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Chloroplast-encoded serotonin N-acetyltransferase in the red alga Pyropia yezoensis: gene transition to the nucleus from chloroplasts.

Byeon Y, Yool Lee H, Choi DW, Back K - J. Exp. Bot. (2014)

Bottom Line: Melatonin was present at 0.16ng g(-1) of fresh mass but increased during heat stress.Phylogenetic analysis of the sequence suggested that PySNAT has evolved from the cyanobacteria SNAT gene via endosymbiotic gene transfer.Additionally, the chloroplast transit peptides of plant SNATs were acquired 1500 million years ago, concurrent with the appearance of green algae.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Interdisciplinary Program of Bioenergy and Biomaterials, Bioenergy Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.

No MeSH data available.


Related in: MedlinePlus

Subcellular localization of PySNAT. (A) Red fluorescence of PySNAT-mCherry. (B) Green fluorescence of cytoplasmic GFP. (C) Cyan fluorescence of chlorophyll. (D) The fluorescence images were merged. Agrobacterium-infiltrated tobacco (Nicotiana benthamiana) leaves with XVE-inducible PySNAT-mCherry were grown for 2 d in a growth room before XVE-induction and visualization by confocal microscopy. Bars, 20 μm. The GenBank accession number of PySNAT is NC_007932.
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Figure 5: Subcellular localization of PySNAT. (A) Red fluorescence of PySNAT-mCherry. (B) Green fluorescence of cytoplasmic GFP. (C) Cyan fluorescence of chlorophyll. (D) The fluorescence images were merged. Agrobacterium-infiltrated tobacco (Nicotiana benthamiana) leaves with XVE-inducible PySNAT-mCherry were grown for 2 d in a growth room before XVE-induction and visualization by confocal microscopy. Bars, 20 μm. The GenBank accession number of PySNAT is NC_007932.

Mentions: Based on the potential origin of PySNAT from cyanobacteria, PySNAT may target to chloroplasts in higher plants because some proteins (even in the absence of chloroplast transit peptides) can localize to chloroplasts (Ha et al., 2003; Jung et al., 2004). To determine whether PySNAT can target to chloroplasts in tobacco, we constructed a binary vector, pER8-PySNAT-mCherry, under the control of the oestrogen-inducible XVE promoter. Agrobacterium cells harbouring the binary vector pER8-PySNAT-mCherry were infiltrated into tobacco leaves to examine subcellular localization using confocal microscopy. As shown in Fig. 5, PySANT showed a strong red fluorescence in tobacco cytoplasm (Fig. 5A) that co-localized with the green fluorescence of GFP, a cytoplasmic marker protein (Fig. 5B). The red fluorescence of PySNAT merged with the green fluorescence of cytoplasmic GFP (Fig. 5D), which indicated that the chloroplast-encoded PySNAT is devoid of chloroplast transit peptides and lacks an intrinsic ability to translocate into chloroplasts of higher plants. Although the primary structure of PySNAT (including pI) differed from that of cyanobacteria SNAT, PySNAT itself cannot translocate into chloroplasts in higher plants and requires the acquisition of an N-terminal chloroplast transit peptide during evolution.


Chloroplast-encoded serotonin N-acetyltransferase in the red alga Pyropia yezoensis: gene transition to the nucleus from chloroplasts.

Byeon Y, Yool Lee H, Choi DW, Back K - J. Exp. Bot. (2014)

Subcellular localization of PySNAT. (A) Red fluorescence of PySNAT-mCherry. (B) Green fluorescence of cytoplasmic GFP. (C) Cyan fluorescence of chlorophyll. (D) The fluorescence images were merged. Agrobacterium-infiltrated tobacco (Nicotiana benthamiana) leaves with XVE-inducible PySNAT-mCherry were grown for 2 d in a growth room before XVE-induction and visualization by confocal microscopy. Bars, 20 μm. The GenBank accession number of PySNAT is NC_007932.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4321536&req=5

Figure 5: Subcellular localization of PySNAT. (A) Red fluorescence of PySNAT-mCherry. (B) Green fluorescence of cytoplasmic GFP. (C) Cyan fluorescence of chlorophyll. (D) The fluorescence images were merged. Agrobacterium-infiltrated tobacco (Nicotiana benthamiana) leaves with XVE-inducible PySNAT-mCherry were grown for 2 d in a growth room before XVE-induction and visualization by confocal microscopy. Bars, 20 μm. The GenBank accession number of PySNAT is NC_007932.
Mentions: Based on the potential origin of PySNAT from cyanobacteria, PySNAT may target to chloroplasts in higher plants because some proteins (even in the absence of chloroplast transit peptides) can localize to chloroplasts (Ha et al., 2003; Jung et al., 2004). To determine whether PySNAT can target to chloroplasts in tobacco, we constructed a binary vector, pER8-PySNAT-mCherry, under the control of the oestrogen-inducible XVE promoter. Agrobacterium cells harbouring the binary vector pER8-PySNAT-mCherry were infiltrated into tobacco leaves to examine subcellular localization using confocal microscopy. As shown in Fig. 5, PySANT showed a strong red fluorescence in tobacco cytoplasm (Fig. 5A) that co-localized with the green fluorescence of GFP, a cytoplasmic marker protein (Fig. 5B). The red fluorescence of PySNAT merged with the green fluorescence of cytoplasmic GFP (Fig. 5D), which indicated that the chloroplast-encoded PySNAT is devoid of chloroplast transit peptides and lacks an intrinsic ability to translocate into chloroplasts of higher plants. Although the primary structure of PySNAT (including pI) differed from that of cyanobacteria SNAT, PySNAT itself cannot translocate into chloroplasts in higher plants and requires the acquisition of an N-terminal chloroplast transit peptide during evolution.

Bottom Line: Melatonin was present at 0.16ng g(-1) of fresh mass but increased during heat stress.Phylogenetic analysis of the sequence suggested that PySNAT has evolved from the cyanobacteria SNAT gene via endosymbiotic gene transfer.Additionally, the chloroplast transit peptides of plant SNATs were acquired 1500 million years ago, concurrent with the appearance of green algae.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Interdisciplinary Program of Bioenergy and Biomaterials, Bioenergy Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.

No MeSH data available.


Related in: MedlinePlus