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Degradation of toluene by ortho cleavage enzymes in Burkholderia fungorum FLU100.

Dobslaw D, Engesser KH - Microb Biotechnol (2014)

Bottom Line: This means that 2-ML is an intermediate of toluene degradation and converted by specific enzymes.The conversion of 4-methylcatechol as a very minor by-product of toluene degradation in strain FLU100 resulted in the accumulation of 4-carboxymethyl-4-methylbut-2-en-4-olide (4-methyl-2-enelactone, 4-ML) as a dead-end product, excluding its nature as a possible intermediate.Thus, 3-methylcyclohexa-3,5-diene-1,2-diol, 3-methylcatechol, 2-methyl muconate and 2-ML were identified as central intermediates of productive ortho cleavage pathways for toluene metabolism in B. fungorum FLU100.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management, University of Stuttgart, Bandtäle 2, Stuttgart, D-70569, Germany.

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Concentration of 3-methylcyclohexa-3,5-diene-1,2-diol as substrate and its intermediates during conversion by strain FLU100 pre-grown on toluene. Additionally, values of optical density measured at 546 nm wavelength are given.
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fig01: Concentration of 3-methylcyclohexa-3,5-diene-1,2-diol as substrate and its intermediates during conversion by strain FLU100 pre-grown on toluene. Additionally, values of optical density measured at 546 nm wavelength are given.

Mentions: The conversion rate of the diendiol compound in comparison with conversion rates of 3-methylcatechol, 2-methylmuconic acid and 2-ML was extremely high. In case of 3-methylcyclohexa-3,5-diene-1,2-diol, nearly 0.6 mmol l−1 of the substrate, equivalent to 50% of the concentration supplied, was biologically converted to the corresponding catechol within 5 min (Fig. 1). Because of time delays of HPLC analyses, the conversion rate of diendiol between the second and third measurements was transferred to Table 1, showing absolute conversion rates for all relevant substrates and intermediates for cells pre-grown on toluene.


Degradation of toluene by ortho cleavage enzymes in Burkholderia fungorum FLU100.

Dobslaw D, Engesser KH - Microb Biotechnol (2014)

Concentration of 3-methylcyclohexa-3,5-diene-1,2-diol as substrate and its intermediates during conversion by strain FLU100 pre-grown on toluene. Additionally, values of optical density measured at 546 nm wavelength are given.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321380&req=5

fig01: Concentration of 3-methylcyclohexa-3,5-diene-1,2-diol as substrate and its intermediates during conversion by strain FLU100 pre-grown on toluene. Additionally, values of optical density measured at 546 nm wavelength are given.
Mentions: The conversion rate of the diendiol compound in comparison with conversion rates of 3-methylcatechol, 2-methylmuconic acid and 2-ML was extremely high. In case of 3-methylcyclohexa-3,5-diene-1,2-diol, nearly 0.6 mmol l−1 of the substrate, equivalent to 50% of the concentration supplied, was biologically converted to the corresponding catechol within 5 min (Fig. 1). Because of time delays of HPLC analyses, the conversion rate of diendiol between the second and third measurements was transferred to Table 1, showing absolute conversion rates for all relevant substrates and intermediates for cells pre-grown on toluene.

Bottom Line: This means that 2-ML is an intermediate of toluene degradation and converted by specific enzymes.The conversion of 4-methylcatechol as a very minor by-product of toluene degradation in strain FLU100 resulted in the accumulation of 4-carboxymethyl-4-methylbut-2-en-4-olide (4-methyl-2-enelactone, 4-ML) as a dead-end product, excluding its nature as a possible intermediate.Thus, 3-methylcyclohexa-3,5-diene-1,2-diol, 3-methylcatechol, 2-methyl muconate and 2-ML were identified as central intermediates of productive ortho cleavage pathways for toluene metabolism in B. fungorum FLU100.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management, University of Stuttgart, Bandtäle 2, Stuttgart, D-70569, Germany.

Show MeSH
Related in: MedlinePlus