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Melatonin enhances plant growth and abiotic stress tolerance in soybean plants.

Wei W, Li QT, Chu YN, Reiter RJ, Yu XM, Zhu DH, Zhang WK, Ma B, Lin Q, Zhang JS, Chen SY - J. Exp. Bot. (2014)

Bottom Line: In the present study, we tested the effect of melatonin (N-acetyl-5-methoxytryptamine) on soybean growth and development.Transcriptome analysis revealed that salt stress inhibited expressions of genes related to binding, oxidoreductase activity/process, and secondary metabolic processes.Melatonin up-regulated expressions of the genes inhibited by salt stress, and hence alleviated the inhibitory effects of salt stress on gene expressions.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beichen West Road, Campus #1, No.2, Beijing 100101, China.

No MeSH data available.


Analysis of RNA-sequencing data. (A) Differentially expressed gene number. Mt:H2O identifies 100 µM melatonin-treated samples versus water controls, NaCl:H2O identifies 1% salt-treated samples versus water controls, NaCl+Mt:NaCl identifies salt- and melatonin-treated samples versus 1% salt- treated samples, NaCl+Mt:Mt identifies salt- and melatonin-treated samples versus melatonin-treated samples. (B) Gene numbers affected by various treatments. Up- and down-regulated genes (fold change≥2) were examined for common genes using Venn diagram. Overlapping areas represent common genes. NaCl+Mt:H2O identifies salt- and melatonin-treated samples versus water controls. (C) Comparison of gene numbers affected by different treatments using Venn diagram.
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Figure 6: Analysis of RNA-sequencing data. (A) Differentially expressed gene number. Mt:H2O identifies 100 µM melatonin-treated samples versus water controls, NaCl:H2O identifies 1% salt-treated samples versus water controls, NaCl+Mt:NaCl identifies salt- and melatonin-treated samples versus 1% salt- treated samples, NaCl+Mt:Mt identifies salt- and melatonin-treated samples versus melatonin-treated samples. (B) Gene numbers affected by various treatments. Up- and down-regulated genes (fold change≥2) were examined for common genes using Venn diagram. Overlapping areas represent common genes. NaCl+Mt:H2O identifies salt- and melatonin-treated samples versus water controls. (C) Comparison of gene numbers affected by different treatments using Venn diagram.

Mentions: To investigate the possible mechanism of the promotional roles of melatonin on soybean plants, transcriptome analysis was performed. Two-week old soybean seedlings were treated with water, 100 µM melatonin, 1% NaCl or 1% NaCl plus 100 µM melatonin, and RNAs were isolated for RNA-seq analysis. Statistics of clean reads in RNA sequencing are shown in Table 1. Four comparisons were conducted, including treatments of melatonin (Mt) versus water (Mt:H2O), salt versus water (NaCl:H2O), salt plus melatonin versus salt (NaCl+Mt:NaCl), and salt plus melatonin versus melatonin (NaCl+Mt:Mt). Compared with the transcripts of non-treated samples (water), melatonin-treated samples had 5503 up-regulated genes and 2162 down-regulated genes, whereas salt-treated samples had 524 up-regulated genes and 1146 down-regulated genes. Compared with salt-treated samples, NaCl+Mt samples had 1231 up-regulated genes and 233 down-regulated genes. Compared with melatonin-treated samples, NaCl+Mt samples had 1825 up-regulated genes and 4465 down-regulated genes (Fig. 6A). The heatmap by cluster analysis also revealed that melatonin enhanced the expression level of a large number of genes compared with the other three samples (Supplementary Fig. S2). Venn diagrams were used to analyse the relationship between different treatments. Compared with water samples, there were 28 genes up-regulated by all three treatments (Fig. 6B and Supplementary Table S1), suggesting that they may respond to environmental changes. It was presumed from the experiments above that melatonin may mitigate the effects of salt (Fig. 4 and Fig. 6A), and thus the regulation of gene expressions by melatonin and salt were analysed. There were 303 (Fig 6C, left: up in Mt:H2O versus up in NaCl+Mt:NaCl) genes commonly up-regulated and 14 (Fig 6C, right: down in Mt:H2O versus down in NaCl+Mt:NaCl) genes commonly down-regulated by melatonin in the absence and presence of salt. There were 75 (Fig 6C, left: down in NaCl:H2O versus down in NaCl+Mt:Mt) genes commonly down-regulated and 46 (Fig 6C, right: up in NaCl:H2O versus NaCl+Mt:Mt) genes commonly up-regulated by salt in the absence and presence of melatonin. Four comparisons could be divided into two groups, and each group contained two contrasting comparisons (Group I: Mt:H2O and NaCl+Mt:Mt, Group II: NaCl:H2O and NaCl+Mt:NaCl) (Fig. 6C). A reciprocal analysis was also performed and much fewer common genes were found (Supplementary Fig. S3). Details of the genes in the Venn diagrams (Fig. 6C) can be found in Supplementary Table S2.


