level.


Distinct patterns of the histone marks associated with recruitment of the methionine chain-elongation pathway from leucine biosynthesis.

Xue M, Long J, Jiang Q, Wang M, Chen S, Pang Q, He Y - J. Exp. Bot. (2014)

Levels of H3K4m2 and H3K4me3 for genes in the aliphatic (A) and indolic (B) GLS pathway were determined by ChIP-qPCR analysis. The y-axis shows the fold ChIP enrichment over input. The data were from three biological replicates and are shown as mean values ±SD. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321544&req=5

Figure 6: Levels of H3K4m2 and H3K4me3 for genes in the aliphatic (A) and indolic (B) GLS pathway were determined by ChIP-qPCR analysis. The y-axis shows the fold ChIP enrichment over input. The data were from three biological replicates and are shown as mean values ±SD. (This figure is available in colour at JXB online.)
Mentions: The end-products of the methionine chain-elongation pathway serve as the precursors entering aliphatic GLS biosynthesis. To test the likelihood that the absence of H3K4me3 represents a common character co-ordinated in the aliphatic GLS biosynthetic pathway, the levels of H3K4me2 and H3K4me3 were assayed for all the genes involved in the formation of the aliphatic GLS core structure. As shown in Fig. 6A, no detectable H3K4me3 could be identified in most of these genes except for SUR1 and UGT74B1, two genes playing roles in both aliphatic and indolic GLS pathways. In contrast, a moderate level of occurrence of H3K4me2 was found in most of the genes (Fig. 6A). Three transcription factors (TFs), MYB28, MYB29, and MYB76, have been reported as master regulators modulating aliphatic GLS biosynthesis (Gigolashvili et al., 20072008; Hirai et al., 2007; Sonderby et al., 2007 2010b; Li et al., 2013). Previous studies have shown that these TFs were synergistically co-regulated with other biosynthetic genes at the transcriptional level (Hirai et al., 2007). Here it was found that the H3K4me3-depleted pattern was also conserved in these TFs (Fig. 6A), indicating that the genes involved in aliphatic GLS biosynthesis and the corresponding transcriptional regulation were co-ordinated at both the genomic and transcriptional level.

Bottom Line: In general, genes involved in leucine biosynthesis were robustly associated with H3k4me2 and H3K4me3.This H3K4m3-depleted pattern had no effect on gene transcription, whereas it seemingly co-evolved with the entire pathway of aliphatic GLS biosynthesis.The results reveal a novel association of the epigenetic marks with plant secondary metabolism, and may help to understand the recruitment of the methionine chain-elongation pathway from leucine biosynthesis.

View Article: PubMed Central - PubMed

Affiliation: National Maize Improvement Center of China, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China.

No MeSH data available.