Figure 9: Melatonin enhances expressions of photosynthesis-related genes under normal and salt stress conditions. (A) Expression of genes related to photosynthesis. Red colour indicates up-regulation and green colour indicates down-regulation. Quadrangle represents a comparison of melatonin treated versus water control; circle represents a comparison of salt versus water control: triangle represents a comparison of salt plus melatonin versus salt. (B) Relative gene expression level analysed by q-PCR. A GmTubulin fragment was amplified as an internal control. Bars indicate standard deviation (n=3).

Mentions: To confirm the results of transcriptome analysis, we extracted RNA from independently grown and treated plants and performed quantitative RT-PCR. The important genes that enriched in pathway analysis were tested and the real-time PCR results were consistent with transcriptome analysis (Figs 9B, 10B, 11B, and Supplementary Fig. S4B).