Melatonin enhances plant growth and abiotic stress tolerance in soybean plants.

Wei W, Li QT, Chu YN, Reiter RJ, Yu XM, Zhu DH, Zhang WK, Ma B, Lin Q, Zhang JS, Chen SY - J. Exp. Bot. (2014)

Analysis of RNA-sequencing data. (A) Differentially expressed gene number. Mt:H2O identifies 100 µM melatonin-treated samples versus water controls, NaCl:H2O identifies 1% salt-treated samples versus water controls, NaCl+Mt:NaCl identifies salt- and melatonin-treated samples versus 1% salt- treated samples, NaCl+Mt:Mt identifies salt- and melatonin-treated samples versus melatonin-treated samples. (B) Gene numbers affected by various treatments. Up- and down-regulated genes (fold change≥2) were examined for common genes using Venn diagram. Overlapping areas represent common genes. NaCl+Mt:H2O identifies salt- and melatonin-treated samples versus water controls. (C) Comparison of gene numbers affected by different treatments using Venn diagram.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4321538&req=5

Figure 6: Analysis of RNA-sequencing data. (A) Differentially expressed gene number. Mt:H2O identifies 100 µM melatonin-treated samples versus water controls, NaCl:H2O identifies 1% salt-treated samples versus water controls, NaCl+Mt:NaCl identifies salt- and melatonin-treated samples versus 1% salt- treated samples, NaCl+Mt:Mt identifies salt- and melatonin-treated samples versus melatonin-treated samples. (B) Gene numbers affected by various treatments. Up- and down-regulated genes (fold change≥2) were examined for common genes using Venn diagram. Overlapping areas represent common genes. NaCl+Mt:H2O identifies salt- and melatonin-treated samples versus water controls. (C) Comparison of gene numbers affected by different treatments using Venn diagram.
Mentions: To investigate the possible mechanism of the promotional roles of melatonin on soybean plants, transcriptome analysis was performed. Two-week old soybean seedlings were treated with water, 100 µM melatonin, 1% NaCl or 1% NaCl plus 100 µM melatonin, and RNAs were isolated for RNA-seq analysis. Statistics of clean reads in RNA sequencing are shown in Table 1. Four comparisons were conducted, including treatments of melatonin (Mt) versus water (Mt:H2O), salt versus water (NaCl:H2O), salt plus melatonin versus salt (NaCl+Mt:NaCl), and salt plus melatonin versus melatonin (NaCl+Mt:Mt). Compared with the transcripts of non-treated samples (water), melatonin-treated samples had 5503 up-regulated genes and 2162 down-regulated genes, whereas salt-treated samples had 524 up-regulated genes and 1146 down-regulated genes. Compared with salt-treated samples, NaCl+Mt samples had 1231 up-regulated genes and 233 down-regulated genes. Compared with melatonin-treated samples, NaCl+Mt samples had 1825 up-regulated genes and 4465 down-regulated genes (Fig. 6A). The heatmap by cluster analysis also revealed that melatonin enhanced the expression level of a large number of genes compared with the other three samples (Supplementary Fig. S2). Venn diagrams were used to analyse the relationship between different treatments. Compared with water samples, there were 28 genes up-regulated by all three treatments (Fig. 6B and Supplementary Table S1), suggesting that they may respond to environmental changes. It was presumed from the experiments above that melatonin may mitigate the effects of salt (Fig. 4 and Fig. 6A), and thus the regulation of gene expressions by melatonin and salt were analysed. There were 303 (Fig 6C, left: up in Mt:H2O versus up in NaCl+Mt:NaCl) genes commonly up-regulated and 14 (Fig 6C, right: down in Mt:H2O versus down in NaCl+Mt:NaCl) genes commonly down-regulated by melatonin in the absence and presence of salt. There were 75 (Fig 6C, left: down in NaCl:H2O versus down in NaCl+Mt:Mt) genes commonly down-regulated and 46 (Fig 6C, right: up in NaCl:H2O versus NaCl+Mt:Mt) genes commonly up-regulated by salt in the absence and presence of melatonin. Four comparisons could be divided into two groups, and each group contained two contrasting comparisons (Group I: Mt:H2O and NaCl+Mt:Mt, Group II: NaCl:H2O and NaCl+Mt:NaCl) (Fig. 6C). A reciprocal analysis was also performed and much fewer common genes were found (Supplementary Fig. S3). Details of the genes in the Venn diagrams (Fig. 6C) can be found in Supplementary Table S2.

Bottom Line: In the present study, we tested the effect of melatonin (N-acetyl-5-methoxytryptamine) on soybean growth and development.Transcriptome analysis revealed that salt stress inhibited expressions of genes related to binding, oxidoreductase activity/process, and secondary metabolic processes.Melatonin up-regulated expressions of the genes inhibited by salt stress, and hence alleviated the inhibitory effects of salt stress on gene expressions.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beichen West Road, Campus #1, No.2, Beijing 100101, China.

No MeSH data available.