Degradation of toluene by ortho cleavage enzymes in Burkholderia fungorum FLU100.
Bottom Line: This means that 2-ML is an intermediate of toluene degradation and converted by specific enzymes.The conversion of 4-methylcatechol as a very minor by-product of toluene degradation in strain FLU100 resulted in the accumulation of 4-carboxymethyl-4-methylbut-2-en-4-olide (4-methyl-2-enelactone, 4-ML) as a dead-end product, excluding its nature as a possible intermediate.Thus, 3-methylcyclohexa-3,5-diene-1,2-diol, 3-methylcatechol, 2-methyl muconate and 2-ML were identified as central intermediates of productive ortho cleavage pathways for toluene metabolism in B. fungorum FLU100.
Affiliation: Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management, University of Stuttgart, Bandtäle 2, Stuttgart, D-70569, Germany.Show MeSH
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Mentions: The conversion rate of the diendiol compound in comparison with conversion rates of 3-methylcatechol, 2-methylmuconic acid and 2-ML was extremely high. In case of 3-methylcyclohexa-3,5-diene-1,2-diol, nearly 0.6 mmol l−1 of the substrate, equivalent to 50% of the concentration supplied, was biologically converted to the corresponding catechol within 5 min (Fig. 1). Because of time delays of HPLC analyses, the conversion rate of diendiol between the second and third measurements was transferred to Table 1, showing absolute conversion rates for all relevant substrates and intermediates for cells pre-grown on toluene.
Affiliation: Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management, University of Stuttgart, Bandtäle 2, Stuttgart, D-70569, Germany.